Cargando…

Counting on co-transcriptional splicing

Splicing is the removal of intron sequences from pre-mRNA by the spliceosome. Researchers working in multiple model organisms – notably yeast, insects and mammalian cells – have shown that pre-mRNA can be spliced during the process of transcription (i.e. co-transcriptionally), as well as after trans...

Descripción completa

Detalles Bibliográficos
Autores principales: Brugiolo, Mattia, Herzel, Lydia, Neugebauer, Karla M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Faculty of 1000 Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3619158/
https://www.ncbi.nlm.nih.gov/pubmed/23638305
http://dx.doi.org/10.12703/P5-9
_version_ 1782265476657709056
author Brugiolo, Mattia
Herzel, Lydia
Neugebauer, Karla M.
author_facet Brugiolo, Mattia
Herzel, Lydia
Neugebauer, Karla M.
author_sort Brugiolo, Mattia
collection PubMed
description Splicing is the removal of intron sequences from pre-mRNA by the spliceosome. Researchers working in multiple model organisms – notably yeast, insects and mammalian cells – have shown that pre-mRNA can be spliced during the process of transcription (i.e. co-transcriptionally), as well as after transcription termination (i.e. post-transcriptionally). Co-transcriptional splicing does not assume that transcription and splicing machineries are mechanistically coupled, yet it raises this possibility. Early studies were based on a limited number of genes, which were often chosen because of their experimental accessibility. Since 2010, eight studies have used global datasets as counting tools, in order to quantify co-transcriptional intron removal. The consensus view, based on four organisms, is that the majority of splicing events take place co-transcriptionally in most cells and tissues. Here, we discuss the nature of the various global datasets and how bioinformatic analyses were conducted. Considering the broad differences in experimental approach and analysis, the level of agreement on the prevalence of co-transcriptional splicing is remarkable.
format Online
Article
Text
id pubmed-3619158
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Faculty of 1000 Ltd
record_format MEDLINE/PubMed
spelling pubmed-36191582013-05-01 Counting on co-transcriptional splicing Brugiolo, Mattia Herzel, Lydia Neugebauer, Karla M. F1000Prime Rep Review Article Splicing is the removal of intron sequences from pre-mRNA by the spliceosome. Researchers working in multiple model organisms – notably yeast, insects and mammalian cells – have shown that pre-mRNA can be spliced during the process of transcription (i.e. co-transcriptionally), as well as after transcription termination (i.e. post-transcriptionally). Co-transcriptional splicing does not assume that transcription and splicing machineries are mechanistically coupled, yet it raises this possibility. Early studies were based on a limited number of genes, which were often chosen because of their experimental accessibility. Since 2010, eight studies have used global datasets as counting tools, in order to quantify co-transcriptional intron removal. The consensus view, based on four organisms, is that the majority of splicing events take place co-transcriptionally in most cells and tissues. Here, we discuss the nature of the various global datasets and how bioinformatic analyses were conducted. Considering the broad differences in experimental approach and analysis, the level of agreement on the prevalence of co-transcriptional splicing is remarkable. Faculty of 1000 Ltd 2013-04-02 /pmc/articles/PMC3619158/ /pubmed/23638305 http://dx.doi.org/10.12703/P5-9 Text en © 2013 Faculty of 1000 Ltd http://creativecommons.org/licenses/by-nc/3.0/legalcode This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. You may not use this work for commercial purposes
spellingShingle Review Article
Brugiolo, Mattia
Herzel, Lydia
Neugebauer, Karla M.
Counting on co-transcriptional splicing
title Counting on co-transcriptional splicing
title_full Counting on co-transcriptional splicing
title_fullStr Counting on co-transcriptional splicing
title_full_unstemmed Counting on co-transcriptional splicing
title_short Counting on co-transcriptional splicing
title_sort counting on co-transcriptional splicing
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3619158/
https://www.ncbi.nlm.nih.gov/pubmed/23638305
http://dx.doi.org/10.12703/P5-9
work_keys_str_mv AT brugiolomattia countingoncotranscriptionalsplicing
AT herzellydia countingoncotranscriptionalsplicing
AT neugebauerkarlam countingoncotranscriptionalsplicing