A fluorescent reporter for mapping cellular protein-protein interactions in time and space

We introduce a fluorescent reporter for monitoring protein–protein interactions in living cells. The method is based on the Split-Ubiquitin method and uses the ratio of two auto-fluorescent reporter proteins as signal for interaction (SPLIFF). The mating of two haploid yeast cells initiates the anal...

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Detalles Bibliográficos
Autores principales: Moreno, Daniel, Neller, Joachim, Kestler, Hans A, Kraus, Johann, Dünkler, Alexander, Johnsson, Nils
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Molecular Biology Organization 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3619943/
https://www.ncbi.nlm.nih.gov/pubmed/23511205
http://dx.doi.org/10.1038/msb.2013.3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3619943/
https://www.ncbi.nlm.nih.gov/pubmed/23511205
http://dx.doi.org/10.1038/msb.2013.3

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