Cargando…
A fluorescent reporter for mapping cellular protein-protein interactions in time and space
We introduce a fluorescent reporter for monitoring protein–protein interactions in living cells. The method is based on the Split-Ubiquitin method and uses the ratio of two auto-fluorescent reporter proteins as signal for interaction (SPLIFF). The mating of two haploid yeast cells initiates the anal...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
European Molecular Biology Organization
2013
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3619943/ https://www.ncbi.nlm.nih.gov/pubmed/23511205 http://dx.doi.org/10.1038/msb.2013.3 |