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Detection of Transgenic and Endogenous Plant DNA Fragments and Proteins in the Digesta, Blood, Tissues, and Eggs of Laying Hens Fed with Phytase Transgenic Corn
The trials were conducted to assess the effects of long-term feeding with phytase transgenic corn (PTC) to hens on laying performance and egg quality, and investigate the fate of transgenic DNA and protein in digesta, blood, tissues, and eggs. Fifty-week old laying hens (n = 144) were fed with a die...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3620109/ https://www.ncbi.nlm.nih.gov/pubmed/23577200 http://dx.doi.org/10.1371/journal.pone.0061138 |
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author | Ma, Qiugang Gao, Chunqi Zhang, Jianyun Zhao, Lihong Hao, Wenbo Ji, Cheng |
author_facet | Ma, Qiugang Gao, Chunqi Zhang, Jianyun Zhao, Lihong Hao, Wenbo Ji, Cheng |
author_sort | Ma, Qiugang |
collection | PubMed |
description | The trials were conducted to assess the effects of long-term feeding with phytase transgenic corn (PTC) to hens on laying performance and egg quality, and investigate the fate of transgenic DNA and protein in digesta, blood, tissues, and eggs. Fifty-week old laying hens (n = 144) were fed with a diet containing 62.4% PTC or non-transgenic isogenic control corn (CC) for 16 weeks. We observed that feeding PTC to laying hens had no adverse effect on laying performance or egg quality (P>0.05) except on yolk color (P<0.05). Transgenic phyA2 gene and protein were rapidly degraded in the digestive tract and were not detected in blood, heart, liver, spleen, kidney, breast muscle, and eggs of laying hens fed with diet containing PTC. It was concluded that performance of hens fed diets containing PTC, as measured by egg production and egg quality, was similar to that of hens fed diets formulated with CC. There was no evidence of phyA2 gene or protein translocation to the blood, tissues, and eggs of laying hens. |
format | Online Article Text |
id | pubmed-3620109 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36201092013-04-10 Detection of Transgenic and Endogenous Plant DNA Fragments and Proteins in the Digesta, Blood, Tissues, and Eggs of Laying Hens Fed with Phytase Transgenic Corn Ma, Qiugang Gao, Chunqi Zhang, Jianyun Zhao, Lihong Hao, Wenbo Ji, Cheng PLoS One Research Article The trials were conducted to assess the effects of long-term feeding with phytase transgenic corn (PTC) to hens on laying performance and egg quality, and investigate the fate of transgenic DNA and protein in digesta, blood, tissues, and eggs. Fifty-week old laying hens (n = 144) were fed with a diet containing 62.4% PTC or non-transgenic isogenic control corn (CC) for 16 weeks. We observed that feeding PTC to laying hens had no adverse effect on laying performance or egg quality (P>0.05) except on yolk color (P<0.05). Transgenic phyA2 gene and protein were rapidly degraded in the digestive tract and were not detected in blood, heart, liver, spleen, kidney, breast muscle, and eggs of laying hens fed with diet containing PTC. It was concluded that performance of hens fed diets containing PTC, as measured by egg production and egg quality, was similar to that of hens fed diets formulated with CC. There was no evidence of phyA2 gene or protein translocation to the blood, tissues, and eggs of laying hens. Public Library of Science 2013-04-08 /pmc/articles/PMC3620109/ /pubmed/23577200 http://dx.doi.org/10.1371/journal.pone.0061138 Text en © 2013 Ma et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Ma, Qiugang Gao, Chunqi Zhang, Jianyun Zhao, Lihong Hao, Wenbo Ji, Cheng Detection of Transgenic and Endogenous Plant DNA Fragments and Proteins in the Digesta, Blood, Tissues, and Eggs of Laying Hens Fed with Phytase Transgenic Corn |
title | Detection of Transgenic and Endogenous Plant DNA Fragments and Proteins in the Digesta, Blood, Tissues, and Eggs of Laying Hens Fed with Phytase Transgenic Corn |
title_full | Detection of Transgenic and Endogenous Plant DNA Fragments and Proteins in the Digesta, Blood, Tissues, and Eggs of Laying Hens Fed with Phytase Transgenic Corn |
title_fullStr | Detection of Transgenic and Endogenous Plant DNA Fragments and Proteins in the Digesta, Blood, Tissues, and Eggs of Laying Hens Fed with Phytase Transgenic Corn |
title_full_unstemmed | Detection of Transgenic and Endogenous Plant DNA Fragments and Proteins in the Digesta, Blood, Tissues, and Eggs of Laying Hens Fed with Phytase Transgenic Corn |
title_short | Detection of Transgenic and Endogenous Plant DNA Fragments and Proteins in the Digesta, Blood, Tissues, and Eggs of Laying Hens Fed with Phytase Transgenic Corn |
title_sort | detection of transgenic and endogenous plant dna fragments and proteins in the digesta, blood, tissues, and eggs of laying hens fed with phytase transgenic corn |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3620109/ https://www.ncbi.nlm.nih.gov/pubmed/23577200 http://dx.doi.org/10.1371/journal.pone.0061138 |
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