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Sidenafil Pre-Treatment Promotes Decompression Sickness in Rats

Vascular bubble formation after decompression contributes to endothelial injuries which form the basis for the development of decompression sickness (DCS). Nitric oxide (NO) is a powerful vasodilator that contributes to vessel homeostasis. It has been shown that NO-releasing agent may reduce bubble...

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Autores principales: Blatteau, Jean-Eric, Brubakk, Alf O., Gempp, Emmanuel, Castagna, Olivier, Risso, Jean-Jacques, Vallée, Nicolas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3620342/
https://www.ncbi.nlm.nih.gov/pubmed/23580342
http://dx.doi.org/10.1371/journal.pone.0060639
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author Blatteau, Jean-Eric
Brubakk, Alf O.
Gempp, Emmanuel
Castagna, Olivier
Risso, Jean-Jacques
Vallée, Nicolas
author_facet Blatteau, Jean-Eric
Brubakk, Alf O.
Gempp, Emmanuel
Castagna, Olivier
Risso, Jean-Jacques
Vallée, Nicolas
author_sort Blatteau, Jean-Eric
collection PubMed
description Vascular bubble formation after decompression contributes to endothelial injuries which form the basis for the development of decompression sickness (DCS). Nitric oxide (NO) is a powerful vasodilator that contributes to vessel homeostasis. It has been shown that NO-releasing agent may reduce bubble formation and prevent serious decompression sickness. The use of sildenafil, a well-known, phosphodiesterase-5 blocker, which act by potentiating the vasodilatory effect on smooth muscle relaxation, has never been studied in DCS. The purpose of the present study was to evaluate the clinical effects of sildenafil pre-treatment on DCS in a rat model. 67 rats were subjected to a simulated dive at 90 msw for 45 min before staged decompression. The experimental group received 10 mg/kg of sildenafil one hour before exposure (n = 35) while controls were not treated (n = 32). Clinical assessment took place over a period of 30 min after surfacing. At the end, blood samples were collected for blood cells counts and the level of circulating bubbles in the right cavities was quantified. There were significantly more manifestations of DCS in the sildenafil group than in the controls (34.3% vs 6.25%, respectively, p = 0.012). Platelet count was more reduced in treated rats than in controls (−21.7% vs −7%, respectively, p = 0.029), whereas bubble grades did not differ between groups. We concluded that pre-treatment with sildenafil promotes the onset and severity of neurological DCS. When considering the use of phosphodiesterase-5 blockers in the context of diving, careful discussion with physician should be recommended.
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spelling pubmed-36203422013-04-11 Sidenafil Pre-Treatment Promotes Decompression Sickness in Rats Blatteau, Jean-Eric Brubakk, Alf O. Gempp, Emmanuel Castagna, Olivier Risso, Jean-Jacques Vallée, Nicolas PLoS One Research Article Vascular bubble formation after decompression contributes to endothelial injuries which form the basis for the development of decompression sickness (DCS). Nitric oxide (NO) is a powerful vasodilator that contributes to vessel homeostasis. It has been shown that NO-releasing agent may reduce bubble formation and prevent serious decompression sickness. The use of sildenafil, a well-known, phosphodiesterase-5 blocker, which act by potentiating the vasodilatory effect on smooth muscle relaxation, has never been studied in DCS. The purpose of the present study was to evaluate the clinical effects of sildenafil pre-treatment on DCS in a rat model. 67 rats were subjected to a simulated dive at 90 msw for 45 min before staged decompression. The experimental group received 10 mg/kg of sildenafil one hour before exposure (n = 35) while controls were not treated (n = 32). Clinical assessment took place over a period of 30 min after surfacing. At the end, blood samples were collected for blood cells counts and the level of circulating bubbles in the right cavities was quantified. There were significantly more manifestations of DCS in the sildenafil group than in the controls (34.3% vs 6.25%, respectively, p = 0.012). Platelet count was more reduced in treated rats than in controls (−21.7% vs −7%, respectively, p = 0.029), whereas bubble grades did not differ between groups. We concluded that pre-treatment with sildenafil promotes the onset and severity of neurological DCS. When considering the use of phosphodiesterase-5 blockers in the context of diving, careful discussion with physician should be recommended. Public Library of Science 2013-04-08 /pmc/articles/PMC3620342/ /pubmed/23580342 http://dx.doi.org/10.1371/journal.pone.0060639 Text en © 2013 Blatteau et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Blatteau, Jean-Eric
Brubakk, Alf O.
Gempp, Emmanuel
Castagna, Olivier
Risso, Jean-Jacques
Vallée, Nicolas
Sidenafil Pre-Treatment Promotes Decompression Sickness in Rats
title Sidenafil Pre-Treatment Promotes Decompression Sickness in Rats
title_full Sidenafil Pre-Treatment Promotes Decompression Sickness in Rats
title_fullStr Sidenafil Pre-Treatment Promotes Decompression Sickness in Rats
title_full_unstemmed Sidenafil Pre-Treatment Promotes Decompression Sickness in Rats
title_short Sidenafil Pre-Treatment Promotes Decompression Sickness in Rats
title_sort sidenafil pre-treatment promotes decompression sickness in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3620342/
https://www.ncbi.nlm.nih.gov/pubmed/23580342
http://dx.doi.org/10.1371/journal.pone.0060639
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