Cargando…

A comparative analysis of the properties of regulated promoter systems commonly used for recombinant gene expression in Escherichia coli

BACKGROUND: Production of recombinant proteins in bacteria for academic and commercial purposes is a well established field; however the outcomes of process developments for specific proteins are still often unpredictable. One reason is the limited understanding of the performance of expression cass...

Descripción completa

Detalles Bibliográficos
Autores principales:	Balzer, Simone, Kucharova, Veronika, Megerle, Judith, Lale, Rahmi, Brautaset, Trygve, Valla, Svein
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2013
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3621392/ https://www.ncbi.nlm.nih.gov/pubmed/23506076 http://dx.doi.org/10.1186/1475-2859-12-26

Ejemplares similares

Non-Invasive Analysis of Recombinant mRNA Stability in Escherichia coli by a Combination of Transcriptional Inducer Wash-Out and qRT-PCR
por: Kucharova, Veronika, et al.
Publicado: (2013)

Positively regulated bacterial expression systems
por: Brautaset, Trygve, et al.
Publicado: (2009)

Strong stimulation of recombinant protein production in Escherichia coli by combining stimulatory control elements in an expression cassette
por: Zwick, Friederike, et al.
Publicado: (2012)

Special Issue: Recombinant Protein Expression in Microorganisms
por: Brautaset, Trygve, et al.
Publicado: (2019)

The expression of recombinant genes in Escherichia coli can be strongly stimulated at the transcript production level by mutating the DNA‐region corresponding to the 5′‐untranslated part of mRNA
por: Berg, Laila, et al.
Publicado: (2009)

The XylS/Pm regulator/promoter system and its use in fundamental studies of bacterial gene expression, recombinant protein production and metabolic engineering
por: Gawin, Agnieszka, et al.
Publicado: (2017)

Directed evolution of the transcription factor XylS for development of improved expression systems
por: Vee Aune, Trond Erik, et al.
Publicado: (2010)

Regulation of the expression level of transcription factor XylS reveals new functional insight into its induction mechanism at the Pm promoter
por: Zwick, Friederike, et al.
Publicado: (2013)

Combinatorial Mutagenesis and Selection to Understand and Improve Yeast Promoters
por: Berg, Laila, et al.
Publicado: (2013)

Translational regulation of periplasmic folding assistants and proteases as a valuable strategy to improve production of translocated recombinant proteins in Escherichia coli
por: Gawin, Agnieszka, et al.
Publicado: (2020)

A New and Improved Host-Independent Plasmid System for RK2-Based Conjugal Transfer
por: Strand, Trine Aakvik, et al.
Publicado: (2014)

Coordination of Metabolism and Virulence Factors Expression of Extraintestinal Pathogenic Escherichia coli Purified from Blood Cultures of Patients with Sepsis
por: Pettersen, Veronika Kuchařová, et al.
Publicado: (2016)

Dual UTR-A novel 5′ untranslated region design for synthetic biology applications
por: Balzer Le, Simone, et al.
Publicado: (2020)

Genetic Tools and Techniques for Recombinant Expression in Thermophilic Bacillaceae
por: Drejer, Eivind B., et al.
Publicado: (2018)

Evaluation of Heterologous Biosynthetic Pathways for Methanol-Based 5-Aminovalerate Production by Thermophilic Bacillus methanolicus
por: Brito, Luciana Fernandes, et al.
Publicado: (2021)

Establishment of a functional system for recombinant production of secreted proteins at 50 °C in the thermophilic Bacillus methanolicus
por: Irla, Marta, et al.
Publicado: (2020)

Expression and Purification of Recombinant Hemoglobin in Escherichia coli
por: Natarajan, Chandrasekhar, et al.
Publicado: (2011)

Hydrogen production by recombinant Escherichia coli strains
por: Maeda, Toshinari, et al.
Publicado: (2012)

A Recombinant Snake Cathelicidin Derivative Peptide: Antibiofilm Properties and Expression in Escherichia coli
por: Tajbakhsh, Mercedeh, et al.
Publicado: (2018)

A comparative approach to recombinantly produce the plant enzyme horseradish peroxidase in Escherichia coli
por: Gundinger, Thomas, et al.
Publicado: (2017)

Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli
por: Sørensen, Hans Peter, et al.
Publicado: (2005)

Obscured phylogeny and possible recombinational dormancy in Escherichia coli
por: Leopold, Shana R, et al.
Publicado: (2011)

Recombination Phenotypes of Escherichia coli greA Mutants
por: Poteete, Anthony R
Publicado: (2011)

High level production of tyrosinase in recombinant Escherichia coli
por: Ren, Qun, et al.
Publicado: (2013)

Recombinant Production of Human Interleukin 6 in Escherichia coli
por: Nausch, Henrik, et al.
Publicado: (2013)

Recombinant protein expression in Escherichia coli: advances and challenges
por: Rosano, Germán L., et al.
Publicado: (2014)

Isolating Escherichia coli strains for recombinant protein production
por: Schlegel, Susan, et al.
Publicado: (2016)

Cloning, expression and purification of recombinant dermatopontin in Escherichia coli
por: Seetaraman Amritha, Trikkur Madom, et al.
Publicado: (2020)

Immunogenic Domains and Secondary Structure of Escherichia coli Recombinant Secreted Protein Escherichia coli-Secreted Protein B
por: Caetano, Bruna Alves, et al.
Publicado: (2017)

Streamlining recombination-mediated genetic engineering by validating three neutral integration sites in Synechococcus sp. PCC 7002
por: Vogel, Anne Ilse Maria, et al.
Publicado: (2017)

Homologous recombination prevents methylation-induced toxicity in Escherichia coli
por: Nowosielska, Anetta, et al.
Publicado: (2006)

Response surface methodology of nitrilase production by recombinant Escherichia coli
por: Dubey, Sachin, et al.
Publicado: (2011)

Optimization of production of recombinant human growth hormone in Escherichia coli
por: Rezaei, Marzieh, et al.
Publicado: (2012)

Detoxifying Escherichia coli for endotoxin-free production of recombinant proteins
por: Mamat, Uwe, et al.
Publicado: (2015)

Comparison of Three Escherichia coli Strains in Recombinant Production of Reteplase
por: Fathi-Roudsari, Mehrnoosh, et al.
Publicado: (2016)

Genome engineering for improved recombinant protein expression in Escherichia coli
por: Mahalik, Shubhashree, et al.
Publicado: (2014)

Recombinant expression of Streptococcus pneumoniae capsular polysaccharides in Escherichia coli
por: Kay, Emily J., et al.
Publicado: (2016)

Optimal Cloning of PCR Fragments by Homologous Recombination in Escherichia coli
por: Jacobus, Ana Paula, et al.
Publicado: (2015)

Preparation of umami octopeptide with recombined Escherichia coli: Feasibility and challenges
por: Zhao, Liming, et al.
Publicado: (2017)

Recombinant biosynthesis of bacterial cellulose in genetically modified Escherichia coli
por: Buldum, Gizem, et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_4ao7845jmmicdsu1mle2h7d7u7