Bone marrow non-mesenchymal mononuclear cells induce functional differentiation of neuroblastoma cells

Less is known about the non-mesenchymal mononuclear cell fraction of human bone marrow on functional adaptation of neuroblastoma cells. Using immunocytochemistry, we showed that bone-marrow mononuclear cell (BMMC)-conditioned medium can induce tyrosine hydroxylase expression in neuroblastoma cells,...

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Autores principales: Phruksaniyom, Chareerut, Dharmasaroja, Permphan, Issaragrisil, Surapol
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Letter to the Editor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3621518/
https://www.ncbi.nlm.nih.gov/pubmed/23551916
http://dx.doi.org/10.1186/2162-3619-2-9
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author Phruksaniyom, Chareerut
Dharmasaroja, Permphan
Issaragrisil, Surapol
author_facet Phruksaniyom, Chareerut
Dharmasaroja, Permphan
Issaragrisil, Surapol
author_sort Phruksaniyom, Chareerut
collection PubMed
description Less is known about the non-mesenchymal mononuclear cell fraction of human bone marrow on functional adaptation of neuroblastoma cells. Using immunocytochemistry, we showed that bone-marrow mononuclear cell (BMMC)-conditioned medium can induce tyrosine hydroxylase expression in neuroblastoma cells, which is similar to the effect of retinoic acid. Using quantitative RT-PCR, we showed that NGF, CNTF, and BDNF mRNAs were detected in unfractionated BMMC populations from all human donors at different expression levels. Our results suggest that cells of the non-mesenchymal mononuclear cell fraction can induce functional adaptation of neuroblastoma cells, probably via their secreted trophic factors.
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spelling pubmed-36215182013-04-10 Bone marrow non-mesenchymal mononuclear cells induce functional differentiation of neuroblastoma cells Phruksaniyom, Chareerut Dharmasaroja, Permphan Issaragrisil, Surapol Exp Hematol Oncol Letter to the Editor Less is known about the non-mesenchymal mononuclear cell fraction of human bone marrow on functional adaptation of neuroblastoma cells. Using immunocytochemistry, we showed that bone-marrow mononuclear cell (BMMC)-conditioned medium can induce tyrosine hydroxylase expression in neuroblastoma cells, which is similar to the effect of retinoic acid. Using quantitative RT-PCR, we showed that NGF, CNTF, and BDNF mRNAs were detected in unfractionated BMMC populations from all human donors at different expression levels. Our results suggest that cells of the non-mesenchymal mononuclear cell fraction can induce functional adaptation of neuroblastoma cells, probably via their secreted trophic factors. BioMed Central 2013-04-03 /pmc/articles/PMC3621518/ /pubmed/23551916 http://dx.doi.org/10.1186/2162-3619-2-9 Text en Copyright © 2013 Phruksaniyom et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Letter to the Editor
Phruksaniyom, Chareerut
Dharmasaroja, Permphan
Issaragrisil, Surapol
Bone marrow non-mesenchymal mononuclear cells induce functional differentiation of neuroblastoma cells
title Bone marrow non-mesenchymal mononuclear cells induce functional differentiation of neuroblastoma cells
title_full Bone marrow non-mesenchymal mononuclear cells induce functional differentiation of neuroblastoma cells
title_fullStr Bone marrow non-mesenchymal mononuclear cells induce functional differentiation of neuroblastoma cells
title_full_unstemmed Bone marrow non-mesenchymal mononuclear cells induce functional differentiation of neuroblastoma cells
title_short Bone marrow non-mesenchymal mononuclear cells induce functional differentiation of neuroblastoma cells
title_sort bone marrow non-mesenchymal mononuclear cells induce functional differentiation of neuroblastoma cells
topic Letter to the Editor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3621518/
https://www.ncbi.nlm.nih.gov/pubmed/23551916
http://dx.doi.org/10.1186/2162-3619-2-9
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