Cargando…

The impact of diurnal variation on induced sputum cell counts in healthy adults

BACKGROUND: Induced sputum cell counts are a non-invasive, reliable method for evaluating the presence, type, and degree of inflammation in the airways of the lungs. Current reference values for induced sputum cell counts in healthy adults do not account for the effects of circadian rhythm, includin...

Descripción completa

Detalles Bibliográficos
Autores principales: Davidson, Warren J, Wong, Lisa E, The, Stephanie, Leigh, Richard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3621557/
https://www.ncbi.nlm.nih.gov/pubmed/23537329
http://dx.doi.org/10.1186/2045-7022-3-8
_version_ 1782265721418416128
author Davidson, Warren J
Wong, Lisa E
The, Stephanie
Leigh, Richard
author_facet Davidson, Warren J
Wong, Lisa E
The, Stephanie
Leigh, Richard
author_sort Davidson, Warren J
collection PubMed
description BACKGROUND: Induced sputum cell counts are a non-invasive, reliable method for evaluating the presence, type, and degree of inflammation in the airways of the lungs. Current reference values for induced sputum cell counts in healthy adults do not account for the effects of circadian rhythm, including diurnal variation. The objective of this study was to describe the diurnal variation in induced sputum cell counts, compared between early morning and late afternoon, in healthy adult individuals. METHODS: 100 healthy adult subjects with no history of lung disease and normal bronchial reactivity proceeded with induced sputum testing at 7 am and 4 pm on different days. The order of testing was randomized. The cytotechnologist preparing and performing the cell counts was blinded to the sample collection time and subject characteristics. RESULTS: 65 subjects were included in the final analyses. There was no significant change in the total and differential sputum cell counts between the 7 am and 4 pm collections. There was good inter-observer agreement with respect to differential sputum cell count interpretation. CONCLUSIONS: This is the largest study to assess the variation in induced sputum cell counts in healthy adult subjects at different times of the day. We found no significant change in total and differential sputum cell counts between the 7 am and 4 pm collection time points. This is in contrast to studies in asthmatics that demonstrated a circadian variation in sputum cell counts and other markers of inflammation, suggesting that fluctuations in airway inflammatory cells during the day are a disease-specific effect.
format Online
Article
Text
id pubmed-3621557
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-36215572013-04-10 The impact of diurnal variation on induced sputum cell counts in healthy adults Davidson, Warren J Wong, Lisa E The, Stephanie Leigh, Richard Clin Transl Allergy Research BACKGROUND: Induced sputum cell counts are a non-invasive, reliable method for evaluating the presence, type, and degree of inflammation in the airways of the lungs. Current reference values for induced sputum cell counts in healthy adults do not account for the effects of circadian rhythm, including diurnal variation. The objective of this study was to describe the diurnal variation in induced sputum cell counts, compared between early morning and late afternoon, in healthy adult individuals. METHODS: 100 healthy adult subjects with no history of lung disease and normal bronchial reactivity proceeded with induced sputum testing at 7 am and 4 pm on different days. The order of testing was randomized. The cytotechnologist preparing and performing the cell counts was blinded to the sample collection time and subject characteristics. RESULTS: 65 subjects were included in the final analyses. There was no significant change in the total and differential sputum cell counts between the 7 am and 4 pm collections. There was good inter-observer agreement with respect to differential sputum cell count interpretation. CONCLUSIONS: This is the largest study to assess the variation in induced sputum cell counts in healthy adult subjects at different times of the day. We found no significant change in total and differential sputum cell counts between the 7 am and 4 pm collection time points. This is in contrast to studies in asthmatics that demonstrated a circadian variation in sputum cell counts and other markers of inflammation, suggesting that fluctuations in airway inflammatory cells during the day are a disease-specific effect. BioMed Central 2013-03-28 /pmc/articles/PMC3621557/ /pubmed/23537329 http://dx.doi.org/10.1186/2045-7022-3-8 Text en Copyright © 2013 Davidson et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Davidson, Warren J
Wong, Lisa E
The, Stephanie
Leigh, Richard
The impact of diurnal variation on induced sputum cell counts in healthy adults
title The impact of diurnal variation on induced sputum cell counts in healthy adults
title_full The impact of diurnal variation on induced sputum cell counts in healthy adults
title_fullStr The impact of diurnal variation on induced sputum cell counts in healthy adults
title_full_unstemmed The impact of diurnal variation on induced sputum cell counts in healthy adults
title_short The impact of diurnal variation on induced sputum cell counts in healthy adults
title_sort impact of diurnal variation on induced sputum cell counts in healthy adults
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3621557/
https://www.ncbi.nlm.nih.gov/pubmed/23537329
http://dx.doi.org/10.1186/2045-7022-3-8
work_keys_str_mv AT davidsonwarrenj theimpactofdiurnalvariationoninducedsputumcellcountsinhealthyadults
AT wonglisae theimpactofdiurnalvariationoninducedsputumcellcountsinhealthyadults
AT thestephanie theimpactofdiurnalvariationoninducedsputumcellcountsinhealthyadults
AT leighrichard theimpactofdiurnalvariationoninducedsputumcellcountsinhealthyadults
AT davidsonwarrenj impactofdiurnalvariationoninducedsputumcellcountsinhealthyadults
AT wonglisae impactofdiurnalvariationoninducedsputumcellcountsinhealthyadults
AT thestephanie impactofdiurnalvariationoninducedsputumcellcountsinhealthyadults
AT leighrichard impactofdiurnalvariationoninducedsputumcellcountsinhealthyadults