Cargando…
Cobalamin Analogues in Humans: A Study on Maternal and Cord Blood
BACKGROUND: Haptocorrin (HC) carries cobalamin analogues (CorA), but whether CorA are produced in the body is unknown. All cobalamins (Cbl) to the foetus are delivered by the Cbl-specific protein transcobalamin (TC), and therefore analysis of cord serum for CorA may help to clarify the origin of Cor...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3621755/ https://www.ncbi.nlm.nih.gov/pubmed/23585880 http://dx.doi.org/10.1371/journal.pone.0061194 |
_version_ | 1782265756227993600 |
---|---|
author | Hardlei, Tore Forsingdal Obeid, Rima Herrmann, Wolfgang Nexo, Ebba |
author_facet | Hardlei, Tore Forsingdal Obeid, Rima Herrmann, Wolfgang Nexo, Ebba |
author_sort | Hardlei, Tore Forsingdal |
collection | PubMed |
description | BACKGROUND: Haptocorrin (HC) carries cobalamin analogues (CorA), but whether CorA are produced in the body is unknown. All cobalamins (Cbl) to the foetus are delivered by the Cbl-specific protein transcobalamin (TC), and therefore analysis of cord serum for CorA may help to clarify the origin of CorA. METHODS: HC-CorA were quantified in paired samples of cord serum from newborns and serum from mothers (n = 69). RESULTS: The CorA-concentration was higher in cord serum (median = 380, range: 41–780 pmol/L) than in serum from the mothers (median = 160, range: 64–330 pmol/L), (p<0.0001). HPLC-analysis showed CorA-peaks with retention times of 13.5, 14,5 and 16.5 min in samples from both the mother and cord serum. The peak with retention time 16.5 min constituted 24% (mother) and 45% (cord serum) of the total amount CorA, and eluted as does dicyanocobinamide. CONCLUSION: Our results support that CorA in the human body are derived from Cbl. |
format | Online Article Text |
id | pubmed-3621755 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36217552013-04-12 Cobalamin Analogues in Humans: A Study on Maternal and Cord Blood Hardlei, Tore Forsingdal Obeid, Rima Herrmann, Wolfgang Nexo, Ebba PLoS One Research Article BACKGROUND: Haptocorrin (HC) carries cobalamin analogues (CorA), but whether CorA are produced in the body is unknown. All cobalamins (Cbl) to the foetus are delivered by the Cbl-specific protein transcobalamin (TC), and therefore analysis of cord serum for CorA may help to clarify the origin of CorA. METHODS: HC-CorA were quantified in paired samples of cord serum from newborns and serum from mothers (n = 69). RESULTS: The CorA-concentration was higher in cord serum (median = 380, range: 41–780 pmol/L) than in serum from the mothers (median = 160, range: 64–330 pmol/L), (p<0.0001). HPLC-analysis showed CorA-peaks with retention times of 13.5, 14,5 and 16.5 min in samples from both the mother and cord serum. The peak with retention time 16.5 min constituted 24% (mother) and 45% (cord serum) of the total amount CorA, and eluted as does dicyanocobinamide. CONCLUSION: Our results support that CorA in the human body are derived from Cbl. Public Library of Science 2013-04-09 /pmc/articles/PMC3621755/ /pubmed/23585880 http://dx.doi.org/10.1371/journal.pone.0061194 Text en © 2013 Hardlei et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Hardlei, Tore Forsingdal Obeid, Rima Herrmann, Wolfgang Nexo, Ebba Cobalamin Analogues in Humans: A Study on Maternal and Cord Blood |
title | Cobalamin Analogues in Humans: A Study on Maternal and Cord Blood |
title_full | Cobalamin Analogues in Humans: A Study on Maternal and Cord Blood |
title_fullStr | Cobalamin Analogues in Humans: A Study on Maternal and Cord Blood |
title_full_unstemmed | Cobalamin Analogues in Humans: A Study on Maternal and Cord Blood |
title_short | Cobalamin Analogues in Humans: A Study on Maternal and Cord Blood |
title_sort | cobalamin analogues in humans: a study on maternal and cord blood |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3621755/ https://www.ncbi.nlm.nih.gov/pubmed/23585880 http://dx.doi.org/10.1371/journal.pone.0061194 |
work_keys_str_mv | AT hardleitoreforsingdal cobalaminanaloguesinhumansastudyonmaternalandcordblood AT obeidrima cobalaminanaloguesinhumansastudyonmaternalandcordblood AT herrmannwolfgang cobalaminanaloguesinhumansastudyonmaternalandcordblood AT nexoebba cobalaminanaloguesinhumansastudyonmaternalandcordblood |