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Importance of the ammonia assimilation by Penicillium purpurogenum in amino derivative Monascus pigment, PP-V, production

A fungal strain, Penicillium purpurogenum IAM 15392, produced the azaphilone Monascus pigment homolog when cultured in a medium composed of soluble starch, ammonium nitrate, yeast extract, and citrate buffer, pH 5.0. One of the typical features of violet pigment PP-V [(10Z)-12- carboxyl-monascorubra...

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Autores principales: Arai, Teppei, Koganei, Kasumi, Umemura, Sara, Kojima, Ryo, Kato, Jun, Kasumi, Takafumi, Ogihara, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3621813/
https://www.ncbi.nlm.nih.gov/pubmed/23537394
http://dx.doi.org/10.1186/2191-0855-3-19
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author Arai, Teppei
Koganei, Kasumi
Umemura, Sara
Kojima, Ryo
Kato, Jun
Kasumi, Takafumi
Ogihara, Jun
author_facet Arai, Teppei
Koganei, Kasumi
Umemura, Sara
Kojima, Ryo
Kato, Jun
Kasumi, Takafumi
Ogihara, Jun
author_sort Arai, Teppei
collection PubMed
description A fungal strain, Penicillium purpurogenum IAM 15392, produced the azaphilone Monascus pigment homolog when cultured in a medium composed of soluble starch, ammonium nitrate, yeast extract, and citrate buffer, pH 5.0. One of the typical features of violet pigment PP-V [(10Z)-12- carboxyl-monascorubramine] is that pyranoid oxygen is replaced with nitrogen. In this study, we found that glutamine synthetase (glnB) and glutamate dehydrogenase (gdh1) genes were expressed in the culture conditions conducive to PP-V production. Gln and Glu both support PP-V biosynthesis, but PP-V biosynthesis was much more efficient with Gln. We determined that synthesis of Gln by glutamine synthetase from ammonium is important for PP-V production.
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spelling pubmed-36218132013-04-10 Importance of the ammonia assimilation by Penicillium purpurogenum in amino derivative Monascus pigment, PP-V, production Arai, Teppei Koganei, Kasumi Umemura, Sara Kojima, Ryo Kato, Jun Kasumi, Takafumi Ogihara, Jun AMB Express Original Article A fungal strain, Penicillium purpurogenum IAM 15392, produced the azaphilone Monascus pigment homolog when cultured in a medium composed of soluble starch, ammonium nitrate, yeast extract, and citrate buffer, pH 5.0. One of the typical features of violet pigment PP-V [(10Z)-12- carboxyl-monascorubramine] is that pyranoid oxygen is replaced with nitrogen. In this study, we found that glutamine synthetase (glnB) and glutamate dehydrogenase (gdh1) genes were expressed in the culture conditions conducive to PP-V production. Gln and Glu both support PP-V biosynthesis, but PP-V biosynthesis was much more efficient with Gln. We determined that synthesis of Gln by glutamine synthetase from ammonium is important for PP-V production. Springer 2013-03-28 /pmc/articles/PMC3621813/ /pubmed/23537394 http://dx.doi.org/10.1186/2191-0855-3-19 Text en Copyright ©2013 Arai et al.; licensee Springer. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Arai, Teppei
Koganei, Kasumi
Umemura, Sara
Kojima, Ryo
Kato, Jun
Kasumi, Takafumi
Ogihara, Jun
Importance of the ammonia assimilation by Penicillium purpurogenum in amino derivative Monascus pigment, PP-V, production
title Importance of the ammonia assimilation by Penicillium purpurogenum in amino derivative Monascus pigment, PP-V, production
title_full Importance of the ammonia assimilation by Penicillium purpurogenum in amino derivative Monascus pigment, PP-V, production
title_fullStr Importance of the ammonia assimilation by Penicillium purpurogenum in amino derivative Monascus pigment, PP-V, production
title_full_unstemmed Importance of the ammonia assimilation by Penicillium purpurogenum in amino derivative Monascus pigment, PP-V, production
title_short Importance of the ammonia assimilation by Penicillium purpurogenum in amino derivative Monascus pigment, PP-V, production
title_sort importance of the ammonia assimilation by penicillium purpurogenum in amino derivative monascus pigment, pp-v, production
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3621813/
https://www.ncbi.nlm.nih.gov/pubmed/23537394
http://dx.doi.org/10.1186/2191-0855-3-19
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