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Importance of the ammonia assimilation by Penicillium purpurogenum in amino derivative Monascus pigment, PP-V, production
A fungal strain, Penicillium purpurogenum IAM 15392, produced the azaphilone Monascus pigment homolog when cultured in a medium composed of soluble starch, ammonium nitrate, yeast extract, and citrate buffer, pH 5.0. One of the typical features of violet pigment PP-V [(10Z)-12- carboxyl-monascorubra...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3621813/ https://www.ncbi.nlm.nih.gov/pubmed/23537394 http://dx.doi.org/10.1186/2191-0855-3-19 |
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author | Arai, Teppei Koganei, Kasumi Umemura, Sara Kojima, Ryo Kato, Jun Kasumi, Takafumi Ogihara, Jun |
author_facet | Arai, Teppei Koganei, Kasumi Umemura, Sara Kojima, Ryo Kato, Jun Kasumi, Takafumi Ogihara, Jun |
author_sort | Arai, Teppei |
collection | PubMed |
description | A fungal strain, Penicillium purpurogenum IAM 15392, produced the azaphilone Monascus pigment homolog when cultured in a medium composed of soluble starch, ammonium nitrate, yeast extract, and citrate buffer, pH 5.0. One of the typical features of violet pigment PP-V [(10Z)-12- carboxyl-monascorubramine] is that pyranoid oxygen is replaced with nitrogen. In this study, we found that glutamine synthetase (glnB) and glutamate dehydrogenase (gdh1) genes were expressed in the culture conditions conducive to PP-V production. Gln and Glu both support PP-V biosynthesis, but PP-V biosynthesis was much more efficient with Gln. We determined that synthesis of Gln by glutamine synthetase from ammonium is important for PP-V production. |
format | Online Article Text |
id | pubmed-3621813 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Springer |
record_format | MEDLINE/PubMed |
spelling | pubmed-36218132013-04-10 Importance of the ammonia assimilation by Penicillium purpurogenum in amino derivative Monascus pigment, PP-V, production Arai, Teppei Koganei, Kasumi Umemura, Sara Kojima, Ryo Kato, Jun Kasumi, Takafumi Ogihara, Jun AMB Express Original Article A fungal strain, Penicillium purpurogenum IAM 15392, produced the azaphilone Monascus pigment homolog when cultured in a medium composed of soluble starch, ammonium nitrate, yeast extract, and citrate buffer, pH 5.0. One of the typical features of violet pigment PP-V [(10Z)-12- carboxyl-monascorubramine] is that pyranoid oxygen is replaced with nitrogen. In this study, we found that glutamine synthetase (glnB) and glutamate dehydrogenase (gdh1) genes were expressed in the culture conditions conducive to PP-V production. Gln and Glu both support PP-V biosynthesis, but PP-V biosynthesis was much more efficient with Gln. We determined that synthesis of Gln by glutamine synthetase from ammonium is important for PP-V production. Springer 2013-03-28 /pmc/articles/PMC3621813/ /pubmed/23537394 http://dx.doi.org/10.1186/2191-0855-3-19 Text en Copyright ©2013 Arai et al.; licensee Springer. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Arai, Teppei Koganei, Kasumi Umemura, Sara Kojima, Ryo Kato, Jun Kasumi, Takafumi Ogihara, Jun Importance of the ammonia assimilation by Penicillium purpurogenum in amino derivative Monascus pigment, PP-V, production |
title | Importance of the ammonia assimilation by Penicillium purpurogenum in amino derivative Monascus pigment, PP-V, production |
title_full | Importance of the ammonia assimilation by Penicillium purpurogenum in amino derivative Monascus pigment, PP-V, production |
title_fullStr | Importance of the ammonia assimilation by Penicillium purpurogenum in amino derivative Monascus pigment, PP-V, production |
title_full_unstemmed | Importance of the ammonia assimilation by Penicillium purpurogenum in amino derivative Monascus pigment, PP-V, production |
title_short | Importance of the ammonia assimilation by Penicillium purpurogenum in amino derivative Monascus pigment, PP-V, production |
title_sort | importance of the ammonia assimilation by penicillium purpurogenum in amino derivative monascus pigment, pp-v, production |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3621813/ https://www.ncbi.nlm.nih.gov/pubmed/23537394 http://dx.doi.org/10.1186/2191-0855-3-19 |
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