Cargando…

Stress-Induced Lipocalin-2 Controls Dendritic Spine Formation and Neuronal Activity in the Amygdala

Behavioural adaptation to psychological stress is dependent on neuronal plasticity and dysfunction at this cellular level may underlie the pathogenesis of affective disorders such as depression and post-traumatic stress disorder. Taking advantage of genome-wide microarray assay, we performed detaile...

Descripción completa

Detalles Bibliográficos
Autores principales: Skrzypiec, Anna E., Shah, Rahul S., Schiavon, Emanuele, Baker, Eva, Skene, Nathan, Pawlak, Robert, Mucha, Mariusz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3621903/
https://www.ncbi.nlm.nih.gov/pubmed/23593384
http://dx.doi.org/10.1371/journal.pone.0061046
_version_ 1782265784448319488
author Skrzypiec, Anna E.
Shah, Rahul S.
Schiavon, Emanuele
Baker, Eva
Skene, Nathan
Pawlak, Robert
Mucha, Mariusz
author_facet Skrzypiec, Anna E.
Shah, Rahul S.
Schiavon, Emanuele
Baker, Eva
Skene, Nathan
Pawlak, Robert
Mucha, Mariusz
author_sort Skrzypiec, Anna E.
collection PubMed
description Behavioural adaptation to psychological stress is dependent on neuronal plasticity and dysfunction at this cellular level may underlie the pathogenesis of affective disorders such as depression and post-traumatic stress disorder. Taking advantage of genome-wide microarray assay, we performed detailed studies of stress-affected transcripts in the amygdala – an area which forms part of the innate fear circuit in mammals. Having previously demonstrated the role of lipocalin-2 (Lcn-2) in promoting stress-induced changes in dendritic spine morphology/function and neuronal excitability in the mouse hippocampus, we show here that the Lcn-2 gene is one of the most highly upregulated transcripts detected by microarray analysis in the amygdala after acute restraint-induced psychological stress. This is associated with increased Lcn-2 protein synthesis, which is found on immunohistochemistry to be predominantly localised to neurons. Stress-naïve Lcn-2(−/−) mice show a higher spine density in the basolateral amygdala and a 2-fold higher rate of neuronal firing rate compared to wild-type mice. Unlike their wild-type counterparts, Lcn-2(−/−) mice did not show an increase in dendritic spine density in response to stress but did show a distinct pattern of spine morphology. Thus, amygdala-specific neuronal responses to Lcn-2 may represent a mechanism for behavioural adaptation to psychological stress.
format Online
Article
Text
id pubmed-3621903
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-36219032013-04-16 Stress-Induced Lipocalin-2 Controls Dendritic Spine Formation and Neuronal Activity in the Amygdala Skrzypiec, Anna E. Shah, Rahul S. Schiavon, Emanuele Baker, Eva Skene, Nathan Pawlak, Robert Mucha, Mariusz PLoS One Research Article Behavioural adaptation to psychological stress is dependent on neuronal plasticity and dysfunction at this cellular level may underlie the pathogenesis of affective disorders such as depression and post-traumatic stress disorder. Taking advantage of genome-wide microarray assay, we performed detailed studies of stress-affected transcripts in the amygdala – an area which forms part of the innate fear circuit in mammals. Having previously demonstrated the role of lipocalin-2 (Lcn-2) in promoting stress-induced changes in dendritic spine morphology/function and neuronal excitability in the mouse hippocampus, we show here that the Lcn-2 gene is one of the most highly upregulated transcripts detected by microarray analysis in the amygdala after acute restraint-induced psychological stress. This is associated with increased Lcn-2 protein synthesis, which is found on immunohistochemistry to be predominantly localised to neurons. Stress-naïve Lcn-2(−/−) mice show a higher spine density in the basolateral amygdala and a 2-fold higher rate of neuronal firing rate compared to wild-type mice. Unlike their wild-type counterparts, Lcn-2(−/−) mice did not show an increase in dendritic spine density in response to stress but did show a distinct pattern of spine morphology. Thus, amygdala-specific neuronal responses to Lcn-2 may represent a mechanism for behavioural adaptation to psychological stress. Public Library of Science 2013-04-09 /pmc/articles/PMC3621903/ /pubmed/23593384 http://dx.doi.org/10.1371/journal.pone.0061046 Text en © 2013 Skrzypiec et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Skrzypiec, Anna E.
Shah, Rahul S.
Schiavon, Emanuele
Baker, Eva
Skene, Nathan
Pawlak, Robert
Mucha, Mariusz
Stress-Induced Lipocalin-2 Controls Dendritic Spine Formation and Neuronal Activity in the Amygdala
title Stress-Induced Lipocalin-2 Controls Dendritic Spine Formation and Neuronal Activity in the Amygdala
title_full Stress-Induced Lipocalin-2 Controls Dendritic Spine Formation and Neuronal Activity in the Amygdala
title_fullStr Stress-Induced Lipocalin-2 Controls Dendritic Spine Formation and Neuronal Activity in the Amygdala
title_full_unstemmed Stress-Induced Lipocalin-2 Controls Dendritic Spine Formation and Neuronal Activity in the Amygdala
title_short Stress-Induced Lipocalin-2 Controls Dendritic Spine Formation and Neuronal Activity in the Amygdala
title_sort stress-induced lipocalin-2 controls dendritic spine formation and neuronal activity in the amygdala
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3621903/
https://www.ncbi.nlm.nih.gov/pubmed/23593384
http://dx.doi.org/10.1371/journal.pone.0061046
work_keys_str_mv AT skrzypiecannae stressinducedlipocalin2controlsdendriticspineformationandneuronalactivityintheamygdala
AT shahrahuls stressinducedlipocalin2controlsdendriticspineformationandneuronalactivityintheamygdala
AT schiavonemanuele stressinducedlipocalin2controlsdendriticspineformationandneuronalactivityintheamygdala
AT bakereva stressinducedlipocalin2controlsdendriticspineformationandneuronalactivityintheamygdala
AT skenenathan stressinducedlipocalin2controlsdendriticspineformationandneuronalactivityintheamygdala
AT pawlakrobert stressinducedlipocalin2controlsdendriticspineformationandneuronalactivityintheamygdala
AT muchamariusz stressinducedlipocalin2controlsdendriticspineformationandneuronalactivityintheamygdala