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Streptomyces misionensis PESB-25 Produces a Thermoacidophilic Endoglucanase Using Sugarcane Bagasse and Corn Steep Liquor as the Sole Organic Substrates
Streptomyces misionensis strain PESB-25 was screened and selected for its ability to secrete cellulases. Cells were grown in a liquid medium containing sugarcane bagasse (SCB) as carbon source and corn steep liquor (CSL) as nitrogen source, whose concentrations were optimized using response surface...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3622310/ https://www.ncbi.nlm.nih.gov/pubmed/23586048 http://dx.doi.org/10.1155/2013/584207 |
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author | Franco-Cirigliano, Marcella Novaes Rezende, Raquel de Carvalho Gravina-Oliveira, Mônica Pires Pereira, Pedro Henrique Freitas do Nascimento, Rodrigo Pires Bon, Elba Pinto da Silva Macrae, Andrew Coelho, Rosalie Reed Rodrigues |
author_facet | Franco-Cirigliano, Marcella Novaes Rezende, Raquel de Carvalho Gravina-Oliveira, Mônica Pires Pereira, Pedro Henrique Freitas do Nascimento, Rodrigo Pires Bon, Elba Pinto da Silva Macrae, Andrew Coelho, Rosalie Reed Rodrigues |
author_sort | Franco-Cirigliano, Marcella Novaes |
collection | PubMed |
description | Streptomyces misionensis strain PESB-25 was screened and selected for its ability to secrete cellulases. Cells were grown in a liquid medium containing sugarcane bagasse (SCB) as carbon source and corn steep liquor (CSL) as nitrogen source, whose concentrations were optimized using response surface methodology (RSM). A peak of endoglucanase accumulation (1.01 U·mL(−1)) was observed in a medium with SCB 1.0% (w/v) and CSL 1.2% (w/v) within three days of cultivation. S. misionensis PESB-25 endoglucanase activity was thermoacidophilic with optimum pH and temperature range of 3.0 to 3.6 and 62° to 70°C, respectively. In these conditions, values of 1.54 U mL(−1) of endoglucanase activity were observed. Moreover, Mn(2+) was demonstrated to have a hyperactivating effect on the enzyme. In the presence of MnSO(4) (8 mM), the enzyme activity increased threefold, up to 4.34 U·mL(−1). Mn(2+) also improved endoglucanase stability as the catalyst retained almost full activity upon incubation at 50°C for 4 h, while in the absence of Mn(2+), enzyme activity decreased by 50% in this same period. Three protein bands with endoglucanase activity and apparent molecular masses of 12, 48.5 and 119.5 kDa were detected by zymogram. |
format | Online Article Text |
id | pubmed-3622310 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-36223102013-04-12 Streptomyces misionensis PESB-25 Produces a Thermoacidophilic Endoglucanase Using Sugarcane Bagasse and Corn Steep Liquor as the Sole Organic Substrates Franco-Cirigliano, Marcella Novaes Rezende, Raquel de Carvalho Gravina-Oliveira, Mônica Pires Pereira, Pedro Henrique Freitas do Nascimento, Rodrigo Pires Bon, Elba Pinto da Silva Macrae, Andrew Coelho, Rosalie Reed Rodrigues Biomed Res Int Research Article Streptomyces misionensis strain PESB-25 was screened and selected for its ability to secrete cellulases. Cells were grown in a liquid medium containing sugarcane bagasse (SCB) as carbon source and corn steep liquor (CSL) as nitrogen source, whose concentrations were optimized using response surface methodology (RSM). A peak of endoglucanase accumulation (1.01 U·mL(−1)) was observed in a medium with SCB 1.0% (w/v) and CSL 1.2% (w/v) within three days of cultivation. S. misionensis PESB-25 endoglucanase activity was thermoacidophilic with optimum pH and temperature range of 3.0 to 3.6 and 62° to 70°C, respectively. In these conditions, values of 1.54 U mL(−1) of endoglucanase activity were observed. Moreover, Mn(2+) was demonstrated to have a hyperactivating effect on the enzyme. In the presence of MnSO(4) (8 mM), the enzyme activity increased threefold, up to 4.34 U·mL(−1). Mn(2+) also improved endoglucanase stability as the catalyst retained almost full activity upon incubation at 50°C for 4 h, while in the absence of Mn(2+), enzyme activity decreased by 50% in this same period. Three protein bands with endoglucanase activity and apparent molecular masses of 12, 48.5 and 119.5 kDa were detected by zymogram. Hindawi Publishing Corporation 2013 2013-03-24 /pmc/articles/PMC3622310/ /pubmed/23586048 http://dx.doi.org/10.1155/2013/584207 Text en Copyright © 2013 Marcella Novaes Franco-Cirigliano et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Franco-Cirigliano, Marcella Novaes Rezende, Raquel de Carvalho Gravina-Oliveira, Mônica Pires Pereira, Pedro Henrique Freitas do Nascimento, Rodrigo Pires Bon, Elba Pinto da Silva Macrae, Andrew Coelho, Rosalie Reed Rodrigues Streptomyces misionensis PESB-25 Produces a Thermoacidophilic Endoglucanase Using Sugarcane Bagasse and Corn Steep Liquor as the Sole Organic Substrates |
title |
Streptomyces misionensis PESB-25 Produces a Thermoacidophilic Endoglucanase Using Sugarcane Bagasse and Corn Steep Liquor as the Sole Organic Substrates |
title_full |
Streptomyces misionensis PESB-25 Produces a Thermoacidophilic Endoglucanase Using Sugarcane Bagasse and Corn Steep Liquor as the Sole Organic Substrates |
title_fullStr |
Streptomyces misionensis PESB-25 Produces a Thermoacidophilic Endoglucanase Using Sugarcane Bagasse and Corn Steep Liquor as the Sole Organic Substrates |
title_full_unstemmed |
Streptomyces misionensis PESB-25 Produces a Thermoacidophilic Endoglucanase Using Sugarcane Bagasse and Corn Steep Liquor as the Sole Organic Substrates |
title_short |
Streptomyces misionensis PESB-25 Produces a Thermoacidophilic Endoglucanase Using Sugarcane Bagasse and Corn Steep Liquor as the Sole Organic Substrates |
title_sort | streptomyces misionensis pesb-25 produces a thermoacidophilic endoglucanase using sugarcane bagasse and corn steep liquor as the sole organic substrates |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3622310/ https://www.ncbi.nlm.nih.gov/pubmed/23586048 http://dx.doi.org/10.1155/2013/584207 |
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