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Titanium Surface Coating with a Laminin-Derived Functional Peptide Promotes Bone Cell Adhesion

Laminin-derived peptide coatings can enhance epithelial cell adhesion to implants, and the positive effect of these peptides on bone cell adhesion has been anticipated. The purpose of this study was to evaluate the improvement in bone cell attachment to and activity on titanium (Ti) scaffolds coated...

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Autores principales: Min, Seung-Ki, Kang, Hyun Ki, Jang, Da Hyun, Jung, Sung Youn, Kim, O. Bok, Min, Byung-Moo, Yeo, In-Sung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3622367/
https://www.ncbi.nlm.nih.gov/pubmed/23586052
http://dx.doi.org/10.1155/2013/638348
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author Min, Seung-Ki
Kang, Hyun Ki
Jang, Da Hyun
Jung, Sung Youn
Kim, O. Bok
Min, Byung-Moo
Yeo, In-Sung
author_facet Min, Seung-Ki
Kang, Hyun Ki
Jang, Da Hyun
Jung, Sung Youn
Kim, O. Bok
Min, Byung-Moo
Yeo, In-Sung
author_sort Min, Seung-Ki
collection PubMed
description Laminin-derived peptide coatings can enhance epithelial cell adhesion to implants, and the positive effect of these peptides on bone cell adhesion has been anticipated. The purpose of this study was to evaluate the improvement in bone cell attachment to and activity on titanium (Ti) scaffolds coated with a laminin-derived functional peptide, Ln2-P3 (the DLTIDDSYWYRI motif). Four Ti disc surfaces were prepared, and a human osteosarcoma (HOS) cell attachment test was performed to select two candidate surfaces for peptide coating. These two candidates were then coated with Ln2-P3 peptide, a scrambled peptide, or left uncoated to measure cell attachment to each surface, following which one surface was chosen to assess alkaline phosphatase (ALP) activity and osteogenic marker gene expression with quantitative real-time PCR. On the commercially pure Ti surface, the Ln2-P3 coating significantly increased cellular ALP activity and the expression levels of ALP and bone sialoprotein mRNA as compared with the scrambled peptide-coated and uncoated surfaces. In conclusion, although further in vivo studies are needed, the findings of this in vitro study indicate that the Ln2-P3-coated implant surface promotes bone cell adhesion, which has clinical implications for reducing the overall treatment time of dental implant therapy.
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spelling pubmed-36223672013-04-12 Titanium Surface Coating with a Laminin-Derived Functional Peptide Promotes Bone Cell Adhesion Min, Seung-Ki Kang, Hyun Ki Jang, Da Hyun Jung, Sung Youn Kim, O. Bok Min, Byung-Moo Yeo, In-Sung Biomed Res Int Research Article Laminin-derived peptide coatings can enhance epithelial cell adhesion to implants, and the positive effect of these peptides on bone cell adhesion has been anticipated. The purpose of this study was to evaluate the improvement in bone cell attachment to and activity on titanium (Ti) scaffolds coated with a laminin-derived functional peptide, Ln2-P3 (the DLTIDDSYWYRI motif). Four Ti disc surfaces were prepared, and a human osteosarcoma (HOS) cell attachment test was performed to select two candidate surfaces for peptide coating. These two candidates were then coated with Ln2-P3 peptide, a scrambled peptide, or left uncoated to measure cell attachment to each surface, following which one surface was chosen to assess alkaline phosphatase (ALP) activity and osteogenic marker gene expression with quantitative real-time PCR. On the commercially pure Ti surface, the Ln2-P3 coating significantly increased cellular ALP activity and the expression levels of ALP and bone sialoprotein mRNA as compared with the scrambled peptide-coated and uncoated surfaces. In conclusion, although further in vivo studies are needed, the findings of this in vitro study indicate that the Ln2-P3-coated implant surface promotes bone cell adhesion, which has clinical implications for reducing the overall treatment time of dental implant therapy. Hindawi Publishing Corporation 2013 2013-03-25 /pmc/articles/PMC3622367/ /pubmed/23586052 http://dx.doi.org/10.1155/2013/638348 Text en Copyright © 2013 Seung-Ki Min et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Min, Seung-Ki
Kang, Hyun Ki
Jang, Da Hyun
Jung, Sung Youn
Kim, O. Bok
Min, Byung-Moo
Yeo, In-Sung
Titanium Surface Coating with a Laminin-Derived Functional Peptide Promotes Bone Cell Adhesion
title Titanium Surface Coating with a Laminin-Derived Functional Peptide Promotes Bone Cell Adhesion
title_full Titanium Surface Coating with a Laminin-Derived Functional Peptide Promotes Bone Cell Adhesion
title_fullStr Titanium Surface Coating with a Laminin-Derived Functional Peptide Promotes Bone Cell Adhesion
title_full_unstemmed Titanium Surface Coating with a Laminin-Derived Functional Peptide Promotes Bone Cell Adhesion
title_short Titanium Surface Coating with a Laminin-Derived Functional Peptide Promotes Bone Cell Adhesion
title_sort titanium surface coating with a laminin-derived functional peptide promotes bone cell adhesion
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3622367/
https://www.ncbi.nlm.nih.gov/pubmed/23586052
http://dx.doi.org/10.1155/2013/638348
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