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Titanium Surface Coating with a Laminin-Derived Functional Peptide Promotes Bone Cell Adhesion
Laminin-derived peptide coatings can enhance epithelial cell adhesion to implants, and the positive effect of these peptides on bone cell adhesion has been anticipated. The purpose of this study was to evaluate the improvement in bone cell attachment to and activity on titanium (Ti) scaffolds coated...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3622367/ https://www.ncbi.nlm.nih.gov/pubmed/23586052 http://dx.doi.org/10.1155/2013/638348 |
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author | Min, Seung-Ki Kang, Hyun Ki Jang, Da Hyun Jung, Sung Youn Kim, O. Bok Min, Byung-Moo Yeo, In-Sung |
author_facet | Min, Seung-Ki Kang, Hyun Ki Jang, Da Hyun Jung, Sung Youn Kim, O. Bok Min, Byung-Moo Yeo, In-Sung |
author_sort | Min, Seung-Ki |
collection | PubMed |
description | Laminin-derived peptide coatings can enhance epithelial cell adhesion to implants, and the positive effect of these peptides on bone cell adhesion has been anticipated. The purpose of this study was to evaluate the improvement in bone cell attachment to and activity on titanium (Ti) scaffolds coated with a laminin-derived functional peptide, Ln2-P3 (the DLTIDDSYWYRI motif). Four Ti disc surfaces were prepared, and a human osteosarcoma (HOS) cell attachment test was performed to select two candidate surfaces for peptide coating. These two candidates were then coated with Ln2-P3 peptide, a scrambled peptide, or left uncoated to measure cell attachment to each surface, following which one surface was chosen to assess alkaline phosphatase (ALP) activity and osteogenic marker gene expression with quantitative real-time PCR. On the commercially pure Ti surface, the Ln2-P3 coating significantly increased cellular ALP activity and the expression levels of ALP and bone sialoprotein mRNA as compared with the scrambled peptide-coated and uncoated surfaces. In conclusion, although further in vivo studies are needed, the findings of this in vitro study indicate that the Ln2-P3-coated implant surface promotes bone cell adhesion, which has clinical implications for reducing the overall treatment time of dental implant therapy. |
format | Online Article Text |
id | pubmed-3622367 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-36223672013-04-12 Titanium Surface Coating with a Laminin-Derived Functional Peptide Promotes Bone Cell Adhesion Min, Seung-Ki Kang, Hyun Ki Jang, Da Hyun Jung, Sung Youn Kim, O. Bok Min, Byung-Moo Yeo, In-Sung Biomed Res Int Research Article Laminin-derived peptide coatings can enhance epithelial cell adhesion to implants, and the positive effect of these peptides on bone cell adhesion has been anticipated. The purpose of this study was to evaluate the improvement in bone cell attachment to and activity on titanium (Ti) scaffolds coated with a laminin-derived functional peptide, Ln2-P3 (the DLTIDDSYWYRI motif). Four Ti disc surfaces were prepared, and a human osteosarcoma (HOS) cell attachment test was performed to select two candidate surfaces for peptide coating. These two candidates were then coated with Ln2-P3 peptide, a scrambled peptide, or left uncoated to measure cell attachment to each surface, following which one surface was chosen to assess alkaline phosphatase (ALP) activity and osteogenic marker gene expression with quantitative real-time PCR. On the commercially pure Ti surface, the Ln2-P3 coating significantly increased cellular ALP activity and the expression levels of ALP and bone sialoprotein mRNA as compared with the scrambled peptide-coated and uncoated surfaces. In conclusion, although further in vivo studies are needed, the findings of this in vitro study indicate that the Ln2-P3-coated implant surface promotes bone cell adhesion, which has clinical implications for reducing the overall treatment time of dental implant therapy. Hindawi Publishing Corporation 2013 2013-03-25 /pmc/articles/PMC3622367/ /pubmed/23586052 http://dx.doi.org/10.1155/2013/638348 Text en Copyright © 2013 Seung-Ki Min et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Min, Seung-Ki Kang, Hyun Ki Jang, Da Hyun Jung, Sung Youn Kim, O. Bok Min, Byung-Moo Yeo, In-Sung Titanium Surface Coating with a Laminin-Derived Functional Peptide Promotes Bone Cell Adhesion |
title | Titanium Surface Coating with a Laminin-Derived Functional Peptide Promotes Bone Cell Adhesion |
title_full | Titanium Surface Coating with a Laminin-Derived Functional Peptide Promotes Bone Cell Adhesion |
title_fullStr | Titanium Surface Coating with a Laminin-Derived Functional Peptide Promotes Bone Cell Adhesion |
title_full_unstemmed | Titanium Surface Coating with a Laminin-Derived Functional Peptide Promotes Bone Cell Adhesion |
title_short | Titanium Surface Coating with a Laminin-Derived Functional Peptide Promotes Bone Cell Adhesion |
title_sort | titanium surface coating with a laminin-derived functional peptide promotes bone cell adhesion |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3622367/ https://www.ncbi.nlm.nih.gov/pubmed/23586052 http://dx.doi.org/10.1155/2013/638348 |
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