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Fluid-Flow Induced Wall Shear Stress and Epithelial Ovarian Cancer Peritoneal Spreading

Epithelial ovarian cancer (EOC) is usually discovered after extensive metastasis have developed in the peritoneal cavity. The ovarian surface is exposed to peritoneal fluid pressures and shear forces due to the continuous peristaltic motions of the gastro-intestinal system, creating a mechanical mic...

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Autores principales: Avraham-Chakim, Liron, Elad, David, Zaretsky, Uri, Kloog, Yoel, Jaffa, Ariel, Grisaru, Dan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3622607/
https://www.ncbi.nlm.nih.gov/pubmed/23593358
http://dx.doi.org/10.1371/journal.pone.0060965
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author Avraham-Chakim, Liron
Elad, David
Zaretsky, Uri
Kloog, Yoel
Jaffa, Ariel
Grisaru, Dan
author_facet Avraham-Chakim, Liron
Elad, David
Zaretsky, Uri
Kloog, Yoel
Jaffa, Ariel
Grisaru, Dan
author_sort Avraham-Chakim, Liron
collection PubMed
description Epithelial ovarian cancer (EOC) is usually discovered after extensive metastasis have developed in the peritoneal cavity. The ovarian surface is exposed to peritoneal fluid pressures and shear forces due to the continuous peristaltic motions of the gastro-intestinal system, creating a mechanical micro-environment for the cells. An in vitro experimental model was developed to expose EOC cells to steady fluid flow induced wall shear stresses (WSS). The EOC cells were cultured from OVCAR-3 cell line on denuded amniotic membranes in special wells. Wall shear stresses of 0.5, 1.0 and 1.5 dyne/cm(2) were applied on the surface of the cells under conditions that mimic the physiological environment, followed by fluorescent stains of actin and β-tubulin fibers. The cytoskeleton response to WSS included cell elongation, stress fibers formation and generation of microtubules. More cytoskeletal components were produced by the cells and arranged in a denser and more organized structure within the cytoplasm. This suggests that WSS may have a significant role in the mechanical regulation of EOC peritoneal spreading.
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spelling pubmed-36226072013-04-16 Fluid-Flow Induced Wall Shear Stress and Epithelial Ovarian Cancer Peritoneal Spreading Avraham-Chakim, Liron Elad, David Zaretsky, Uri Kloog, Yoel Jaffa, Ariel Grisaru, Dan PLoS One Research Article Epithelial ovarian cancer (EOC) is usually discovered after extensive metastasis have developed in the peritoneal cavity. The ovarian surface is exposed to peritoneal fluid pressures and shear forces due to the continuous peristaltic motions of the gastro-intestinal system, creating a mechanical micro-environment for the cells. An in vitro experimental model was developed to expose EOC cells to steady fluid flow induced wall shear stresses (WSS). The EOC cells were cultured from OVCAR-3 cell line on denuded amniotic membranes in special wells. Wall shear stresses of 0.5, 1.0 and 1.5 dyne/cm(2) were applied on the surface of the cells under conditions that mimic the physiological environment, followed by fluorescent stains of actin and β-tubulin fibers. The cytoskeleton response to WSS included cell elongation, stress fibers formation and generation of microtubules. More cytoskeletal components were produced by the cells and arranged in a denser and more organized structure within the cytoplasm. This suggests that WSS may have a significant role in the mechanical regulation of EOC peritoneal spreading. Public Library of Science 2013-04-10 /pmc/articles/PMC3622607/ /pubmed/23593358 http://dx.doi.org/10.1371/journal.pone.0060965 Text en © 2013 Avraham-Chakim et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Avraham-Chakim, Liron
Elad, David
Zaretsky, Uri
Kloog, Yoel
Jaffa, Ariel
Grisaru, Dan
Fluid-Flow Induced Wall Shear Stress and Epithelial Ovarian Cancer Peritoneal Spreading
title Fluid-Flow Induced Wall Shear Stress and Epithelial Ovarian Cancer Peritoneal Spreading
title_full Fluid-Flow Induced Wall Shear Stress and Epithelial Ovarian Cancer Peritoneal Spreading
title_fullStr Fluid-Flow Induced Wall Shear Stress and Epithelial Ovarian Cancer Peritoneal Spreading
title_full_unstemmed Fluid-Flow Induced Wall Shear Stress and Epithelial Ovarian Cancer Peritoneal Spreading
title_short Fluid-Flow Induced Wall Shear Stress and Epithelial Ovarian Cancer Peritoneal Spreading
title_sort fluid-flow induced wall shear stress and epithelial ovarian cancer peritoneal spreading
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3622607/
https://www.ncbi.nlm.nih.gov/pubmed/23593358
http://dx.doi.org/10.1371/journal.pone.0060965
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