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Prospective assessment of the gastroesophageal microbiome in VLBW neonates
BACKGROUND: The distal GI microbiota of hospitalized preterm neonates has been established to be unique from that of healthy full-term infants; the proximal GI, more specifically gastroesophageal colonization has not been systematically addressed. We prospectively evaluated early colonization of gas...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3623619/ https://www.ncbi.nlm.nih.gov/pubmed/23560555 http://dx.doi.org/10.1186/1471-2431-13-49 |
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author | Milisavljevic, Vladana Garg, Meena Vuletic, Ivan Miller, Jeff F Kim, Lauren Cunningham, Tina D Schröder, Imke |
author_facet | Milisavljevic, Vladana Garg, Meena Vuletic, Ivan Miller, Jeff F Kim, Lauren Cunningham, Tina D Schröder, Imke |
author_sort | Milisavljevic, Vladana |
collection | PubMed |
description | BACKGROUND: The distal GI microbiota of hospitalized preterm neonates has been established to be unique from that of healthy full-term infants; the proximal GI, more specifically gastroesophageal colonization has not been systematically addressed. We prospectively evaluated early colonization of gastroesophageal portion of the GI tract of VLBW infants. METHODS: This study involved 12 infants admitted to a level III NICU with gestational age (GA) 27 +/− 0.5 weeks and birth weight 1105 +/− 77 grams. The gastroesophageal microbial flora was evaluated using 16S rDNA analysis of aspirates collected in a sterile manner during the first 28 days of life. RESULTS: Bacteria were detected in 9 of the 12 neonates. Ureaplasma was the dominant species in the first week of life, however, staphylococci were the predominant bacteria overall. By the fourth week, Gram (−) bacteria increased in abundance to account for 50% of the total organisms. Firmicutes were present in the majority of the neonates and persisted throughout the 4 weeks comprising nearly half of the sequenced clones. Noticeably, only two distinct species of Staphylococcus epidermidis were found, suggesting acquisition from the environment. CONCLUSIONS: In our neonates, the esophagus and stomach environment changed from being relatively sterile at birth to becoming colonized by a phylogenetically diverse microbiota of low individual complexity. By the fourth week, we found predominance of Firmicutes and Proteobacteria. Bacteria from both phyla (CONS and Gram (−) organisms) are strongly implicated as causes of hospital-acquired infections (HAI). Evaluation of the measures preventing colonization with potentially pathogenic and pathogenic microorganisms from the hospital environment may be warranted and may suggest novel approaches to improving quality in neonatal care. |
format | Online Article Text |
id | pubmed-3623619 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-36236192013-04-12 Prospective assessment of the gastroesophageal microbiome in VLBW neonates Milisavljevic, Vladana Garg, Meena Vuletic, Ivan Miller, Jeff F Kim, Lauren Cunningham, Tina D Schröder, Imke BMC Pediatr Research Article BACKGROUND: The distal GI microbiota of hospitalized preterm neonates has been established to be unique from that of healthy full-term infants; the proximal GI, more specifically gastroesophageal colonization has not been systematically addressed. We prospectively evaluated early colonization of gastroesophageal portion of the GI tract of VLBW infants. METHODS: This study involved 12 infants admitted to a level III NICU with gestational age (GA) 27 +/− 0.5 weeks and birth weight 1105 +/− 77 grams. The gastroesophageal microbial flora was evaluated using 16S rDNA analysis of aspirates collected in a sterile manner during the first 28 days of life. RESULTS: Bacteria were detected in 9 of the 12 neonates. Ureaplasma was the dominant species in the first week of life, however, staphylococci were the predominant bacteria overall. By the fourth week, Gram (−) bacteria increased in abundance to account for 50% of the total organisms. Firmicutes were present in the majority of the neonates and persisted throughout the 4 weeks comprising nearly half of the sequenced clones. Noticeably, only two distinct species of Staphylococcus epidermidis were found, suggesting acquisition from the environment. CONCLUSIONS: In our neonates, the esophagus and stomach environment changed from being relatively sterile at birth to becoming colonized by a phylogenetically diverse microbiota of low individual complexity. By the fourth week, we found predominance of Firmicutes and Proteobacteria. Bacteria from both phyla (CONS and Gram (−) organisms) are strongly implicated as causes of hospital-acquired infections (HAI). Evaluation of the measures preventing colonization with potentially pathogenic and pathogenic microorganisms from the hospital environment may be warranted and may suggest novel approaches to improving quality in neonatal care. BioMed Central 2013-04-05 /pmc/articles/PMC3623619/ /pubmed/23560555 http://dx.doi.org/10.1186/1471-2431-13-49 Text en Copyright © 2013 Milisavljevic et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Milisavljevic, Vladana Garg, Meena Vuletic, Ivan Miller, Jeff F Kim, Lauren Cunningham, Tina D Schröder, Imke Prospective assessment of the gastroesophageal microbiome in VLBW neonates |
title | Prospective assessment of the gastroesophageal microbiome in VLBW neonates |
title_full | Prospective assessment of the gastroesophageal microbiome in VLBW neonates |
title_fullStr | Prospective assessment of the gastroesophageal microbiome in VLBW neonates |
title_full_unstemmed | Prospective assessment of the gastroesophageal microbiome in VLBW neonates |
title_short | Prospective assessment of the gastroesophageal microbiome in VLBW neonates |
title_sort | prospective assessment of the gastroesophageal microbiome in vlbw neonates |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3623619/ https://www.ncbi.nlm.nih.gov/pubmed/23560555 http://dx.doi.org/10.1186/1471-2431-13-49 |
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