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A new unique form of microRNA from human heart, microRNA-499c, promotes myofibril formation and rescues cardiac development in mutant axolotl embryos

BACKGROUND: A recessive mutation “c” in the Mexican axolotl, Ambystoma mexicanum, results in the failure of normal heart development. In homozygous recessive embryos, the hearts do not have organized myofibrils and fail to beat. In our previous studies, we identified a noncoding Myofibril-Inducing R...

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Autores principales: Kochegarov, Andrei, Moses, Ashley, Lian, William, Meyer, Jessica, Hanna, Michael C, Lemanski, Larry F
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3623845/
https://www.ncbi.nlm.nih.gov/pubmed/23522091
http://dx.doi.org/10.1186/1423-0127-20-20
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author Kochegarov, Andrei
Moses, Ashley
Lian, William
Meyer, Jessica
Hanna, Michael C
Lemanski, Larry F
author_facet Kochegarov, Andrei
Moses, Ashley
Lian, William
Meyer, Jessica
Hanna, Michael C
Lemanski, Larry F
author_sort Kochegarov, Andrei
collection PubMed
description BACKGROUND: A recessive mutation “c” in the Mexican axolotl, Ambystoma mexicanum, results in the failure of normal heart development. In homozygous recessive embryos, the hearts do not have organized myofibrils and fail to beat. In our previous studies, we identified a noncoding Myofibril-Inducing RNA (MIR) from axolotls which promotes myofibril formation and rescues heart development. RESULTS: We randomly cloned RNAs from fetal human heart. RNA from clone #291 promoted myofibril formation and induced heart development of mutant axolotls in organ culture. This RNA induced expression of cardiac markers in mutant hearts: tropomyosin, troponin and α-syntrophin. This cloned RNA matches in partial sequence alignment to human microRNA-499a and b, although it differs in length. We have concluded that this cloned RNA is unique in its length, but is still related to the microRNA-499 family. We have named this unique RNA, microRNA-499c. Thus, we will refer to this RNA derived from clone #291 as microRNA-499c throughout the rest of the paper. CONCLUSIONS: This new form, microRNA-499c, plays an important role in cardiac development.
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spelling pubmed-36238452013-04-12 A new unique form of microRNA from human heart, microRNA-499c, promotes myofibril formation and rescues cardiac development in mutant axolotl embryos Kochegarov, Andrei Moses, Ashley Lian, William Meyer, Jessica Hanna, Michael C Lemanski, Larry F J Biomed Sci Research BACKGROUND: A recessive mutation “c” in the Mexican axolotl, Ambystoma mexicanum, results in the failure of normal heart development. In homozygous recessive embryos, the hearts do not have organized myofibrils and fail to beat. In our previous studies, we identified a noncoding Myofibril-Inducing RNA (MIR) from axolotls which promotes myofibril formation and rescues heart development. RESULTS: We randomly cloned RNAs from fetal human heart. RNA from clone #291 promoted myofibril formation and induced heart development of mutant axolotls in organ culture. This RNA induced expression of cardiac markers in mutant hearts: tropomyosin, troponin and α-syntrophin. This cloned RNA matches in partial sequence alignment to human microRNA-499a and b, although it differs in length. We have concluded that this cloned RNA is unique in its length, but is still related to the microRNA-499 family. We have named this unique RNA, microRNA-499c. Thus, we will refer to this RNA derived from clone #291 as microRNA-499c throughout the rest of the paper. CONCLUSIONS: This new form, microRNA-499c, plays an important role in cardiac development. BioMed Central 2013-03-23 /pmc/articles/PMC3623845/ /pubmed/23522091 http://dx.doi.org/10.1186/1423-0127-20-20 Text en Copyright © 2013 Kochegarov et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Kochegarov, Andrei
Moses, Ashley
Lian, William
Meyer, Jessica
Hanna, Michael C
Lemanski, Larry F
A new unique form of microRNA from human heart, microRNA-499c, promotes myofibril formation and rescues cardiac development in mutant axolotl embryos
title A new unique form of microRNA from human heart, microRNA-499c, promotes myofibril formation and rescues cardiac development in mutant axolotl embryos
title_full A new unique form of microRNA from human heart, microRNA-499c, promotes myofibril formation and rescues cardiac development in mutant axolotl embryos
title_fullStr A new unique form of microRNA from human heart, microRNA-499c, promotes myofibril formation and rescues cardiac development in mutant axolotl embryos
title_full_unstemmed A new unique form of microRNA from human heart, microRNA-499c, promotes myofibril formation and rescues cardiac development in mutant axolotl embryos
title_short A new unique form of microRNA from human heart, microRNA-499c, promotes myofibril formation and rescues cardiac development in mutant axolotl embryos
title_sort new unique form of microrna from human heart, microrna-499c, promotes myofibril formation and rescues cardiac development in mutant axolotl embryos
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3623845/
https://www.ncbi.nlm.nih.gov/pubmed/23522091
http://dx.doi.org/10.1186/1423-0127-20-20
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