Cargando…

Analysis of RNA base modification and structural rearrangement by single-molecule real-time detection of reverse transcription

BACKGROUND: Zero-mode waveguides (ZMWs) are photonic nanostructures that create highly confined optical observation volumes, thereby allowing single-molecule-resolved biophysical studies at relatively high concentrations of fluorescent molecules. This principle has been successfully applied in singl...

Descripción completa

Detalles Bibliográficos
Autores principales:	Vilfan, Igor D, Tsai, Yu-Chih, Clark, Tyson A, Wegener, Jeffrey, Dai, Qing, Yi, Chengqi, Pan, Tao, Turner, Stephen W, Korlach, Jonas
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2013
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3623877/ https://www.ncbi.nlm.nih.gov/pubmed/23552456 http://dx.doi.org/10.1186/1477-3155-11-8

Ejemplares similares

Enhanced 5-methylcytosine detection in single-molecule, real-time sequencing via Tet1 oxidation
por: Clark, Tyson A, et al.
Publicado: (2013)

The birth of the Epitranscriptome: deciphering the function of RNA modifications
por: Saletore, Yogesh, et al.
Publicado: (2012)

Direct detection of DNA methylation during single-molecule, real-time sequencing
por: Flusberg, Benjamin A., et al.
Publicado: (2010)

Reversible Positioning of Single Molecules inside Zero-Mode Waveguides
por: Larkin, Joseph, et al.
Publicado: (2014)

Characterization of DNA methyltransferase specificities using single-molecule, real-time DNA sequencing
por: Clark, Tyson A., et al.
Publicado: (2012)

Characterizing modifications to a comparative effectiveness research study: the OPTIMIZE trial—using the Framework for Reporting Adaptations and Modifications to Evidence-based Interventions (FRAME)
por: Fritz, Julie M., et al.
Publicado: (2023)

A fast algorithm for the multiple genome rearrangement problem with weighted reversals and transpositions
por: Bader, Martin, et al.
Publicado: (2008)

Development of a method for evaluating the mRNA transcription activity of influenza virus RNA-dependent RNA polymerase through real-time reverse transcription polymerase chain reaction
por: Horio, Yuka, et al.
Publicado: (2021)

Development of one-step TaqMan(®) real-time reverse transcription-PCR and conventional reverse transcription-PCR assays for the detection of equine rhinitis A and B viruses
por: Lu, Zhengchun, et al.
Publicado: (2012)

Development of real-time and lateral flow strip reverse transcription recombinase polymerase Amplification assays for rapid detection of peste des petits ruminants virus
por: Yang, Yang, et al.
Publicado: (2017)

Standardized positive controls for detection of norovirus by reverse transcription PCR
por: Lee, Sung-Geun, et al.
Publicado: (2011)

Direct Detection and Sequencing of Damaged DNA Bases
por: Clark, Tyson A, et al.
Publicado: (2011)

Rapid and sensitive detection of canine distemper virus by real-time reverse transcription recombinase polymerase amplification
por: Wang, Jianchang, et al.
Publicado: (2017)

Detecting DNA Modifications from SMRT Sequencing Data by Modeling Sequence Context Dependence of Polymerase Kinetic
por: Feng, Zhixing, et al.
Publicado: (2013)

Development of one-step quantitative reverse transcription PCR for the rapid detection of flaviviruses
por: Patel, Pranav, et al.
Publicado: (2013)

Reverse protection assay: a tool to analyze transcriptional rates from individual promoters
por: Zubo, Yan O, et al.
Publicado: (2011)

Rapid detection of wheat yellow mosaic virus by reverse transcription loop-mediated isothermal amplification
por: Zhang, Zong-Ying, et al.
Publicado: (2011)

Reverse transcription recombinase-aided amplification assay for H5 subtype avian influenza virus
por: Wang, Suchun, et al.
Publicado: (2022)

EPIGENE: genome-wide transcription unit annotation using a multivariate probabilistic model of histone modifications
por: Sahu, Anshupa, et al.
Publicado: (2020)

One-step real-time multiplex reverse transcription-polymerase chain reaction assay with melt curve analysis for detection of potato leafroll virus, potato virus S, potato virus X, and potato virus Y
por: Onozuka, Nobuya, et al.
Publicado: (2021)

Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR
por: Kleiboeker, Steven B
Publicado: (2005)

Simultaneous detection and differentiation of three genotypes of Brassica yellows virus by multiplex reverse transcription-polymerase chain reaction
por: Zhang, Xiaoyan, et al.
Publicado: (2016)

Visual detection of H3 subtype avian influenza viruses by reverse transcription loop-mediated isothermal amplification assay
por: Peng, Yi, et al.
Publicado: (2011)

One-step detection of Bean pod mottle virus in soybean seeds by the reverse-transcription loop-mediated isothermal amplification
por: Wei, Qi-Wei, et al.
Publicado: (2012)

Detection of clonal antigen receptor gene rearrangement in dogs with lymphoma by real-time polymerase chain reaction and melting curve analysis
por: Langner, Kathrin FA, et al.
Publicado: (2014)

Genetic Stabilization of the Drug-Resistant PMEN1 Pneumococcus Lineage by Its Distinctive DpnIII Restriction-Modification System
por: Eutsey, Rory A., et al.
Publicado: (2015)

The Molecule Cloud - compact visualization of large collections of molecules
por: Ertl, Peter, et al.
Publicado: (2012)

Resolving the Complexity of Human Skin Metagenomes Using Single-Molecule Sequencing
por: Tsai, Yu-Chih, et al.
Publicado: (2016)

Rapid and sensitive detection of canine distemper virus by one-tube reverse transcription-insulated isothermal polymerase chain reaction
por: Wilkes, Rebecca P, et al.
Publicado: (2014)

Rapid point-of-care detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)
por: Mautner, Lena, et al.
Publicado: (2020)

Development of a reverse transcription droplet digital PCR (RT-ddPCR) assay for sensitive detection of simian immunodeficiency virus (SIV)
por: Long, Samuel, et al.
Publicado: (2021)

Inference of transcription modification in long-live yeast strains from their expression profiles
por: Cheng, Chao, et al.
Publicado: (2007)

A reverse-engineering approach to dissect post-translational modulators of transcription factor’s activity from transcriptional data
por: Gambardella, Gennaro, et al.
Publicado: (2015)

Analysis of a macrophage carbamylated proteome reveals a function in post-translational modification crosstalk
por: You, Youngki, et al.
Publicado: (2023)

Evolutional selection of a combinatorial phage library displaying randomly-rearranged various single domains of immunoglobulin (Ig)-binding proteins (IBPs) with four kinds of Ig molecules
por: Yang, Hua, et al.
Publicado: (2008)

A new reverse transcription-polymerase chain reaction method for accurate quantification
por: Shiao, Yih-Horng
Publicado: (2003)

Structural and functional diversity among Type III restriction-modification systems that confer host DNA protection via methylation of the N4 atom of cytosine
por: Murray, Iain A., et al.
Publicado: (2021)

A New Microsphere-Based Immunoassay for Measuring the Activity of Transcription Factors
por: Lin, Yu-Ling, et al.
Publicado: (2010)

A modification to geographically weighted regression
por: Leong, Yin-Yee, et al.
Publicado: (2017)

Forward genetic screen of human transposase genomic rearrangements
por: Henssen, Anton G., et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_vmec260ou8imm1krs4sktue21g