Cargando…

Genetic Diversity and Distribution of the Ciguatera-Causing Dinoflagellate Gambierdiscus spp. (Dinophyceae) in Coastal Areas of Japan

BACKGROUND: The marine epiphytic dinoflagellate genus Gambierdiscus produce toxins that cause ciguatera fish poisoning (CFP): one of the most significant seafood-borne illnesses associated with fish consumption worldwide. So far, occurrences of CFP incidents in Japan have been mainly reported in sub...

Descripción completa

Detalles Bibliográficos
Autores principales: Nishimura, Tomohiro, Sato, Shinya, Tawong, Wittaya, Sakanari, Hiroshi, Uehara, Keita, Shah, Md Mahfuzur Rahman, Suda, Shoichiro, Yasumoto, Takeshi, Taira, Yohsuke, Yamaguchi, Haruo, Adachi, Masao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3623954/
https://www.ncbi.nlm.nih.gov/pubmed/23593339
http://dx.doi.org/10.1371/journal.pone.0060882
_version_ 1782265999662252032
author Nishimura, Tomohiro
Sato, Shinya
Tawong, Wittaya
Sakanari, Hiroshi
Uehara, Keita
Shah, Md Mahfuzur Rahman
Suda, Shoichiro
Yasumoto, Takeshi
Taira, Yohsuke
Yamaguchi, Haruo
Adachi, Masao
author_facet Nishimura, Tomohiro
Sato, Shinya
Tawong, Wittaya
Sakanari, Hiroshi
Uehara, Keita
Shah, Md Mahfuzur Rahman
Suda, Shoichiro
Yasumoto, Takeshi
Taira, Yohsuke
Yamaguchi, Haruo
Adachi, Masao
author_sort Nishimura, Tomohiro
collection PubMed
description BACKGROUND: The marine epiphytic dinoflagellate genus Gambierdiscus produce toxins that cause ciguatera fish poisoning (CFP): one of the most significant seafood-borne illnesses associated with fish consumption worldwide. So far, occurrences of CFP incidents in Japan have been mainly reported in subtropical areas. A previous phylogeographic study of Japanese Gambierdiscus revealed the existence of two distinct phylotypes: Gambierdiscus sp. type 1 from subtropical and Gambierdiscus sp. type 2 from temperate areas. However, details of the genetic diversity and distribution for Japanese Gambierdiscus are still unclear, because a comprehensive investigation has not been conducted yet. METHODS/PRINCIPAL FINDING: A total of 248 strains were examined from samples mainly collected from western and southern coastal areas of Japan during 2006–2011. The SSU rDNA, the LSU rDNA D8–D10 and the ITS region were selected as genetic markers and phylogenetic analyses were conducted. The genetic diversity of Japanese Gambierdiscus was high since five species/phylotypes were detected: including two reported phylotypes (Gambierdiscus sp. type 1 and Gambierdiscus sp. type 2), two species of Gambierdiscus (G. australes and G. cf. yasumotoi) and a hitherto unreported phylotype Gambierdiscus sp. type 3. The distributions of type 3 and G. cf. yasumotoi were restricted to the temperate and the subtropical area, respectively. On the other hand, type 1, type 2 and G. australes occurred from the subtropical to the temperate area, with a tendency that type 1 and G. australes were dominant in the subtropical area, whereas type 2 was dominant in the temperate area. By using mouse bioassay, type 1, type 3 and G. australes exhibited mouse toxicities. CONCLUSIONS/SIGNIFICANCE: This study revealed a surprising diversity of Japanese Gambierdiscus and the distribution of five species/phylotypes displayed clear geographical patterns in Japanese coastal areas. The SSU rDNA and the LSU rDNA D8–D10 as genetic markers are recommended for further use.
format Online
Article
Text
id pubmed-3623954
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-36239542013-04-16 Genetic Diversity and Distribution of the Ciguatera-Causing Dinoflagellate Gambierdiscus spp. (Dinophyceae) in Coastal Areas of Japan Nishimura, Tomohiro Sato, Shinya Tawong, Wittaya Sakanari, Hiroshi Uehara, Keita Shah, Md Mahfuzur Rahman Suda, Shoichiro Yasumoto, Takeshi Taira, Yohsuke Yamaguchi, Haruo Adachi, Masao PLoS One Research Article BACKGROUND: The marine epiphytic dinoflagellate genus Gambierdiscus produce toxins that cause ciguatera fish poisoning (CFP): one of the most significant seafood-borne illnesses associated with fish consumption worldwide. So far, occurrences of CFP incidents in Japan have been mainly reported in subtropical areas. A previous phylogeographic study of Japanese Gambierdiscus revealed the existence of two distinct phylotypes: Gambierdiscus sp. type 1 from subtropical and Gambierdiscus sp. type 2 from temperate areas. However, details of the genetic diversity and distribution for Japanese Gambierdiscus are still unclear, because a comprehensive investigation has not been conducted yet. METHODS/PRINCIPAL FINDING: A total of 248 strains were examined from samples mainly collected from western and southern coastal areas of Japan during 2006–2011. The SSU rDNA, the LSU rDNA D8–D10 and the ITS region were selected as genetic markers and phylogenetic analyses were conducted. The genetic diversity of Japanese Gambierdiscus was high since five species/phylotypes were detected: including two reported phylotypes (Gambierdiscus sp. type 1 and Gambierdiscus sp. type 2), two species of Gambierdiscus (G. australes and G. cf. yasumotoi) and a hitherto unreported phylotype Gambierdiscus sp. type 3. The distributions of type 3 and G. cf. yasumotoi were restricted to the temperate and the subtropical area, respectively. On the other hand, type 1, type 2 and G. australes occurred from the subtropical to the temperate area, with a tendency that type 1 and G. australes were dominant in the subtropical area, whereas type 2 was dominant in the temperate area. By using mouse bioassay, type 1, type 3 and G. australes exhibited mouse toxicities. CONCLUSIONS/SIGNIFICANCE: This study revealed a surprising diversity of Japanese Gambierdiscus and the distribution of five species/phylotypes displayed clear geographical patterns in Japanese coastal areas. The SSU rDNA and the LSU rDNA D8–D10 as genetic markers are recommended for further use. Public Library of Science 2013-04-11 /pmc/articles/PMC3623954/ /pubmed/23593339 http://dx.doi.org/10.1371/journal.pone.0060882 Text en © 2013 Nishimura et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Nishimura, Tomohiro
Sato, Shinya
Tawong, Wittaya
Sakanari, Hiroshi
Uehara, Keita
Shah, Md Mahfuzur Rahman
Suda, Shoichiro
Yasumoto, Takeshi
Taira, Yohsuke
Yamaguchi, Haruo
Adachi, Masao
Genetic Diversity and Distribution of the Ciguatera-Causing Dinoflagellate Gambierdiscus spp. (Dinophyceae) in Coastal Areas of Japan
title Genetic Diversity and Distribution of the Ciguatera-Causing Dinoflagellate Gambierdiscus spp. (Dinophyceae) in Coastal Areas of Japan
title_full Genetic Diversity and Distribution of the Ciguatera-Causing Dinoflagellate Gambierdiscus spp. (Dinophyceae) in Coastal Areas of Japan
title_fullStr Genetic Diversity and Distribution of the Ciguatera-Causing Dinoflagellate Gambierdiscus spp. (Dinophyceae) in Coastal Areas of Japan
title_full_unstemmed Genetic Diversity and Distribution of the Ciguatera-Causing Dinoflagellate Gambierdiscus spp. (Dinophyceae) in Coastal Areas of Japan
title_short Genetic Diversity and Distribution of the Ciguatera-Causing Dinoflagellate Gambierdiscus spp. (Dinophyceae) in Coastal Areas of Japan
title_sort genetic diversity and distribution of the ciguatera-causing dinoflagellate gambierdiscus spp. (dinophyceae) in coastal areas of japan
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3623954/
https://www.ncbi.nlm.nih.gov/pubmed/23593339
http://dx.doi.org/10.1371/journal.pone.0060882
work_keys_str_mv AT nishimuratomohiro geneticdiversityanddistributionoftheciguateracausingdinoflagellategambierdiscussppdinophyceaeincoastalareasofjapan
AT satoshinya geneticdiversityanddistributionoftheciguateracausingdinoflagellategambierdiscussppdinophyceaeincoastalareasofjapan
AT tawongwittaya geneticdiversityanddistributionoftheciguateracausingdinoflagellategambierdiscussppdinophyceaeincoastalareasofjapan
AT sakanarihiroshi geneticdiversityanddistributionoftheciguateracausingdinoflagellategambierdiscussppdinophyceaeincoastalareasofjapan
AT ueharakeita geneticdiversityanddistributionoftheciguateracausingdinoflagellategambierdiscussppdinophyceaeincoastalareasofjapan
AT shahmdmahfuzurrahman geneticdiversityanddistributionoftheciguateracausingdinoflagellategambierdiscussppdinophyceaeincoastalareasofjapan
AT sudashoichiro geneticdiversityanddistributionoftheciguateracausingdinoflagellategambierdiscussppdinophyceaeincoastalareasofjapan
AT yasumototakeshi geneticdiversityanddistributionoftheciguateracausingdinoflagellategambierdiscussppdinophyceaeincoastalareasofjapan
AT tairayohsuke geneticdiversityanddistributionoftheciguateracausingdinoflagellategambierdiscussppdinophyceaeincoastalareasofjapan
AT yamaguchiharuo geneticdiversityanddistributionoftheciguateracausingdinoflagellategambierdiscussppdinophyceaeincoastalareasofjapan
AT adachimasao geneticdiversityanddistributionoftheciguateracausingdinoflagellategambierdiscussppdinophyceaeincoastalareasofjapan