Cargando…

Caveolar Fatty Acids and Acylation of Caveolin-1

PURPOSE: Caveolae are cholesterol and sphingolipids rich subcellular domains on plasma membrane. Caveolae contain a variety of signaling proteins which provide platforms for signaling transduction. In addition to enriched with cholesterol and sphingolipids, caveolae also contain a variety of fatty a...

Descripción completa

Detalles Bibliográficos
Autores principales: Cai, Qian, Guo, Ling, Gao, Haiqing, Li, Xiang-An
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3623963/
https://www.ncbi.nlm.nih.gov/pubmed/23593340
http://dx.doi.org/10.1371/journal.pone.0060884
_version_ 1782266001046372352
author Cai, Qian
Guo, Ling
Gao, Haiqing
Li, Xiang-An
author_facet Cai, Qian
Guo, Ling
Gao, Haiqing
Li, Xiang-An
author_sort Cai, Qian
collection PubMed
description PURPOSE: Caveolae are cholesterol and sphingolipids rich subcellular domains on plasma membrane. Caveolae contain a variety of signaling proteins which provide platforms for signaling transduction. In addition to enriched with cholesterol and sphingolipids, caveolae also contain a variety of fatty acids. It has been well-established that acylation of protein plays a pivotal role in subcellular location including targeting to caveolae. However, the fatty acid compositions of caveolae and the type of acylation of caveolar proteins remain largely unknown. In this study, we investigated the fatty acids in caveolae and caveolin-1 bound fatty acids. METHODS: Caveolae were isolated from Chinese hamster ovary (CHO) cells. The caveolar fatty acids were extracted with Folch reagent, methyl esterificated with BF3, and analyzed by gas chromatograph-mass spectrometer (GC/MS). The caveolin-1bound fatty acids were immunoprecipitated by anti-caveolin-1 IgG and analyzed with GC/MS. RESULTS: In contrast to the whole CHO cell lysate which contained a variety of fatty acids, caveolae mainly contained three types of fatty acids, 0.48 µg palmitic acid, 0.61 µg stearic acid and 0.83 µg oleic acid/caveolae preparation/5×10(7) cells. Unexpectedly, GC/MS analysis indicated that caveolin-1 was not acylated by myristic acid; instead, it was acylated by palmitic acid and stearic acid. CONCLUSION: Caveolae contained a special set of fatty acids, highly enriched with saturated fatty acids, and caveolin-1 was acylated by palmitic acid and stearic acid. The unique fatty acid compositions of caveolae and acylation of caveolin-1 may be important for caveolae formation and for maintaining the function of caveolae.
format Online
Article
Text
id pubmed-3623963
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-36239632013-04-16 Caveolar Fatty Acids and Acylation of Caveolin-1 Cai, Qian Guo, Ling Gao, Haiqing Li, Xiang-An PLoS One Research Article PURPOSE: Caveolae are cholesterol and sphingolipids rich subcellular domains on plasma membrane. Caveolae contain a variety of signaling proteins which provide platforms for signaling transduction. In addition to enriched with cholesterol and sphingolipids, caveolae also contain a variety of fatty acids. It has been well-established that acylation of protein plays a pivotal role in subcellular location including targeting to caveolae. However, the fatty acid compositions of caveolae and the type of acylation of caveolar proteins remain largely unknown. In this study, we investigated the fatty acids in caveolae and caveolin-1 bound fatty acids. METHODS: Caveolae were isolated from Chinese hamster ovary (CHO) cells. The caveolar fatty acids were extracted with Folch reagent, methyl esterificated with BF3, and analyzed by gas chromatograph-mass spectrometer (GC/MS). The caveolin-1bound fatty acids were immunoprecipitated by anti-caveolin-1 IgG and analyzed with GC/MS. RESULTS: In contrast to the whole CHO cell lysate which contained a variety of fatty acids, caveolae mainly contained three types of fatty acids, 0.48 µg palmitic acid, 0.61 µg stearic acid and 0.83 µg oleic acid/caveolae preparation/5×10(7) cells. Unexpectedly, GC/MS analysis indicated that caveolin-1 was not acylated by myristic acid; instead, it was acylated by palmitic acid and stearic acid. CONCLUSION: Caveolae contained a special set of fatty acids, highly enriched with saturated fatty acids, and caveolin-1 was acylated by palmitic acid and stearic acid. The unique fatty acid compositions of caveolae and acylation of caveolin-1 may be important for caveolae formation and for maintaining the function of caveolae. Public Library of Science 2013-04-11 /pmc/articles/PMC3623963/ /pubmed/23593340 http://dx.doi.org/10.1371/journal.pone.0060884 Text en © 2013 Cai et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Cai, Qian
Guo, Ling
Gao, Haiqing
Li, Xiang-An
Caveolar Fatty Acids and Acylation of Caveolin-1
title Caveolar Fatty Acids and Acylation of Caveolin-1
title_full Caveolar Fatty Acids and Acylation of Caveolin-1
title_fullStr Caveolar Fatty Acids and Acylation of Caveolin-1
title_full_unstemmed Caveolar Fatty Acids and Acylation of Caveolin-1
title_short Caveolar Fatty Acids and Acylation of Caveolin-1
title_sort caveolar fatty acids and acylation of caveolin-1
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3623963/
https://www.ncbi.nlm.nih.gov/pubmed/23593340
http://dx.doi.org/10.1371/journal.pone.0060884
work_keys_str_mv AT caiqian caveolarfattyacidsandacylationofcaveolin1
AT guoling caveolarfattyacidsandacylationofcaveolin1
AT gaohaiqing caveolarfattyacidsandacylationofcaveolin1
AT lixiangan caveolarfattyacidsandacylationofcaveolin1