Cargando…

Serum-free freezing media support high cell quality and excellent ELISPOT assay performance across a wide variety of different assay protocols

Robust and sensitive ELISPOT protocols are commonly applied concomitant with the development of new immunotherapeutics. Despite the knowledge that individual serum batches differ in their composition and may change properties over time, serum is still commonly used in immunologic assays. Commerciall...

Descripción completa

Detalles Bibliográficos
Autores principales:	Filbert, Helene, Attig, Sebastian, Bidmon, Nicole, Renard, Bernhard Y., Janetzki, Sylvia, Sahin, Ugur, Welters, Marij J. P., Ottensmeier, Christian, van der Burg, Sjoerd H., Gouttefangeas, Cécile, Britten, Cedrik M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer-Verlag 2012
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3624011/ https://www.ncbi.nlm.nih.gov/pubmed/23138872 http://dx.doi.org/10.1007/s00262-012-1359-5

Ejemplares similares

Response definition criteria for ELISPOT assays revisited
por: Moodie, Z., et al.
Publicado: (2010)

Harmonization of the intracellular cytokine staining assay
por: Welters, Marij J. P., et al.
Publicado: (2012)

A framework for T cell assays
por: Britten, Cedrik M., et al.
Publicado: (2015)

Harmonisation of short-term in vitro culture for the expansion of antigen-specific CD8(+) T cells with detection by ELISPOT and HLA-multimer staining
por: Chudley, Lindsey, et al.
Publicado: (2014)

Report on the Fifth Annual Meeting of the Association for Immunotherapy of Cancer (CIMT) April 12–14, 2007 in Würzburg, Germany
por: Welters, Marij J. P., et al.
Publicado: (2007)

Performance of serum-supplemented and serum-free media in IFNγ Elispot Assays for human T cells
por: Janetzki, Sylvia, et al.
Publicado: (2009)

Serum is not required for ex vivo IFN-γ ELISPOT: a collaborative study of different protocols from the European CIMT Immunoguiding Program
por: Mander, A., et al.
Publicado: (2010)

The development of standard samples with a defined number of antigen-specific T cells to harmonize T cell assays: a proof-of-principle study
por: Singh, Satwinder Kaur, et al.
Publicado: (2012)

Managing Multi-center Flow Cytometry Data for Immune Monitoring
por: White, Scott, et al.
Publicado: (2015)

Toward harmonized phenotyping of human myeloid-derived suppressor cells by flow cytometry: results from an interim study
por: Mandruzzato, Susanna, et al.
Publicado: (2016)

Immune monitoring technology primer: the enzyme-linked immunospot (Elispot) and Fluorospot assay
por: Janetzki, Sylvia
Publicado: (2015)

Stepping up ELISpot: Multi-Level Analysis in FluoroSpot Assays
por: Janetzki, Sylvia, et al.
Publicado: (2014)

Hierarchical Modeling for Rare Event Detection and Cell Subset Alignment across Flow Cytometry Samples
por: Cron, Andrew, et al.
Publicado: (2013)

Modified ELISPOT: Modifications of the Elispot Assay for T Cell Monitoring in Cancer Vaccine Trials
por: Malyguine, Anatoli
Publicado: (2005)

Results and harmonization guidelines from two large-scale international Elispot proficiency panels conducted by the Cancer Vaccine Consortium (CVC/SVI)
por: Janetzki, Sylvia, et al.
Publicado: (2007)

Toward the harmonization of immune monitoring in clinical trials: Quo vadis?
por: Britten, C. M., et al.
Publicado: (2007)

ELISPOT Cell Analysis Assay: Searching for Extracellular Footprints
por: Kalyuzhny, Alexander E.
Publicado: (2011)

ELISPOT assays and their diagnostic potential in Lyme disease and Lyme neuroborreliosis
por: Ettischer‐Schmid, N., et al.
Publicado: (2020)

Immunological Monitoring to Rationally Guide AAV Gene Therapy
por: Britten, Cedrik Michael, et al.
Publicado: (2013)

Advancing the field of cancer immunotherapy: MIATA consensus guidelines become available to improve data reporting and interpretation for T-cell immune monitoring
por: Hoos, Axel, et al.
Publicado: (2012)

Equivalence of ELISpot Assays Demonstrated between Major HIV Network Laboratories
por: Gill, Dilbinder K., et al.
Publicado: (2010)

Role of ELISPOT Assays in Risk Assessment Pre- and Post-Kidney Transplantation
por: Zitzner, Jennifer R., et al.
Publicado: (2012)

Dissecting the T Cell Response: Proliferation Assays vs. Cytokine Signatures by ELISPOT
por: Anthony, Donald D., et al.
Publicado: (2012)

The Granzyme B ELISPOT assay: an alternative to the (51)Cr-release assay for monitoring cell-mediated cytotoxicity
por: Shafer-Weaver, Kimberly, et al.
Publicado: (2003)

Detection and functional assessment of regulatory T cells in clinical samples
por: Santegoets, Saskia, et al.
Publicado: (2014)

Harmonization guidelines for HLA-peptide multimer assays derived from results of a large scale international proficiency panel of the Cancer Vaccine Consortium
por: Britten, Cedrik Michael, et al.
Publicado: (2009)

The CIMT-monitoring panel: a two-step approach to harmonize the enumeration of antigen-specific CD8(+) T lymphocytes by structural and functional assays
por: Britten, C. M., et al.
Publicado: (2007)

Evaluating the cytotoxicity of innate immune effector cells using the GrB ELISPOT assay
por: Shafer-Weaver, Kimberly A, et al.
Publicado: (2004)

An Enhanced ELISPOT Assay for Sensitive Detection of Antigen-Specific T Cell Responses to Borrelia burgdorferi
por: Jin, Chenggang, et al.
Publicado: (2013)

ELISPOT Assay for Monitoring Cytotoxic T Lymphocytes (CTL) Activity in Cancer Vaccine Clinical Trials
por: Malyguine, Anatoli M., et al.
Publicado: (2012)

Cryopreservation of MHC Multimers: Recommendations for Quality Assurance in Detection of Antigen Specific T Cells
por: Hadrup, Sine Reker, et al.
Publicado: (2015)

The Tumor Microenvironment and Immunotherapy of Oropharyngeal Squamous Cell Carcinoma
por: Welters, Marij J. P., et al.
Publicado: (2020)

A modified human ELISPOT assay to detect specific responses to primary tumor cell targets
por: Malyguine, Anatoli, et al.
Publicado: (2004)

Resting of Cryopreserved PBMC Does Not Generally Benefit the Performance of Antigen-Specific T Cell ELISPOT Assays
por: Kuerten, Stefanie, et al.
Publicado: (2012)

Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay
por: Grant, Clare FJ, et al.
Publicado: (2012)

ELISPOT Assays in 384-Well Format: Up to 30 Data Points with One Million Cells
por: Hanson, Jodi, et al.
Publicado: (2015)

Establishment of an ELISpot Assay to Detect Cellular Immunity against S. pneumoniae in Vaccinated Kidney Transplant Recipients
por: Gäckler, Anja, et al.
Publicado: (2021)

Sensitivity and specificity of tritiated thymidine incorporation and ELISPOT assays in identifying antigen specific T cell immune responses
por: Goodell, Vivian, et al.
Publicado: (2007)

Towards standardized automated immunomonitoring: an automated ELISpot assay for safe and parallelized functionality analysis of immune cells
por: Neubauer, J. C., et al.
Publicado: (2016)

Cytomegalovirus (CMV) immune monitoring with ELISPOT and QuantiFERON-CMV assay in seropositive kidney transplant recipients
por: Lee, Hyeyoung, et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_1pi7l2ceu7ipnpj8qeij7r8e36