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A Potential Role for CHH DNA Methylation in Cotton Fiber Growth Patterns

DNA methylation controls many aspects of plant growth and development. Here, we report a novel annual growth potential change that may correlate with changes in levels of the major DNA demethylases and methyltransferases in cotton ovules harvested at different times of the year. The abundances of DN...

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Autores principales: Jin, Xiang, Pang, Yu, Jia, Fangxing, Xiao, Guanghui, Li, Qin, Zhu, Yuxian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3625195/
https://www.ncbi.nlm.nih.gov/pubmed/23593241
http://dx.doi.org/10.1371/journal.pone.0060547
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author Jin, Xiang
Pang, Yu
Jia, Fangxing
Xiao, Guanghui
Li, Qin
Zhu, Yuxian
author_facet Jin, Xiang
Pang, Yu
Jia, Fangxing
Xiao, Guanghui
Li, Qin
Zhu, Yuxian
author_sort Jin, Xiang
collection PubMed
description DNA methylation controls many aspects of plant growth and development. Here, we report a novel annual growth potential change that may correlate with changes in levels of the major DNA demethylases and methyltransferases in cotton ovules harvested at different times of the year. The abundances of DNA demethylases, at both the mRNA and protein levels, increased significantly from February to August and decreased during the remainder of the 12-month period, with the opposite pattern observed for DNA methyltransferases. Over the course of one year, substantial changes in methylcytosine content was observed at certain CHH sites (H = A, C, or T) in the promoter regions of the ETHYLENE RESPONSIVE FACTOR 6 (ERF6), SUPPRESSION OF RVS 161 DELTA 4 (SUR4) and 3-KETOACYL-COA SYNTHASE 13 (KCS13), which regulate cotton fiber growth. Three independent techniques were used to confirm the annual fluctuations in DNA methylation. Furthermore, in homozygous RNAi lines specifically targeting REPRESSOR OF SILENCING 1 (ROS1, a conserved DNA demethylase domain), promotion of DNA methylation significantly reduced fiber growth during August.
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spelling pubmed-36251952013-04-16 A Potential Role for CHH DNA Methylation in Cotton Fiber Growth Patterns Jin, Xiang Pang, Yu Jia, Fangxing Xiao, Guanghui Li, Qin Zhu, Yuxian PLoS One Research Article DNA methylation controls many aspects of plant growth and development. Here, we report a novel annual growth potential change that may correlate with changes in levels of the major DNA demethylases and methyltransferases in cotton ovules harvested at different times of the year. The abundances of DNA demethylases, at both the mRNA and protein levels, increased significantly from February to August and decreased during the remainder of the 12-month period, with the opposite pattern observed for DNA methyltransferases. Over the course of one year, substantial changes in methylcytosine content was observed at certain CHH sites (H = A, C, or T) in the promoter regions of the ETHYLENE RESPONSIVE FACTOR 6 (ERF6), SUPPRESSION OF RVS 161 DELTA 4 (SUR4) and 3-KETOACYL-COA SYNTHASE 13 (KCS13), which regulate cotton fiber growth. Three independent techniques were used to confirm the annual fluctuations in DNA methylation. Furthermore, in homozygous RNAi lines specifically targeting REPRESSOR OF SILENCING 1 (ROS1, a conserved DNA demethylase domain), promotion of DNA methylation significantly reduced fiber growth during August. Public Library of Science 2013-04-12 /pmc/articles/PMC3625195/ /pubmed/23593241 http://dx.doi.org/10.1371/journal.pone.0060547 Text en © 2013 Jin et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Jin, Xiang
Pang, Yu
Jia, Fangxing
Xiao, Guanghui
Li, Qin
Zhu, Yuxian
A Potential Role for CHH DNA Methylation in Cotton Fiber Growth Patterns
title A Potential Role for CHH DNA Methylation in Cotton Fiber Growth Patterns
title_full A Potential Role for CHH DNA Methylation in Cotton Fiber Growth Patterns
title_fullStr A Potential Role for CHH DNA Methylation in Cotton Fiber Growth Patterns
title_full_unstemmed A Potential Role for CHH DNA Methylation in Cotton Fiber Growth Patterns
title_short A Potential Role for CHH DNA Methylation in Cotton Fiber Growth Patterns
title_sort potential role for chh dna methylation in cotton fiber growth patterns
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3625195/
https://www.ncbi.nlm.nih.gov/pubmed/23593241
http://dx.doi.org/10.1371/journal.pone.0060547
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