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Structure-Function-Immunogenicity Studies of PfEMP1 Domain DBL2β(PF11_0521), a Malaria Parasite Ligand for ICAM-1
Plasmodium falciparum virulence has been ascribed to its ability to sequester in deep vascular beds, mediated by the variant surface antigen family PfEMP1 binding endothelial receptors like ICAM-1. We previously observed that naturally-acquired antibodies that block a PfEMP1 domain, DBL2β of PF11_05...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2013
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3625211/ https://www.ncbi.nlm.nih.gov/pubmed/23593462 http://dx.doi.org/10.1371/journal.pone.0061323 |
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author | Gullingsrud, Justin Saveria, Tracy Amos, Emily Duffy, Patrick E. Oleinikov, Andrew V. |
author_facet | Gullingsrud, Justin Saveria, Tracy Amos, Emily Duffy, Patrick E. Oleinikov, Andrew V. |
author_sort | Gullingsrud, Justin |
collection | PubMed |
description | Plasmodium falciparum virulence has been ascribed to its ability to sequester in deep vascular beds, mediated by the variant surface antigen family PfEMP1 binding endothelial receptors like ICAM-1. We previously observed that naturally-acquired antibodies that block a PfEMP1 domain, DBL2β of PF11_0521 allele, from binding to the human ICAM1 receptor, reduce the risk of malaria hospitalization in children. Here, we find that DBL2β(PF11_0521) binds ICAM-1 in the low nM range and relate the structure of this domain with its function and immunogenicity. We demonstrate that the interaction with ICAM-1 is not impaired by point mutations in the N-terminal subdomain or in the flexible Loop 4 of DBL2β(PF11_0521), although both substructures were previously implicated in binding ICAM-1. These data will help to refine the existing model of DBLβ::ICAM-1 interactions. Antibodies raised against full-length DBL2β(PF11_0521), but not truncated forms lacking the N terminal fragment, block its interaction with ICAM-1. Our data suggest that full length domain is optimal for displaying functional epitopes and has a broad surface of interaction with ICAM-1 that is not disrupted by individual amino acid substitutions at putative key residues. This information might be important for the future design of anti-malarial vaccines based on PfEMP1 antigens. |
format | Online Article Text |
id | pubmed-3625211 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36252112013-04-16 Structure-Function-Immunogenicity Studies of PfEMP1 Domain DBL2β(PF11_0521), a Malaria Parasite Ligand for ICAM-1 Gullingsrud, Justin Saveria, Tracy Amos, Emily Duffy, Patrick E. Oleinikov, Andrew V. PLoS One Research Article Plasmodium falciparum virulence has been ascribed to its ability to sequester in deep vascular beds, mediated by the variant surface antigen family PfEMP1 binding endothelial receptors like ICAM-1. We previously observed that naturally-acquired antibodies that block a PfEMP1 domain, DBL2β of PF11_0521 allele, from binding to the human ICAM1 receptor, reduce the risk of malaria hospitalization in children. Here, we find that DBL2β(PF11_0521) binds ICAM-1 in the low nM range and relate the structure of this domain with its function and immunogenicity. We demonstrate that the interaction with ICAM-1 is not impaired by point mutations in the N-terminal subdomain or in the flexible Loop 4 of DBL2β(PF11_0521), although both substructures were previously implicated in binding ICAM-1. These data will help to refine the existing model of DBLβ::ICAM-1 interactions. Antibodies raised against full-length DBL2β(PF11_0521), but not truncated forms lacking the N terminal fragment, block its interaction with ICAM-1. Our data suggest that full length domain is optimal for displaying functional epitopes and has a broad surface of interaction with ICAM-1 that is not disrupted by individual amino acid substitutions at putative key residues. This information might be important for the future design of anti-malarial vaccines based on PfEMP1 antigens. Public Library of Science 2013-04-12 /pmc/articles/PMC3625211/ /pubmed/23593462 http://dx.doi.org/10.1371/journal.pone.0061323 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. |
spellingShingle | Research Article Gullingsrud, Justin Saveria, Tracy Amos, Emily Duffy, Patrick E. Oleinikov, Andrew V. Structure-Function-Immunogenicity Studies of PfEMP1 Domain DBL2β(PF11_0521), a Malaria Parasite Ligand for ICAM-1 |
title | Structure-Function-Immunogenicity Studies of PfEMP1 Domain DBL2β(PF11_0521), a Malaria Parasite Ligand for ICAM-1 |
title_full | Structure-Function-Immunogenicity Studies of PfEMP1 Domain DBL2β(PF11_0521), a Malaria Parasite Ligand for ICAM-1 |
title_fullStr | Structure-Function-Immunogenicity Studies of PfEMP1 Domain DBL2β(PF11_0521), a Malaria Parasite Ligand for ICAM-1 |
title_full_unstemmed | Structure-Function-Immunogenicity Studies of PfEMP1 Domain DBL2β(PF11_0521), a Malaria Parasite Ligand for ICAM-1 |
title_short | Structure-Function-Immunogenicity Studies of PfEMP1 Domain DBL2β(PF11_0521), a Malaria Parasite Ligand for ICAM-1 |
title_sort | structure-function-immunogenicity studies of pfemp1 domain dbl2β(pf11_0521), a malaria parasite ligand for icam-1 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3625211/ https://www.ncbi.nlm.nih.gov/pubmed/23593462 http://dx.doi.org/10.1371/journal.pone.0061323 |
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