Clostridium botulinum Strain Af84 Contains Three Neurotoxin Gene Clusters: Bont/A2, bont/F4 and bont/F5

Sanger and shotgun sequencing of Clostridium botulinum strain Af84 type Af and its botulinum neurotoxin gene (bont) clusters identified the presence of three bont gene clusters rather than the expected two. The three toxin gene clusters consisted of bont subtypes A2, F4 and F5. The bont/A2 and bont/...

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Autores principales: Dover, Nir, Barash, Jason R., Hill, Karen K., Davenport, Karen W., Teshima, Hazuki, Xie, Gary, Arnon, Stephen S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3625220/
https://www.ncbi.nlm.nih.gov/pubmed/23637798
http://dx.doi.org/10.1371/journal.pone.0061205
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author Dover, Nir
Barash, Jason R.
Hill, Karen K.
Davenport, Karen W.
Teshima, Hazuki
Xie, Gary
Arnon, Stephen S.
author_facet Dover, Nir
Barash, Jason R.
Hill, Karen K.
Davenport, Karen W.
Teshima, Hazuki
Xie, Gary
Arnon, Stephen S.
author_sort Dover, Nir
collection PubMed
description Sanger and shotgun sequencing of Clostridium botulinum strain Af84 type Af and its botulinum neurotoxin gene (bont) clusters identified the presence of three bont gene clusters rather than the expected two. The three toxin gene clusters consisted of bont subtypes A2, F4 and F5. The bont/A2 and bont/F4 gene clusters were located within the chromosome (the latter in a novel location), while the bont/F5 toxin gene cluster was located within a large 246 kb plasmid. These findings are the first identification of a C. botulinum strain that contains three botulinum neurotoxin gene clusters.
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spelling pubmed-36252202013-05-01 Clostridium botulinum Strain Af84 Contains Three Neurotoxin Gene Clusters: Bont/A2, bont/F4 and bont/F5 Dover, Nir Barash, Jason R. Hill, Karen K. Davenport, Karen W. Teshima, Hazuki Xie, Gary Arnon, Stephen S. PLoS One Research Article Sanger and shotgun sequencing of Clostridium botulinum strain Af84 type Af and its botulinum neurotoxin gene (bont) clusters identified the presence of three bont gene clusters rather than the expected two. The three toxin gene clusters consisted of bont subtypes A2, F4 and F5. The bont/A2 and bont/F4 gene clusters were located within the chromosome (the latter in a novel location), while the bont/F5 toxin gene cluster was located within a large 246 kb plasmid. These findings are the first identification of a C. botulinum strain that contains three botulinum neurotoxin gene clusters. Public Library of Science 2013-04-12 /pmc/articles/PMC3625220/ /pubmed/23637798 http://dx.doi.org/10.1371/journal.pone.0061205 Text en © 2013 Dover et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Dover, Nir
Barash, Jason R.
Hill, Karen K.
Davenport, Karen W.
Teshima, Hazuki
Xie, Gary
Arnon, Stephen S.
Clostridium botulinum Strain Af84 Contains Three Neurotoxin Gene Clusters: Bont/A2, bont/F4 and bont/F5
title Clostridium botulinum Strain Af84 Contains Three Neurotoxin Gene Clusters: Bont/A2, bont/F4 and bont/F5
title_full Clostridium botulinum Strain Af84 Contains Three Neurotoxin Gene Clusters: Bont/A2, bont/F4 and bont/F5
title_fullStr Clostridium botulinum Strain Af84 Contains Three Neurotoxin Gene Clusters: Bont/A2, bont/F4 and bont/F5
title_full_unstemmed Clostridium botulinum Strain Af84 Contains Three Neurotoxin Gene Clusters: Bont/A2, bont/F4 and bont/F5
title_short Clostridium botulinum Strain Af84 Contains Three Neurotoxin Gene Clusters: Bont/A2, bont/F4 and bont/F5
title_sort clostridium botulinum strain af84 contains three neurotoxin gene clusters: bont/a2, bont/f4 and bont/f5
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3625220/
https://www.ncbi.nlm.nih.gov/pubmed/23637798
http://dx.doi.org/10.1371/journal.pone.0061205
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