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Chronological changes in the collagen-type composition at tendon–bone interface in rabbits
OBJECTIVES: The purpose of this study was to evaluate chronological changes in the collagen-type composition at tendon–bone interface during tendon–bone healing and to clarify the continuity between Sharpey-like fibres and inner fibres of the tendon. METHODS: Male white rabbits were used to create a...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
British Editorial Society of Bone and Joint Surgery
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3626213/ https://www.ncbi.nlm.nih.gov/pubmed/23610694 http://dx.doi.org/10.1302/2046-3758.19.2000109 |
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author | Tabuchi, K. Soejima, T. Kanazawa, T. Noguchi, K. Nagata, K. |
author_facet | Tabuchi, K. Soejima, T. Kanazawa, T. Noguchi, K. Nagata, K. |
author_sort | Tabuchi, K. |
collection | PubMed |
description | OBJECTIVES: The purpose of this study was to evaluate chronological changes in the collagen-type composition at tendon–bone interface during tendon–bone healing and to clarify the continuity between Sharpey-like fibres and inner fibres of the tendon. METHODS: Male white rabbits were used to create an extra-articular bone–tendon graft model by grafting the extensor digitorum longus into a bone tunnel. Three rabbits were killed at two, four, eight, 12 and 26 weeks post-operatively. Elastica van Gieson staining was used to colour 5 µm coronal sections, which were examined under optical and polarised light microscopy. Immunostaining for type I, II and III collagen was also performed. RESULTS: Sharpey-like fibres comprised of type III collagen in the early phase were gradually replaced by type I collagen from 12 weeks onwards, until continuity between the Sharpey-like fibres and inner fibres of the tendon was achieved by 26 weeks. CONCLUSIONS: Even in rabbits, which heal faster than humans, an observation period of at least 12 to 26 weeks is required, because the collagen-type composition of the Sharpey-like fibre bone–tendon connection may have insufficient pullout strength during this period. These results suggest that caution is necessary when permitting post-operative activity in humans who have undergone intra-bone tunnel grafts. |
format | Online Article Text |
id | pubmed-3626213 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | British Editorial Society of Bone and Joint Surgery |
record_format | MEDLINE/PubMed |
spelling | pubmed-36262132013-04-22 Chronological changes in the collagen-type composition at tendon–bone interface in rabbits Tabuchi, K. Soejima, T. Kanazawa, T. Noguchi, K. Nagata, K. Bone Joint Res Research OBJECTIVES: The purpose of this study was to evaluate chronological changes in the collagen-type composition at tendon–bone interface during tendon–bone healing and to clarify the continuity between Sharpey-like fibres and inner fibres of the tendon. METHODS: Male white rabbits were used to create an extra-articular bone–tendon graft model by grafting the extensor digitorum longus into a bone tunnel. Three rabbits were killed at two, four, eight, 12 and 26 weeks post-operatively. Elastica van Gieson staining was used to colour 5 µm coronal sections, which were examined under optical and polarised light microscopy. Immunostaining for type I, II and III collagen was also performed. RESULTS: Sharpey-like fibres comprised of type III collagen in the early phase were gradually replaced by type I collagen from 12 weeks onwards, until continuity between the Sharpey-like fibres and inner fibres of the tendon was achieved by 26 weeks. CONCLUSIONS: Even in rabbits, which heal faster than humans, an observation period of at least 12 to 26 weeks is required, because the collagen-type composition of the Sharpey-like fibre bone–tendon connection may have insufficient pullout strength during this period. These results suggest that caution is necessary when permitting post-operative activity in humans who have undergone intra-bone tunnel grafts. British Editorial Society of Bone and Joint Surgery 2012-09-01 /pmc/articles/PMC3626213/ /pubmed/23610694 http://dx.doi.org/10.1302/2046-3758.19.2000109 Text en ©2012 British Editorial Society of Bone and Joint Surgery This is an open-access article distributed under the terms of the Creative Commons Attributions licence, which permits unrestricted use, distribution, and reproduction in any medium, but not for commercial gain, provided the original author and source are credited. |
spellingShingle | Research Tabuchi, K. Soejima, T. Kanazawa, T. Noguchi, K. Nagata, K. Chronological changes in the collagen-type composition at tendon–bone interface in rabbits |
title | Chronological changes in the collagen-type composition
at tendon–bone interface in rabbits |
title_full | Chronological changes in the collagen-type composition
at tendon–bone interface in rabbits |
title_fullStr | Chronological changes in the collagen-type composition
at tendon–bone interface in rabbits |
title_full_unstemmed | Chronological changes in the collagen-type composition
at tendon–bone interface in rabbits |
title_short | Chronological changes in the collagen-type composition
at tendon–bone interface in rabbits |
title_sort | chronological changes in the collagen-type composition
at tendon–bone interface in rabbits |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3626213/ https://www.ncbi.nlm.nih.gov/pubmed/23610694 http://dx.doi.org/10.1302/2046-3758.19.2000109 |
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