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Development of inexpensive and globally available larval diet for rearing Anopheles stephensi (Diptera: Culicidae) mosquitoes

BACKGROUND: Success of sterile insect technique (SIT) is dependent upon the mass rearing and release of quality insects, the production of which is directly related to the suitability of the diet ingredients used. Commercial diets used for small-scale culture of mosquitoes are expensive and thus not...

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Autores principales: Khan, Inamullah, Farid, Abid, Zeb, Alam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3626612/
https://www.ncbi.nlm.nih.gov/pubmed/23570246
http://dx.doi.org/10.1186/1756-3305-6-90
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author Khan, Inamullah
Farid, Abid
Zeb, Alam
author_facet Khan, Inamullah
Farid, Abid
Zeb, Alam
author_sort Khan, Inamullah
collection PubMed
description BACKGROUND: Success of sterile insect technique (SIT) is dependent upon the mass rearing and release of quality insects, the production of which is directly related to the suitability of the diet ingredients used. Commercial diets used for small-scale culture of mosquitoes are expensive and thus not feasible for mass production. METHODS: A series of low cost globally available diet ingredients including, wheat, rice, corn, chickpeas, and beans along with liver, were provided to 4 h larvae (L1) of Anopheles stephensi (Liston) to see their effect on fitness parameters including larval duration, percent emergence, survival, adult wing size and female fecundity. Different quantities of the candidate diet ingredients were then mixed together to work out a combination diet with a balanced nutritive value that can be used for efficient rearing of the mosquito larvae at relatively lower costs. RESULTS: Fastest larval and pupal development and highest survival rates were recorded using a combination diet of bean, corn, wheat, chickpea, rice, and bovine liver at 5 mg/day. The diet is easy to prepare, and much cheaper than the diets reported earlier. The estimated cost of the reported diet is 14.7 US$/ 1.3 kg for rearing one million larvae. CONCLUSIONS: A combination diet with ingredients from cereals and legumes mixed with liver is a low cost balanced larval diet with the potential for use in both small scale laboratory rearing and mass production of Anopheles in SIT control programs.
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spelling pubmed-36266122013-04-16 Development of inexpensive and globally available larval diet for rearing Anopheles stephensi (Diptera: Culicidae) mosquitoes Khan, Inamullah Farid, Abid Zeb, Alam Parasit Vectors Research BACKGROUND: Success of sterile insect technique (SIT) is dependent upon the mass rearing and release of quality insects, the production of which is directly related to the suitability of the diet ingredients used. Commercial diets used for small-scale culture of mosquitoes are expensive and thus not feasible for mass production. METHODS: A series of low cost globally available diet ingredients including, wheat, rice, corn, chickpeas, and beans along with liver, were provided to 4 h larvae (L1) of Anopheles stephensi (Liston) to see their effect on fitness parameters including larval duration, percent emergence, survival, adult wing size and female fecundity. Different quantities of the candidate diet ingredients were then mixed together to work out a combination diet with a balanced nutritive value that can be used for efficient rearing of the mosquito larvae at relatively lower costs. RESULTS: Fastest larval and pupal development and highest survival rates were recorded using a combination diet of bean, corn, wheat, chickpea, rice, and bovine liver at 5 mg/day. The diet is easy to prepare, and much cheaper than the diets reported earlier. The estimated cost of the reported diet is 14.7 US$/ 1.3 kg for rearing one million larvae. CONCLUSIONS: A combination diet with ingredients from cereals and legumes mixed with liver is a low cost balanced larval diet with the potential for use in both small scale laboratory rearing and mass production of Anopheles in SIT control programs. BioMed Central 2013-04-09 /pmc/articles/PMC3626612/ /pubmed/23570246 http://dx.doi.org/10.1186/1756-3305-6-90 Text en Copyright © 2013 Khan et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Khan, Inamullah
Farid, Abid
Zeb, Alam
Development of inexpensive and globally available larval diet for rearing Anopheles stephensi (Diptera: Culicidae) mosquitoes
title Development of inexpensive and globally available larval diet for rearing Anopheles stephensi (Diptera: Culicidae) mosquitoes
title_full Development of inexpensive and globally available larval diet for rearing Anopheles stephensi (Diptera: Culicidae) mosquitoes
title_fullStr Development of inexpensive and globally available larval diet for rearing Anopheles stephensi (Diptera: Culicidae) mosquitoes
title_full_unstemmed Development of inexpensive and globally available larval diet for rearing Anopheles stephensi (Diptera: Culicidae) mosquitoes
title_short Development of inexpensive and globally available larval diet for rearing Anopheles stephensi (Diptera: Culicidae) mosquitoes
title_sort development of inexpensive and globally available larval diet for rearing anopheles stephensi (diptera: culicidae) mosquitoes
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3626612/
https://www.ncbi.nlm.nih.gov/pubmed/23570246
http://dx.doi.org/10.1186/1756-3305-6-90
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