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The Novel Pro-Osteogenic Activity of NUCB2(1–83)
NUCB2(1–83) has been recently reported as an anorexigenic and anti-hyperglycemic peptide. Here we report that NUCB2(1–83) promotes osteogenesis. It was found after two months of once-a-day intravenous injection of NUCB2(1–83), bone mineral density of femora and lumbar vertebrae were increased in ova...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3626684/ https://www.ncbi.nlm.nih.gov/pubmed/23613885 http://dx.doi.org/10.1371/journal.pone.0061619 |
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author | Li, Ruishu Wu, Qiong Zhao, Yue Jin, Wenbo Yuan, Xinfang Wu, Xiaopeng Tang, Yanchun Zhang, Jing Tan, Xiangyang Bi, Feng Liu, Jian-Ning |
author_facet | Li, Ruishu Wu, Qiong Zhao, Yue Jin, Wenbo Yuan, Xinfang Wu, Xiaopeng Tang, Yanchun Zhang, Jing Tan, Xiangyang Bi, Feng Liu, Jian-Ning |
author_sort | Li, Ruishu |
collection | PubMed |
description | NUCB2(1–83) has been recently reported as an anorexigenic and anti-hyperglycemic peptide. Here we report that NUCB2(1–83) promotes osteogenesis. It was found after two months of once-a-day intravenous injection of NUCB2(1–83), bone mineral density of femora and lumbar vertebrae were increased in ovariectomized rats. NUCB2(1–83) also increased the alkaline phosphatase activity and promoted mineralization in mouse MC3T3-E1 preosteoblastic cell line. When either both Arg(60) and Arg(63) or Ser(72) were mutated to Ala, the pro-osteogenic activity was completely lost, indicating that these residues are structurally important for its biological function. Furthermore, it encumbered osteoclastic differentiation of RAW 264.7 macrophage. It also excluded any possibility of the effect caused by contaminants or experimental faults, and demonstrated that the pro-osteogenic activity observed was a specific effect of NUCB2(1–83) itself. These findings warranted that further studies on NUCB2(1–83) would be valuable for the treatment of bone metabolic diseases especially for osteoporosis. |
format | Online Article Text |
id | pubmed-3626684 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36266842013-04-23 The Novel Pro-Osteogenic Activity of NUCB2(1–83) Li, Ruishu Wu, Qiong Zhao, Yue Jin, Wenbo Yuan, Xinfang Wu, Xiaopeng Tang, Yanchun Zhang, Jing Tan, Xiangyang Bi, Feng Liu, Jian-Ning PLoS One Research Article NUCB2(1–83) has been recently reported as an anorexigenic and anti-hyperglycemic peptide. Here we report that NUCB2(1–83) promotes osteogenesis. It was found after two months of once-a-day intravenous injection of NUCB2(1–83), bone mineral density of femora and lumbar vertebrae were increased in ovariectomized rats. NUCB2(1–83) also increased the alkaline phosphatase activity and promoted mineralization in mouse MC3T3-E1 preosteoblastic cell line. When either both Arg(60) and Arg(63) or Ser(72) were mutated to Ala, the pro-osteogenic activity was completely lost, indicating that these residues are structurally important for its biological function. Furthermore, it encumbered osteoclastic differentiation of RAW 264.7 macrophage. It also excluded any possibility of the effect caused by contaminants or experimental faults, and demonstrated that the pro-osteogenic activity observed was a specific effect of NUCB2(1–83) itself. These findings warranted that further studies on NUCB2(1–83) would be valuable for the treatment of bone metabolic diseases especially for osteoporosis. Public Library of Science 2013-04-15 /pmc/articles/PMC3626684/ /pubmed/23613885 http://dx.doi.org/10.1371/journal.pone.0061619 Text en © 2013 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Li, Ruishu Wu, Qiong Zhao, Yue Jin, Wenbo Yuan, Xinfang Wu, Xiaopeng Tang, Yanchun Zhang, Jing Tan, Xiangyang Bi, Feng Liu, Jian-Ning The Novel Pro-Osteogenic Activity of NUCB2(1–83) |
title | The Novel Pro-Osteogenic Activity of NUCB2(1–83)
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title_full | The Novel Pro-Osteogenic Activity of NUCB2(1–83)
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title_fullStr | The Novel Pro-Osteogenic Activity of NUCB2(1–83)
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title_full_unstemmed | The Novel Pro-Osteogenic Activity of NUCB2(1–83)
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title_short | The Novel Pro-Osteogenic Activity of NUCB2(1–83)
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title_sort | novel pro-osteogenic activity of nucb2(1–83) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3626684/ https://www.ncbi.nlm.nih.gov/pubmed/23613885 http://dx.doi.org/10.1371/journal.pone.0061619 |
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