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Global Transcriptome Sequencing Identifies Chlamydospore Specific Markers in Candida albicans and Candida dubliniensis

Candida albicans and Candida dubliniensis are pathogenic fungi that are highly related but differ in virulence and in some phenotypic traits. During in vitro growth on certain nutrient-poor media, C. albicans and C. dubliniensis are the only yeast species which are able to produce chlamydospores, la...

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Autores principales: Palige, Katja, Linde, Jörg, Martin, Ronny, Böttcher, Bettina, Citiulo, Francesco, Sullivan, Derek J., Weber, Johann, Staib, Claudia, Rupp, Steffen, Hube, Bernhard, Morschhäuser, Joachim, Staib, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3626690/
https://www.ncbi.nlm.nih.gov/pubmed/23613980
http://dx.doi.org/10.1371/journal.pone.0061940
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author Palige, Katja
Linde, Jörg
Martin, Ronny
Böttcher, Bettina
Citiulo, Francesco
Sullivan, Derek J.
Weber, Johann
Staib, Claudia
Rupp, Steffen
Hube, Bernhard
Morschhäuser, Joachim
Staib, Peter
author_facet Palige, Katja
Linde, Jörg
Martin, Ronny
Böttcher, Bettina
Citiulo, Francesco
Sullivan, Derek J.
Weber, Johann
Staib, Claudia
Rupp, Steffen
Hube, Bernhard
Morschhäuser, Joachim
Staib, Peter
author_sort Palige, Katja
collection PubMed
description Candida albicans and Candida dubliniensis are pathogenic fungi that are highly related but differ in virulence and in some phenotypic traits. During in vitro growth on certain nutrient-poor media, C. albicans and C. dubliniensis are the only yeast species which are able to produce chlamydospores, large thick-walled cells of unknown function. Interestingly, only C. dubliniensis forms pseudohyphae with abundant chlamydospores when grown on Staib medium, while C. albicans grows exclusively as a budding yeast. In order to further our understanding of chlamydospore development and assembly, we compared the global transcriptional profile of both species during growth in liquid Staib medium by RNA sequencing. We also included a C. albicans mutant in our study which lacks the morphogenetic transcriptional repressor Nrg1. This strain, which is characterized by its constitutive pseudohyphal growth, specifically produces masses of chlamydospores in Staib medium, similar to C. dubliniensis. This comparative approach identified a set of putatively chlamydospore-related genes. Two of the homologous C. albicans and C. dubliniensis genes (CSP1 and CSP2) which were most strongly upregulated during chlamydospore development were analysed in more detail. By use of the green fluorescent protein as a reporter, the encoded putative cell wall related proteins were found to exclusively localize to C. albicans and C. dubliniensis chlamydospores. Our findings uncover the first chlamydospore specific markers in Candida species and provide novel insights in the complex morphogenetic development of these important fungal pathogens.
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spelling pubmed-36266902013-04-23 Global Transcriptome Sequencing Identifies Chlamydospore Specific Markers in Candida albicans and Candida dubliniensis Palige, Katja Linde, Jörg Martin, Ronny Böttcher, Bettina Citiulo, Francesco Sullivan, Derek J. Weber, Johann Staib, Claudia Rupp, Steffen Hube, Bernhard Morschhäuser, Joachim Staib, Peter PLoS One Research Article Candida albicans and Candida dubliniensis are pathogenic fungi that are highly related but differ in virulence and in some phenotypic traits. During in vitro growth on certain nutrient-poor media, C. albicans and C. dubliniensis are the only yeast species which are able to produce chlamydospores, large thick-walled cells of unknown function. Interestingly, only C. dubliniensis forms pseudohyphae with abundant chlamydospores when grown on Staib medium, while C. albicans grows exclusively as a budding yeast. In order to further our understanding of chlamydospore development and assembly, we compared the global transcriptional profile of both species during growth in liquid Staib medium by RNA sequencing. We also included a C. albicans mutant in our study which lacks the morphogenetic transcriptional repressor Nrg1. This strain, which is characterized by its constitutive pseudohyphal growth, specifically produces masses of chlamydospores in Staib medium, similar to C. dubliniensis. This comparative approach identified a set of putatively chlamydospore-related genes. Two of the homologous C. albicans and C. dubliniensis genes (CSP1 and CSP2) which were most strongly upregulated during chlamydospore development were analysed in more detail. By use of the green fluorescent protein as a reporter, the encoded putative cell wall related proteins were found to exclusively localize to C. albicans and C. dubliniensis chlamydospores. Our findings uncover the first chlamydospore specific markers in Candida species and provide novel insights in the complex morphogenetic development of these important fungal pathogens. Public Library of Science 2013-04-15 /pmc/articles/PMC3626690/ /pubmed/23613980 http://dx.doi.org/10.1371/journal.pone.0061940 Text en © 2013 Palige et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Palige, Katja
Linde, Jörg
Martin, Ronny
Böttcher, Bettina
Citiulo, Francesco
Sullivan, Derek J.
Weber, Johann
Staib, Claudia
Rupp, Steffen
Hube, Bernhard
Morschhäuser, Joachim
Staib, Peter
Global Transcriptome Sequencing Identifies Chlamydospore Specific Markers in Candida albicans and Candida dubliniensis
title Global Transcriptome Sequencing Identifies Chlamydospore Specific Markers in Candida albicans and Candida dubliniensis
title_full Global Transcriptome Sequencing Identifies Chlamydospore Specific Markers in Candida albicans and Candida dubliniensis
title_fullStr Global Transcriptome Sequencing Identifies Chlamydospore Specific Markers in Candida albicans and Candida dubliniensis
title_full_unstemmed Global Transcriptome Sequencing Identifies Chlamydospore Specific Markers in Candida albicans and Candida dubliniensis
title_short Global Transcriptome Sequencing Identifies Chlamydospore Specific Markers in Candida albicans and Candida dubliniensis
title_sort global transcriptome sequencing identifies chlamydospore specific markers in candida albicans and candida dubliniensis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3626690/
https://www.ncbi.nlm.nih.gov/pubmed/23613980
http://dx.doi.org/10.1371/journal.pone.0061940
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