Demonstration of protein-fragment complementation assay using purified firefly luciferase fragments

BACKGROUND: Human interactome is predicted to contain 150,000 to 300,000 protein-protein interactions, (PPIs). Protein-fragment complementation assay (PCA) is one of the most widely used methods to detect PPI, as well as Förster resonance energy transfer (FRET). To date, successful applications of f...

Descripción completa

Detalles Bibliográficos
Autores principales: Ohmuro-Matsuyama, Yuki, Chung, Chan-I, Ueda, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3626928/
https://www.ncbi.nlm.nih.gov/pubmed/23536995
http://dx.doi.org/10.1186/1472-6750-13-31

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3626928/
https://www.ncbi.nlm.nih.gov/pubmed/23536995
http://dx.doi.org/10.1186/1472-6750-13-31

Ejemplares similares