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Demonstration of protein-fragment complementation assay using purified firefly luciferase fragments
BACKGROUND: Human interactome is predicted to contain 150,000 to 300,000 protein-protein interactions, (PPIs). Protein-fragment complementation assay (PCA) is one of the most widely used methods to detect PPI, as well as Förster resonance energy transfer (FRET). To date, successful applications of f...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2013
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3626928/ https://www.ncbi.nlm.nih.gov/pubmed/23536995 http://dx.doi.org/10.1186/1472-6750-13-31 |