Development and validation of qualitative SYBR®Green Real-Time PCR for detection and discrimination of Listeria spp. and Listeria monocytogenes

A combination of four qualitative SYBR®Green qPCR screening assays targeting two levels of discrimination: Listeria genus (except Listeria grayi) and Listeria monocytogenes, is presented. These assays have been developed to be run simultaneously using the same polymerase chain reaction (PCR) program...

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Detalles Bibliográficos
Autores principales: Barbau-Piednoir, Elodie, Botteldoorn, Nadine, Yde, Marc, Mahillon, Jacques, Roosens, Nancy H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2012
Materias:
Applied Genetics and Molecular Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3627020/
https://www.ncbi.nlm.nih.gov/pubmed/23086339
http://dx.doi.org/10.1007/s00253-012-4477-2

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