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Liesegang-like patterns of Toll crystals grown in gel
Generating high-quality crystals remains a bottleneck in biological and materials sciences. Here a counter-diffusion method was used to improve the X-ray diffraction quality of the N-terminal domain of Drosophila melanogaster Toll receptor crystals. It was observed that crystallization occurred with...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3627410/ https://www.ncbi.nlm.nih.gov/pubmed/23596340 http://dx.doi.org/10.1107/S0021889812051606 |
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author | Gangloff, Monique Moreno, Abel Gay, Nicholas J. |
author_facet | Gangloff, Monique Moreno, Abel Gay, Nicholas J. |
author_sort | Gangloff, Monique |
collection | PubMed |
description | Generating high-quality crystals remains a bottleneck in biological and materials sciences. Here a counter-diffusion method was used to improve the X-ray diffraction quality of the N-terminal domain of Drosophila melanogaster Toll receptor crystals. It was observed that crystallization occurred with a peculiar pattern along the capillary resembling Liesegang bands; this phenomenon is described at both macroscopic and atomic levels. It was found that bands appeared for native protein as well as for co-crystals of magic triangle (I3C)-bound protein even though they crystallize in different space groups. Crystallization occurred with a linear recurrence independent of the precipitant concentration and a protein-specific spacing coefficient. Bandwidth varied along the capillary, oscillating between large precipitation areas and single crystals. The reported data suggest that repetitive patterns can be generated with biological macromolecules in the presence of sodium malonate as a crystallization agent. A comparison with typical Liesegang patterns and the possible mechanism underlying this phenomenon are discussed. |
format | Online Article Text |
id | pubmed-3627410 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-36274102013-04-17 Liesegang-like patterns of Toll crystals grown in gel Gangloff, Monique Moreno, Abel Gay, Nicholas J. J Appl Crystallogr Research Papers Generating high-quality crystals remains a bottleneck in biological and materials sciences. Here a counter-diffusion method was used to improve the X-ray diffraction quality of the N-terminal domain of Drosophila melanogaster Toll receptor crystals. It was observed that crystallization occurred with a peculiar pattern along the capillary resembling Liesegang bands; this phenomenon is described at both macroscopic and atomic levels. It was found that bands appeared for native protein as well as for co-crystals of magic triangle (I3C)-bound protein even though they crystallize in different space groups. Crystallization occurred with a linear recurrence independent of the precipitant concentration and a protein-specific spacing coefficient. Bandwidth varied along the capillary, oscillating between large precipitation areas and single crystals. The reported data suggest that repetitive patterns can be generated with biological macromolecules in the presence of sodium malonate as a crystallization agent. A comparison with typical Liesegang patterns and the possible mechanism underlying this phenomenon are discussed. International Union of Crystallography 2013-02-14 /pmc/articles/PMC3627410/ /pubmed/23596340 http://dx.doi.org/10.1107/S0021889812051606 Text en © Monique Gangloff et al. 2013 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Research Papers Gangloff, Monique Moreno, Abel Gay, Nicholas J. Liesegang-like patterns of Toll crystals grown in gel |
title | Liesegang-like patterns of Toll crystals grown in gel |
title_full | Liesegang-like patterns of Toll crystals grown in gel |
title_fullStr | Liesegang-like patterns of Toll crystals grown in gel |
title_full_unstemmed | Liesegang-like patterns of Toll crystals grown in gel |
title_short | Liesegang-like patterns of Toll crystals grown in gel |
title_sort | liesegang-like patterns of toll crystals grown in gel |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3627410/ https://www.ncbi.nlm.nih.gov/pubmed/23596340 http://dx.doi.org/10.1107/S0021889812051606 |
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