Cargando…

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated (Cas) systems in bacteria and archaea use RNA-guided nuclease activity to provide adaptive immunity against invading foreign nucleic acids. Here, we report the use of type II bacterial CRISPR-Cas system in Sacch...

Descripción completa

Detalles Bibliográficos
Autores principales:	DiCarlo, James E., Norville, Julie E., Mali, Prashant, Rios, Xavier, Aach, John, Church, George M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2013
Materias:	Synthetic Biology and Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3627607/ https://www.ncbi.nlm.nih.gov/pubmed/23460208 http://dx.doi.org/10.1093/nar/gkt135

Ejemplares similares

CRISPR-Cas: an efficient tool for genome engineering of virulent bacteriophages
por: Martel, Bruno, et al.
Publicado: (2014)

Efficient multiplexed gene regulation in Saccharomyces cerevisiae using dCas12a
por: Ciurkot, Klaudia, et al.
Publicado: (2021)

A Cas9-based toolkit to program gene expression in Saccharomyces cerevisiae
por: Reider Apel, Amanda, et al.
Publicado: (2017)

Rational optimization of tolC as a powerful dual selectable marker for genome engineering
por: Gregg, Christopher J., et al.
Publicado: (2014)

Utilizing RNA origami scaffolds in Saccharomyces cerevisiae for dCas9-mediated transcriptional control
por: Pothoulakis, Georgios, et al.
Publicado: (2022)

Metabolic engineering of Saccharomyces cerevisiae for hydroxytyrosol overproduction directly from glucose
por: Bisquert, Ricardo, et al.
Publicado: (2021)

CRISPR-mediated protein-tagging signal amplification systems for efficient transcriptional activation and repression in Saccharomyces cerevisiae
por: Zhai, Haotian, et al.
Publicado: (2022)

Streamlined CRISPR genome engineering in wild-type bacteria using SIBR-Cas
por: Patinios, Constantinos, et al.
Publicado: (2021)

Large chromosomal deletions and heritable small genetic changes induced by CRISPR/Cas9 in rice
por: Zhou, Huanbin, et al.
Publicado: (2014)

Production of S-methyl-methionine using engineered Saccharomyces cerevisiae sake K6
por: Lee, Jun-Min, et al.
Publicado: (2023)

Rational guide RNA engineering for small-molecule control of CRISPR/Cas9 and gene editing
por: Liu, Xingyu, et al.
Publicado: (2022)

Rational engineering of a modular bacterial CRISPR–Cas activation platform with expanded target range
por: Villegas Kcam, Maria Claudia, et al.
Publicado: (2021)

High-throughput antibody engineering in mammalian cells by CRISPR/Cas9-mediated homology-directed mutagenesis
por: Mason, Derek M, et al.
Publicado: (2018)

Characterization and repurposing of the endogenous Type I-F CRISPR–Cas system of Zymomonas mobilis for genome engineering
por: Zheng, Yanli, et al.
Publicado: (2019)

CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity
por: Cradick, Thomas J., et al.
Publicado: (2013)

CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences
por: Lin, Yanni, et al.
Publicado: (2014)

A supernumerary designer chromosome for modular in vivo pathway assembly in Saccharomyces cerevisiae
por: Postma, Eline D, et al.
Publicado: (2021)

FnCpf1: a novel and efficient genome editing tool for Saccharomyces cerevisiae
por: Świat, Michal A., et al.
Publicado: (2017)

Engineering acetyl-CoA supply and ERG9 repression to enhance mevalonate production in Saccharomyces cerevisiae
por: Wegner, Scott A, et al.
Publicado: (2021)

CRISPR–Cas12a system in fission yeast for multiplex genomic editing and CRISPR interference
por: Zhao, Yu, et al.
Publicado: (2020)

Unmodificated stepless regulation of CRISPR/Cas12a multi-performance
por: Zhao, Rong, et al.
Publicado: (2023)

Engineered lentivirus-derived nanoparticles (LVNPs) for delivery of CRISPR/Cas ribonucleoprotein complexes supporting base editing, prime editing and in vivo gene modification
por: Haldrup, Jakob, et al.
Publicado: (2023)

Repurposing endogenous type I CRISPR-Cas systems for programmable gene repression
por: Luo, Michelle L., et al.
Publicado: (2015)

A tightly regulated and adjustable CRISPR-dCas9 based AND gate in yeast
por: Hofmann, Anja, et al.
Publicado: (2019)

Engineering of temperature- and light-switchable Cas9 variants
por: Richter, Florian, et al.
Publicado: (2016)

Highly efficient rDNA‐mediated multicopy integration based on the dynamic balance of rDNA in Saccharomyces cerevisiae
por: Zheng, Huihui, et al.
Publicado: (2022)

Production of D-lactic acid containing polyhydroxyalkanoate polymers in yeast Saccharomyces cerevisiae
por: Ylinen, Anna, et al.
Publicado: (2021)

Toward a translationally independent RNA-based synthetic oscillator using deactivated CRISPR-Cas
por: Kuo, James, et al.
Publicado: (2020)

Enhancement of CRISPR/Cas12a trans-cleavage activity using hairpin DNA reporters
por: Rossetti, Marianna, et al.
Publicado: (2022)

CRISPR-associated type V proteins as a tool for controlling mRNA stability in S. cerevisiae synthetic gene circuits
por: Yu, Lifang, et al.
Publicado: (2023)

CRISPR-Cas9: A Powerful Tool to Efficiently Engineer Saccharomyces cerevisiae
por: Rainha, João, et al.
Publicado: (2020)

Modified bases enable high-efficiency oligonucleotide-mediated allelic replacement via mismatch repair evasion
por: Wang, Harris H., et al.
Publicado: (2011)

Efficient target cleavage by Type V Cas12a effectors programmed with split CRISPR RNA
por: Shebanova, Regina, et al.
Publicado: (2021)

CRISPR-RNAa: targeted activation of translation using dCas13 fusions to translation initiation factors
por: Otoupal, Peter B, et al.
Publicado: (2022)

Establishing artificial gene connections through RNA displacement–assembly-controlled CRISPR/Cas9 function
por: Wang, Wei-Jia, et al.
Publicado: (2023)

CRISPR/Cas9-mediated gene knockout is insensitive to target copy number but is dependent on guide RNA potency and Cas9/sgRNA threshold expression level
por: Yuen, Garmen, et al.
Publicado: (2017)

Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach
por: Chari, Raj, et al.
Publicado: (2015)

Targeted-antibacterial-plasmids (TAPs) combining conjugation and CRISPR/Cas systems achieve strain-specific antibacterial activity
por: Reuter, Audrey, et al.
Publicado: (2021)

An in-library ligation strategy and its application in CRISPR/Cas9 screening of high-order gRNA combinations
por: Lu, Zhike, et al.
Publicado: (2022)

G-quadruplex-guided RNA engineering to modulate CRISPR-based genomic regulation
por: Liu, Xingyu, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_p8ee14cukr5ut9uce3q580pj1f