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Prenatal Hypoxia Is Associated with Long-Term Retinal Dysfunction in Rats
BACKGROUND: Intra-uterine growth restriction (IUGR) has been associated with increased predisposition to age-related complications. We tested the hypothesis that rat offspring models of IUGR would exhibit exacerbated, age-related retinal dysfunction. METHODS: Female Sprague-Dawley rats (maintained a...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3627650/ https://www.ncbi.nlm.nih.gov/pubmed/23610595 http://dx.doi.org/10.1371/journal.pone.0061861 |
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author | Bourque, Stephane L. Kuny, Sharee Reyes, Laura M. Davidge, Sandra T. Sauvé, Yves |
author_facet | Bourque, Stephane L. Kuny, Sharee Reyes, Laura M. Davidge, Sandra T. Sauvé, Yves |
author_sort | Bourque, Stephane L. |
collection | PubMed |
description | BACKGROUND: Intra-uterine growth restriction (IUGR) has been associated with increased predisposition to age-related complications. We tested the hypothesis that rat offspring models of IUGR would exhibit exacerbated, age-related retinal dysfunction. METHODS: Female Sprague-Dawley rats (maintained at 11.5% O(2) from gestational day 15 to 21 to induce IUGR) and control offspring (maintained at 21% O(2) throughout pregnancy) had retinal function assessed at 2 months (young) and 14 months of age (aged) with electroretinogram (ERG) recordings. Retinal anatomy was assessed by immunofluorescence. RESULTS: Deficits in rod-driven retina function were observed in aged IUGR offspring, as evidenced by reduced amplitudes of dark-adapted mixed a-wave V(max) (by 49.3%, P<0.01), b-wave V(max) (by 42.1%, P<0.001) and dark-adapted peak oscillatory potentials (by 42.3%, P<0.01). In contrast to the rod-driven defects specific to aged IUGR offspring, light adapted ERG recordings revealed cone defects in young animals, that were stationary until old age. At 2 months, IUGR offspring had amplitude reductions for both b-wave (V(max) by 46%, P<0.01) and peak oscillatory potential (V(max) by 38%, P<0.05). Finally, defects in cone-driven responses were further confirmed by reduced maximal photopic flicker amplitudes at 2 (by 42%, P<0.001) and 14 months (by 34%, P = 0.06) and critical flicker fusion frequencies at 14 months (Control: 42±1 Hz, IUGR: 35±2 Hz, P<0.05). These functional changes were not paralleled by anatomical losses in IUGR offspring retinas. CONCLUSIONS: These data support that the developing retina is sensitive to stressors, and that pathways governing cone- and rod-driven function differ in their susceptibilities. In the case of prenatal hypoxia, cone- and rod-driven dysfunction manifest at young and old ages, respectively. We must, therefore, take into account the specific impact that fetal programming might exert on age-related retinal dystrophies when considering related diagnoses and therapeutic applications. |
format | Online Article Text |
id | pubmed-3627650 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36276502013-04-22 Prenatal Hypoxia Is Associated with Long-Term Retinal Dysfunction in Rats Bourque, Stephane L. Kuny, Sharee Reyes, Laura M. Davidge, Sandra T. Sauvé, Yves PLoS One Research Article BACKGROUND: Intra-uterine growth restriction (IUGR) has been associated with increased predisposition to age-related complications. We tested the hypothesis that rat offspring models of IUGR would exhibit exacerbated, age-related retinal dysfunction. METHODS: Female Sprague-Dawley rats (maintained at 11.5% O(2) from gestational day 15 to 21 to induce IUGR) and control offspring (maintained at 21% O(2) throughout pregnancy) had retinal function assessed at 2 months (young) and 14 months of age (aged) with electroretinogram (ERG) recordings. Retinal anatomy was assessed by immunofluorescence. RESULTS: Deficits in rod-driven retina function were observed in aged IUGR offspring, as evidenced by reduced amplitudes of dark-adapted mixed a-wave V(max) (by 49.3%, P<0.01), b-wave V(max) (by 42.1%, P<0.001) and dark-adapted peak oscillatory potentials (by 42.3%, P<0.01). In contrast to the rod-driven defects specific to aged IUGR offspring, light adapted ERG recordings revealed cone defects in young animals, that were stationary until old age. At 2 months, IUGR offspring had amplitude reductions for both b-wave (V(max) by 46%, P<0.01) and peak oscillatory potential (V(max) by 38%, P<0.05). Finally, defects in cone-driven responses were further confirmed by reduced maximal photopic flicker amplitudes at 2 (by 42%, P<0.001) and 14 months (by 34%, P = 0.06) and critical flicker fusion frequencies at 14 months (Control: 42±1 Hz, IUGR: 35±2 Hz, P<0.05). These functional changes were not paralleled by anatomical losses in IUGR offspring retinas. CONCLUSIONS: These data support that the developing retina is sensitive to stressors, and that pathways governing cone- and rod-driven function differ in their susceptibilities. In the case of prenatal hypoxia, cone- and rod-driven dysfunction manifest at young and old ages, respectively. We must, therefore, take into account the specific impact that fetal programming might exert on age-related retinal dystrophies when considering related diagnoses and therapeutic applications. Public Library of Science 2013-04-16 /pmc/articles/PMC3627650/ /pubmed/23610595 http://dx.doi.org/10.1371/journal.pone.0061861 Text en © 2013 Bourque et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Bourque, Stephane L. Kuny, Sharee Reyes, Laura M. Davidge, Sandra T. Sauvé, Yves Prenatal Hypoxia Is Associated with Long-Term Retinal Dysfunction in Rats |
title | Prenatal Hypoxia Is Associated with Long-Term Retinal Dysfunction in Rats |
title_full | Prenatal Hypoxia Is Associated with Long-Term Retinal Dysfunction in Rats |
title_fullStr | Prenatal Hypoxia Is Associated with Long-Term Retinal Dysfunction in Rats |
title_full_unstemmed | Prenatal Hypoxia Is Associated with Long-Term Retinal Dysfunction in Rats |
title_short | Prenatal Hypoxia Is Associated with Long-Term Retinal Dysfunction in Rats |
title_sort | prenatal hypoxia is associated with long-term retinal dysfunction in rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3627650/ https://www.ncbi.nlm.nih.gov/pubmed/23610595 http://dx.doi.org/10.1371/journal.pone.0061861 |
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