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Effects of nerve growth factor on the action potential duration and repolarizing currents in a rabbit model of myocardial infarction

OBJECTIVES: To investigate the effect of nerve growth factor (NGF) on the action potential and potassium currents of non-infarcted myocardium in the myocardial infarcted rabbit model. METHODS: Rabbits with occlusion of the left anterior descending coronary artery were prepared and allowed to recover...

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Autores principales: Lan, Yun-Feng, Zhang, Jian-Cheng, Gao, Jin-Lao, Wang, Xue-Ping, Fang, Zhou, Fu, Yi-Cheng, Chen, Mei-Yan, Lin, Min, Xue, Qiao, Li, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Science Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3627714/
https://www.ncbi.nlm.nih.gov/pubmed/23610573
http://dx.doi.org/10.3969/j.issn.1671-5411.2013.01.008
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author Lan, Yun-Feng
Zhang, Jian-Cheng
Gao, Jin-Lao
Wang, Xue-Ping
Fang, Zhou
Fu, Yi-Cheng
Chen, Mei-Yan
Lin, Min
Xue, Qiao
Li, Yang
author_facet Lan, Yun-Feng
Zhang, Jian-Cheng
Gao, Jin-Lao
Wang, Xue-Ping
Fang, Zhou
Fu, Yi-Cheng
Chen, Mei-Yan
Lin, Min
Xue, Qiao
Li, Yang
author_sort Lan, Yun-Feng
collection PubMed
description OBJECTIVES: To investigate the effect of nerve growth factor (NGF) on the action potential and potassium currents of non-infarcted myocardium in the myocardial infarcted rabbit model. METHODS: Rabbits with occlusion of the left anterior descending coronary artery were prepared and allowed to recover for eight weeks (healed myocardial infarction, HMI). During ligation surgery of the left coronary artery, a polyethylene tube was placed near the left stellate ganglion in the subcutis of the neck for the purpose of administering NGF 400 U/d for eight weeks (HMI + NGF group). Cardiomyocytes were isolated from regions of the non-infarcted left ventricular wall and the action potentials and ion currents in these cells were recorded using whole-cell patch clamps. RESULTS: Compared with HMI and control cardiomyocytes, significant prolongation of APD(50) or APD(90) (Action potential duration (APD) measured at 50% and 90% of repolarization) in HMI + NGF cardiomyocytes was found. The results showed that the 4-aminopyridine sensitive transient outward potassium current (I(to)), the rapidly activated omponent of delayed rectifier potassium current (I(Kr)), the slowly activated component of delayed rectifier potassium current (I(Ks)), and the L-type calcium current (I(CaL)) were significantly altered in NGF + HMI cardiomyocytes compared with HMI and control cells. CONCLUSIONS: Our results suggest that NGF treatment significantly prolongs APD in HMI cardiomyocytes and that a decrease in outward potassium currents and an increase of inward Ca(2+) current are likely the underlying mechanism of action.
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spelling pubmed-36277142013-04-22 Effects of nerve growth factor on the action potential duration and repolarizing currents in a rabbit model of myocardial infarction Lan, Yun-Feng Zhang, Jian-Cheng Gao, Jin-Lao Wang, Xue-Ping Fang, Zhou Fu, Yi-Cheng Chen, Mei-Yan Lin, Min Xue, Qiao Li, Yang J Geriatr Cardiol Research Article OBJECTIVES: To investigate the effect of nerve growth factor (NGF) on the action potential and potassium currents of non-infarcted myocardium in the myocardial infarcted rabbit model. METHODS: Rabbits with occlusion of the left anterior descending coronary artery were prepared and allowed to recover for eight weeks (healed myocardial infarction, HMI). During ligation surgery of the left coronary artery, a polyethylene tube was placed near the left stellate ganglion in the subcutis of the neck for the purpose of administering NGF 400 U/d for eight weeks (HMI + NGF group). Cardiomyocytes were isolated from regions of the non-infarcted left ventricular wall and the action potentials and ion currents in these cells were recorded using whole-cell patch clamps. RESULTS: Compared with HMI and control cardiomyocytes, significant prolongation of APD(50) or APD(90) (Action potential duration (APD) measured at 50% and 90% of repolarization) in HMI + NGF cardiomyocytes was found. The results showed that the 4-aminopyridine sensitive transient outward potassium current (I(to)), the rapidly activated omponent of delayed rectifier potassium current (I(Kr)), the slowly activated component of delayed rectifier potassium current (I(Ks)), and the L-type calcium current (I(CaL)) were significantly altered in NGF + HMI cardiomyocytes compared with HMI and control cells. CONCLUSIONS: Our results suggest that NGF treatment significantly prolongs APD in HMI cardiomyocytes and that a decrease in outward potassium currents and an increase of inward Ca(2+) current are likely the underlying mechanism of action. Science Press 2013-03 /pmc/articles/PMC3627714/ /pubmed/23610573 http://dx.doi.org/10.3969/j.issn.1671-5411.2013.01.008 Text en Institute of Geriatric Cardiology http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License, which allows readers to alter, transform, or build upon the article and then distribute the resulting work under the same or similar license to this one. The work must be attributed back to the original author and commercial use is not permitted without specific permission.
spellingShingle Research Article
Lan, Yun-Feng
Zhang, Jian-Cheng
Gao, Jin-Lao
Wang, Xue-Ping
Fang, Zhou
Fu, Yi-Cheng
Chen, Mei-Yan
Lin, Min
Xue, Qiao
Li, Yang
Effects of nerve growth factor on the action potential duration and repolarizing currents in a rabbit model of myocardial infarction
title Effects of nerve growth factor on the action potential duration and repolarizing currents in a rabbit model of myocardial infarction
title_full Effects of nerve growth factor on the action potential duration and repolarizing currents in a rabbit model of myocardial infarction
title_fullStr Effects of nerve growth factor on the action potential duration and repolarizing currents in a rabbit model of myocardial infarction
title_full_unstemmed Effects of nerve growth factor on the action potential duration and repolarizing currents in a rabbit model of myocardial infarction
title_short Effects of nerve growth factor on the action potential duration and repolarizing currents in a rabbit model of myocardial infarction
title_sort effects of nerve growth factor on the action potential duration and repolarizing currents in a rabbit model of myocardial infarction
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3627714/
https://www.ncbi.nlm.nih.gov/pubmed/23610573
http://dx.doi.org/10.3969/j.issn.1671-5411.2013.01.008
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