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Whole genome sequencing identifies zoonotic transmission of MRSA isolates with the novel mecA homologue mecC

Several methicillin-resistant Staphylococcus aureus (MRSA) lineages that carry a novel mecA homologue (mecC) have recently been described in livestock and humans. In Denmark, two independent human cases of mecC-MRSA infection have been linked to a livestock reservoir. We investigated the molecular e...

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Autores principales: Harrison, Ewan M, Paterson, Gavin K, Holden, Matthew TG, Larsen, Jesper, Stegger, Marc, Larsen, Anders Rhod, Petersen, Andreas, Skov, Robert L, Christensen, Judit Marta, Bak Zeuthen, Anne, Heltberg, Ole, Harris, Simon R, Zadoks, Ruth N, Parkhill, Julian, Peacock, Sharon J, Holmes, Mark A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: WILEY-VCH Verlag 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3628104/
https://www.ncbi.nlm.nih.gov/pubmed/23526809
http://dx.doi.org/10.1002/emmm.201202413
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author Harrison, Ewan M
Paterson, Gavin K
Holden, Matthew TG
Larsen, Jesper
Stegger, Marc
Larsen, Anders Rhod
Petersen, Andreas
Skov, Robert L
Christensen, Judit Marta
Bak Zeuthen, Anne
Heltberg, Ole
Harris, Simon R
Zadoks, Ruth N
Parkhill, Julian
Peacock, Sharon J
Holmes, Mark A
author_facet Harrison, Ewan M
Paterson, Gavin K
Holden, Matthew TG
Larsen, Jesper
Stegger, Marc
Larsen, Anders Rhod
Petersen, Andreas
Skov, Robert L
Christensen, Judit Marta
Bak Zeuthen, Anne
Heltberg, Ole
Harris, Simon R
Zadoks, Ruth N
Parkhill, Julian
Peacock, Sharon J
Holmes, Mark A
author_sort Harrison, Ewan M
collection PubMed
description Several methicillin-resistant Staphylococcus aureus (MRSA) lineages that carry a novel mecA homologue (mecC) have recently been described in livestock and humans. In Denmark, two independent human cases of mecC-MRSA infection have been linked to a livestock reservoir. We investigated the molecular epidemiology of the associated MRSA isolates using whole genome sequencing (WGS). Single nucleotide polymorphisms (SNP) were defined and compared to a reference genome to place the isolates into a phylogenetic context. Phylogenetic analysis revealed two distinct farm-specific clusters comprising isolates from the human case and their own livestock, whereas human and animal isolates from the same farm only differed by a small number of SNPs, which supports the likelihood of zoonotic transmission. Further analyses identified a number of genes and mutations that may be associated with host interaction and virulence. This study demonstrates that mecC-MRSA ST130 isolates are capable of transmission between animals and humans, and underscores the potential of WGS in epidemiological investigations and source tracking of bacterial infections.
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spelling pubmed-36281042013-04-19 Whole genome sequencing identifies zoonotic transmission of MRSA isolates with the novel mecA homologue mecC Harrison, Ewan M Paterson, Gavin K Holden, Matthew TG Larsen, Jesper Stegger, Marc Larsen, Anders Rhod Petersen, Andreas Skov, Robert L Christensen, Judit Marta Bak Zeuthen, Anne Heltberg, Ole Harris, Simon R Zadoks, Ruth N Parkhill, Julian Peacock, Sharon J Holmes, Mark A EMBO Mol Med Report Several methicillin-resistant Staphylococcus aureus (MRSA) lineages that carry a novel mecA homologue (mecC) have recently been described in livestock and humans. In Denmark, two independent human cases of mecC-MRSA infection have been linked to a livestock reservoir. We investigated the molecular epidemiology of the associated MRSA isolates using whole genome sequencing (WGS). Single nucleotide polymorphisms (SNP) were defined and compared to a reference genome to place the isolates into a phylogenetic context. Phylogenetic analysis revealed two distinct farm-specific clusters comprising isolates from the human case and their own livestock, whereas human and animal isolates from the same farm only differed by a small number of SNPs, which supports the likelihood of zoonotic transmission. Further analyses identified a number of genes and mutations that may be associated with host interaction and virulence. This study demonstrates that mecC-MRSA ST130 isolates are capable of transmission between animals and humans, and underscores the potential of WGS in epidemiological investigations and source tracking of bacterial infections. WILEY-VCH Verlag 2013-04 2013-03-25 /pmc/articles/PMC3628104/ /pubmed/23526809 http://dx.doi.org/10.1002/emmm.201202413 Text en Copyright © 2013 The Authors. Published by John Wiley and Sons, Ltd on behalf of EMBO http://creativecommons.org/licenses/by/2.5/ This is an open access article under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Report
Harrison, Ewan M
Paterson, Gavin K
Holden, Matthew TG
Larsen, Jesper
Stegger, Marc
Larsen, Anders Rhod
Petersen, Andreas
Skov, Robert L
Christensen, Judit Marta
Bak Zeuthen, Anne
Heltberg, Ole
Harris, Simon R
Zadoks, Ruth N
Parkhill, Julian
Peacock, Sharon J
Holmes, Mark A
Whole genome sequencing identifies zoonotic transmission of MRSA isolates with the novel mecA homologue mecC
title Whole genome sequencing identifies zoonotic transmission of MRSA isolates with the novel mecA homologue mecC
title_full Whole genome sequencing identifies zoonotic transmission of MRSA isolates with the novel mecA homologue mecC
title_fullStr Whole genome sequencing identifies zoonotic transmission of MRSA isolates with the novel mecA homologue mecC
title_full_unstemmed Whole genome sequencing identifies zoonotic transmission of MRSA isolates with the novel mecA homologue mecC
title_short Whole genome sequencing identifies zoonotic transmission of MRSA isolates with the novel mecA homologue mecC
title_sort whole genome sequencing identifies zoonotic transmission of mrsa isolates with the novel meca homologue mecc
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3628104/
https://www.ncbi.nlm.nih.gov/pubmed/23526809
http://dx.doi.org/10.1002/emmm.201202413
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