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Xenopus oocyte meiosis lacks spindle assembly checkpoint control
The spindle assembly checkpoint (SAC) functions as a surveillance mechanism to detect chromosome misalignment and to delay anaphase until the errors are corrected. The SAC is thought to control mitosis and meiosis, including meiosis in mammalian eggs. However, it remains unknown if meiosis in the eg...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3628510/ https://www.ncbi.nlm.nih.gov/pubmed/23569212 http://dx.doi.org/10.1083/jcb.201211041 |
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author | Shao, Hua Li, Ruizhen Ma, Chunqi Chen, Eric Liu, X. Johné |
author_facet | Shao, Hua Li, Ruizhen Ma, Chunqi Chen, Eric Liu, X. Johné |
author_sort | Shao, Hua |
collection | PubMed |
description | The spindle assembly checkpoint (SAC) functions as a surveillance mechanism to detect chromosome misalignment and to delay anaphase until the errors are corrected. The SAC is thought to control mitosis and meiosis, including meiosis in mammalian eggs. However, it remains unknown if meiosis in the eggs of nonmammalian vertebrate species is also regulated by SAC. Using a novel karyotyping technique, we demonstrate that complete disruption of spindle microtubules in Xenopus laevis oocytes did not affect the bivalent-to-dyad transition at the time oocytes are undergoing anaphase I. These oocytes also acquired the ability to respond to parthenogenetic activation, which indicates proper metaphase II arrest. Similarly, oocytes exhibiting monopolar spindles, via inhibition of aurora B or Eg5 kinesin, underwent monopolar anaphase on time and without additional intervention. Therefore, the metaphase-to-anaphase transition in frog oocytes is not regulated by SAC. |
format | Online Article Text |
id | pubmed-3628510 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-36285102013-10-15 Xenopus oocyte meiosis lacks spindle assembly checkpoint control Shao, Hua Li, Ruizhen Ma, Chunqi Chen, Eric Liu, X. Johné J Cell Biol Research Articles The spindle assembly checkpoint (SAC) functions as a surveillance mechanism to detect chromosome misalignment and to delay anaphase until the errors are corrected. The SAC is thought to control mitosis and meiosis, including meiosis in mammalian eggs. However, it remains unknown if meiosis in the eggs of nonmammalian vertebrate species is also regulated by SAC. Using a novel karyotyping technique, we demonstrate that complete disruption of spindle microtubules in Xenopus laevis oocytes did not affect the bivalent-to-dyad transition at the time oocytes are undergoing anaphase I. These oocytes also acquired the ability to respond to parthenogenetic activation, which indicates proper metaphase II arrest. Similarly, oocytes exhibiting monopolar spindles, via inhibition of aurora B or Eg5 kinesin, underwent monopolar anaphase on time and without additional intervention. Therefore, the metaphase-to-anaphase transition in frog oocytes is not regulated by SAC. The Rockefeller University Press 2013-04-15 /pmc/articles/PMC3628510/ /pubmed/23569212 http://dx.doi.org/10.1083/jcb.201211041 Text en © 2013 Shao et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Shao, Hua Li, Ruizhen Ma, Chunqi Chen, Eric Liu, X. Johné Xenopus oocyte meiosis lacks spindle assembly checkpoint control |
title | Xenopus oocyte meiosis lacks spindle assembly checkpoint control |
title_full | Xenopus oocyte meiosis lacks spindle assembly checkpoint control |
title_fullStr | Xenopus oocyte meiosis lacks spindle assembly checkpoint control |
title_full_unstemmed | Xenopus oocyte meiosis lacks spindle assembly checkpoint control |
title_short | Xenopus oocyte meiosis lacks spindle assembly checkpoint control |
title_sort | xenopus oocyte meiosis lacks spindle assembly checkpoint control |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3628510/ https://www.ncbi.nlm.nih.gov/pubmed/23569212 http://dx.doi.org/10.1083/jcb.201211041 |
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