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Simultaneous Quantitative Live Cell Imaging of Multiple FRET-Based Biosensors
We have developed a novel method for multi-color spectral FRET analysis which is used to study a system of three independent FRET-based molecular sensors composed of the combinations of only three fluorescent proteins. This method is made possible by a novel routine for computing the 3-D excitation/...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2013
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3628763/ https://www.ncbi.nlm.nih.gov/pubmed/23613792 http://dx.doi.org/10.1371/journal.pone.0061096 |