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Identification of miR-27b as a Novel Signature from the mRNA Profiles of Adipose-Derived Mesenchymal Stem Cells Involved in the Tolerogenic Response

Adipose-derived mesenchymal stem cells (adipose-derived MSCs, ASCs) possess the ability to differentiate into multiple tissue types and have immune-modulatory properties similar to those of MSCs from other origins. However, the regulation of the MSC-elicited immune-modulatory activity by specific mi...

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Autores principales: Chen, Kuang-Den, Goto, Shigeru, Hsu, Li-Wen, Lin, Tzu-Yang, Nakano, Toshiaki, Lai, Chia-Yun, Chang, Yen-Chen, Weng, Wei-Teng, Kuo, Yur-Ren, Wang, Chih-Chi, Cheng, Yu-Fan, Ma, Yen-Ying, Lin, Chih-Che, Chen, Chao-Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3628792/
https://www.ncbi.nlm.nih.gov/pubmed/23613728
http://dx.doi.org/10.1371/journal.pone.0060492
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author Chen, Kuang-Den
Goto, Shigeru
Hsu, Li-Wen
Lin, Tzu-Yang
Nakano, Toshiaki
Lai, Chia-Yun
Chang, Yen-Chen
Weng, Wei-Teng
Kuo, Yur-Ren
Wang, Chih-Chi
Cheng, Yu-Fan
Ma, Yen-Ying
Lin, Chih-Che
Chen, Chao-Long
author_facet Chen, Kuang-Den
Goto, Shigeru
Hsu, Li-Wen
Lin, Tzu-Yang
Nakano, Toshiaki
Lai, Chia-Yun
Chang, Yen-Chen
Weng, Wei-Teng
Kuo, Yur-Ren
Wang, Chih-Chi
Cheng, Yu-Fan
Ma, Yen-Ying
Lin, Chih-Che
Chen, Chao-Long
author_sort Chen, Kuang-Den
collection PubMed
description Adipose-derived mesenchymal stem cells (adipose-derived MSCs, ASCs) possess the ability to differentiate into multiple tissue types and have immune-modulatory properties similar to those of MSCs from other origins. However, the regulation of the MSC-elicited immune-modulatory activity by specific microRNA (miRNA) mechanisms remains unexplored. Gene expression profiling with knowledge-based functional enrichment analysis is an appropriate approach for unraveling these mechanisms. This tool can be used to examine the transcripts and miRNA regulators that differentiate the rat tolerogenic orthotopic liver transplantation (OLT; DA liver into PVG) and rejection OLT (DA liver into LEW) models. In both models, the rejection reaction was observed on postoperative day 7∼14 (rejection phase) but was overcome only by the PVG recipients. Thus, the global gene expression patterns of ASCs from spontaneously tolerant (PVG) and acute rejecting (LEW) rats in response to LPS activation were compared. In this study, we performed miRNA enrichment analysis based on the analysis of pathway, gene ontology (GO) terms and transcription factor binding site (TFBS) motif annotations. We found that the top candidate, miR-27, was specifically enriched and had the highest predicted frequency. We also identified a greater than 3-fold increase of miR-27b expression in the ASCs of tolerant recipients (DA to PVG) compared to those of rejecting recipients (DA to LEW) during the rejection phase in the rat OLT model. Furthermore, our data showed that miR-27b knockdown has a positive influence on the allosuppressive activity that inhibits T-cell proliferation. We found that miR-27 knockdown significantly induced the expression of CXCL12 in cultured ASCs and the expression of CXCL12 was responsible for the miR-27b antagomir-mediated inhibition of T-cell proliferation. These results, which through a series of comprehensive miRNA enrichment analyses, might be relevant for stem cell-based therapeutic applications in immunosuppressive function using ASCs.
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spelling pubmed-36287922013-04-23 Identification of miR-27b as a Novel Signature from the mRNA Profiles of Adipose-Derived Mesenchymal Stem Cells Involved in the Tolerogenic Response Chen, Kuang-Den Goto, Shigeru Hsu, Li-Wen Lin, Tzu-Yang Nakano, Toshiaki Lai, Chia-Yun Chang, Yen-Chen Weng, Wei-Teng Kuo, Yur-Ren Wang, Chih-Chi Cheng, Yu-Fan Ma, Yen-Ying Lin, Chih-Che Chen, Chao-Long PLoS One Research Article Adipose-derived mesenchymal stem cells (adipose-derived MSCs, ASCs) possess the ability to differentiate into multiple tissue types and have immune-modulatory properties similar to those of MSCs from other origins. However, the regulation of the MSC-elicited immune-modulatory activity by specific microRNA (miRNA) mechanisms remains unexplored. Gene expression profiling with knowledge-based functional enrichment analysis is an appropriate approach for unraveling these mechanisms. This tool can be used to examine the transcripts and miRNA regulators that differentiate the rat tolerogenic orthotopic liver transplantation (OLT; DA liver into PVG) and rejection OLT (DA liver into LEW) models. In both models, the rejection reaction was observed on postoperative day 7∼14 (rejection phase) but was overcome only by the PVG recipients. Thus, the global gene expression patterns of ASCs from spontaneously tolerant (PVG) and acute rejecting (LEW) rats in response to LPS activation were compared. In this study, we performed miRNA enrichment analysis based on the analysis of pathway, gene ontology (GO) terms and transcription factor binding site (TFBS) motif annotations. We found that the top candidate, miR-27, was specifically enriched and had the highest predicted frequency. We also identified a greater than 3-fold increase of miR-27b expression in the ASCs of tolerant recipients (DA to PVG) compared to those of rejecting recipients (DA to LEW) during the rejection phase in the rat OLT model. Furthermore, our data showed that miR-27b knockdown has a positive influence on the allosuppressive activity that inhibits T-cell proliferation. We found that miR-27 knockdown significantly induced the expression of CXCL12 in cultured ASCs and the expression of CXCL12 was responsible for the miR-27b antagomir-mediated inhibition of T-cell proliferation. These results, which through a series of comprehensive miRNA enrichment analyses, might be relevant for stem cell-based therapeutic applications in immunosuppressive function using ASCs. Public Library of Science 2013-04-16 /pmc/articles/PMC3628792/ /pubmed/23613728 http://dx.doi.org/10.1371/journal.pone.0060492 Text en © 2013 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Chen, Kuang-Den
Goto, Shigeru
Hsu, Li-Wen
Lin, Tzu-Yang
Nakano, Toshiaki
Lai, Chia-Yun
Chang, Yen-Chen
Weng, Wei-Teng
Kuo, Yur-Ren
Wang, Chih-Chi
Cheng, Yu-Fan
Ma, Yen-Ying
Lin, Chih-Che
Chen, Chao-Long
Identification of miR-27b as a Novel Signature from the mRNA Profiles of Adipose-Derived Mesenchymal Stem Cells Involved in the Tolerogenic Response
title Identification of miR-27b as a Novel Signature from the mRNA Profiles of Adipose-Derived Mesenchymal Stem Cells Involved in the Tolerogenic Response
title_full Identification of miR-27b as a Novel Signature from the mRNA Profiles of Adipose-Derived Mesenchymal Stem Cells Involved in the Tolerogenic Response
title_fullStr Identification of miR-27b as a Novel Signature from the mRNA Profiles of Adipose-Derived Mesenchymal Stem Cells Involved in the Tolerogenic Response
title_full_unstemmed Identification of miR-27b as a Novel Signature from the mRNA Profiles of Adipose-Derived Mesenchymal Stem Cells Involved in the Tolerogenic Response
title_short Identification of miR-27b as a Novel Signature from the mRNA Profiles of Adipose-Derived Mesenchymal Stem Cells Involved in the Tolerogenic Response
title_sort identification of mir-27b as a novel signature from the mrna profiles of adipose-derived mesenchymal stem cells involved in the tolerogenic response
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3628792/
https://www.ncbi.nlm.nih.gov/pubmed/23613728
http://dx.doi.org/10.1371/journal.pone.0060492
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