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Defining the Genomic Signature of Totipotency and Pluripotency during Early Human Development

The genetic mechanisms governing human pre-implantation embryo development and the in vitro counterparts, human embryonic stem cells (hESCs), still remain incomplete. Previous global genome studies demonstrated that totipotent blastomeres from day-3 human embryos and pluripotent inner cell masses (I...

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Autores principales: Galan, Amparo, Diaz-Gimeno, Patricia, Poo, Maria Eugenia, Valbuena, Diana, Sanchez, Eva, Ruiz, Veronica, Dopazo, Joaquin, Montaner, David, Conesa, Ana, Simon, Carlos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3629124/
https://www.ncbi.nlm.nih.gov/pubmed/23614026
http://dx.doi.org/10.1371/journal.pone.0062135
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author Galan, Amparo
Diaz-Gimeno, Patricia
Poo, Maria Eugenia
Valbuena, Diana
Sanchez, Eva
Ruiz, Veronica
Dopazo, Joaquin
Montaner, David
Conesa, Ana
Simon, Carlos
author_facet Galan, Amparo
Diaz-Gimeno, Patricia
Poo, Maria Eugenia
Valbuena, Diana
Sanchez, Eva
Ruiz, Veronica
Dopazo, Joaquin
Montaner, David
Conesa, Ana
Simon, Carlos
author_sort Galan, Amparo
collection PubMed
description The genetic mechanisms governing human pre-implantation embryo development and the in vitro counterparts, human embryonic stem cells (hESCs), still remain incomplete. Previous global genome studies demonstrated that totipotent blastomeres from day-3 human embryos and pluripotent inner cell masses (ICMs) from blastocysts, display unique and differing transcriptomes. Nevertheless, comparative gene expression analysis has revealed that no significant differences exist between hESCs derived from blastomeres versus those obtained from ICMs, suggesting that pluripotent hESCs involve a new developmental progression. To understand early human stages evolution, we developed an undifferentiation network signature (UNS) and applied it to a differential gene expression profile between single blastomeres from day-3 embryos, ICMs and hESCs. This allowed us to establish a unique signature composed of highly interconnected genes characteristic of totipotency (61 genes), in vivo pluripotency (20 genes), and in vitro pluripotency (107 genes), and which are also proprietary according to functional analysis. This systems biology approach has led to an improved understanding of the molecular and signaling processes governing human pre-implantation embryo development, as well as enabling us to comprehend how hESCs might adapt to in vitro culture conditions.
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spelling pubmed-36291242013-04-23 Defining the Genomic Signature of Totipotency and Pluripotency during Early Human Development Galan, Amparo Diaz-Gimeno, Patricia Poo, Maria Eugenia Valbuena, Diana Sanchez, Eva Ruiz, Veronica Dopazo, Joaquin Montaner, David Conesa, Ana Simon, Carlos PLoS One Research Article The genetic mechanisms governing human pre-implantation embryo development and the in vitro counterparts, human embryonic stem cells (hESCs), still remain incomplete. Previous global genome studies demonstrated that totipotent blastomeres from day-3 human embryos and pluripotent inner cell masses (ICMs) from blastocysts, display unique and differing transcriptomes. Nevertheless, comparative gene expression analysis has revealed that no significant differences exist between hESCs derived from blastomeres versus those obtained from ICMs, suggesting that pluripotent hESCs involve a new developmental progression. To understand early human stages evolution, we developed an undifferentiation network signature (UNS) and applied it to a differential gene expression profile between single blastomeres from day-3 embryos, ICMs and hESCs. This allowed us to establish a unique signature composed of highly interconnected genes characteristic of totipotency (61 genes), in vivo pluripotency (20 genes), and in vitro pluripotency (107 genes), and which are also proprietary according to functional analysis. This systems biology approach has led to an improved understanding of the molecular and signaling processes governing human pre-implantation embryo development, as well as enabling us to comprehend how hESCs might adapt to in vitro culture conditions. Public Library of Science 2013-04-17 /pmc/articles/PMC3629124/ /pubmed/23614026 http://dx.doi.org/10.1371/journal.pone.0062135 Text en © 2013 Galan et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Galan, Amparo
Diaz-Gimeno, Patricia
Poo, Maria Eugenia
Valbuena, Diana
Sanchez, Eva
Ruiz, Veronica
Dopazo, Joaquin
Montaner, David
Conesa, Ana
Simon, Carlos
Defining the Genomic Signature of Totipotency and Pluripotency during Early Human Development
title Defining the Genomic Signature of Totipotency and Pluripotency during Early Human Development
title_full Defining the Genomic Signature of Totipotency and Pluripotency during Early Human Development
title_fullStr Defining the Genomic Signature of Totipotency and Pluripotency during Early Human Development
title_full_unstemmed Defining the Genomic Signature of Totipotency and Pluripotency during Early Human Development
title_short Defining the Genomic Signature of Totipotency and Pluripotency during Early Human Development
title_sort defining the genomic signature of totipotency and pluripotency during early human development
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3629124/
https://www.ncbi.nlm.nih.gov/pubmed/23614026
http://dx.doi.org/10.1371/journal.pone.0062135
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