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High concentrations of cellulosic ethanol achieved by fed batch semi simultaneous saccharification and fermentation of waste-paper

A fundamental goal of second generation ethanol production is to increase the ethanol concentration to 10% (v/v) or more to optimise distillation costs. Semi simultaneous saccharification and fermentations (SSSF) were conducted at small pilot scale (5 L) utilising fed-batch additions of solid shredd...

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Autores principales: Elliston, Adam, Collins, Samuel R.A., Wilson, David R., Roberts, Ian N., Waldron, Keith W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Applied Science ;, Elsevier Science Pub. Co 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3629557/
https://www.ncbi.nlm.nih.gov/pubmed/23500568
http://dx.doi.org/10.1016/j.biortech.2013.01.084
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author Elliston, Adam
Collins, Samuel R.A.
Wilson, David R.
Roberts, Ian N.
Waldron, Keith W.
author_facet Elliston, Adam
Collins, Samuel R.A.
Wilson, David R.
Roberts, Ian N.
Waldron, Keith W.
author_sort Elliston, Adam
collection PubMed
description A fundamental goal of second generation ethanol production is to increase the ethanol concentration to 10% (v/v) or more to optimise distillation costs. Semi simultaneous saccharification and fermentations (SSSF) were conducted at small pilot scale (5 L) utilising fed-batch additions of solid shredded copier paper substrate. Early addition of Accellerase® 1500 at 16 FPU/g substrate and 30 U/g β-glucosidase followed by substrate only batch addition allowed low final equivalent enzyme concentrations to be achieved (3.7 FPU/g substrate) whilst maintaining digestion. Batch addition resulted in a cumulative substrate concentration equivalent to 65% (w/v). This in turn resulted in the production of high concentrations of ethanol (11.6% v/v). The success of this strategy relied on the capacity of the bioreactor to perform high shear mixing as required. Further research into the timing and number of substrate additions could lead to further improvement in overall yields from the 65.5% attained.
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spelling pubmed-36295572013-04-18 High concentrations of cellulosic ethanol achieved by fed batch semi simultaneous saccharification and fermentation of waste-paper Elliston, Adam Collins, Samuel R.A. Wilson, David R. Roberts, Ian N. Waldron, Keith W. Bioresour Technol Article A fundamental goal of second generation ethanol production is to increase the ethanol concentration to 10% (v/v) or more to optimise distillation costs. Semi simultaneous saccharification and fermentations (SSSF) were conducted at small pilot scale (5 L) utilising fed-batch additions of solid shredded copier paper substrate. Early addition of Accellerase® 1500 at 16 FPU/g substrate and 30 U/g β-glucosidase followed by substrate only batch addition allowed low final equivalent enzyme concentrations to be achieved (3.7 FPU/g substrate) whilst maintaining digestion. Batch addition resulted in a cumulative substrate concentration equivalent to 65% (w/v). This in turn resulted in the production of high concentrations of ethanol (11.6% v/v). The success of this strategy relied on the capacity of the bioreactor to perform high shear mixing as required. Further research into the timing and number of substrate additions could lead to further improvement in overall yields from the 65.5% attained. Elsevier Applied Science ;, Elsevier Science Pub. Co 2013-04 /pmc/articles/PMC3629557/ /pubmed/23500568 http://dx.doi.org/10.1016/j.biortech.2013.01.084 Text en © 2013 Elsevier Ltd. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Article
Elliston, Adam
Collins, Samuel R.A.
Wilson, David R.
Roberts, Ian N.
Waldron, Keith W.
High concentrations of cellulosic ethanol achieved by fed batch semi simultaneous saccharification and fermentation of waste-paper
title High concentrations of cellulosic ethanol achieved by fed batch semi simultaneous saccharification and fermentation of waste-paper
title_full High concentrations of cellulosic ethanol achieved by fed batch semi simultaneous saccharification and fermentation of waste-paper
title_fullStr High concentrations of cellulosic ethanol achieved by fed batch semi simultaneous saccharification and fermentation of waste-paper
title_full_unstemmed High concentrations of cellulosic ethanol achieved by fed batch semi simultaneous saccharification and fermentation of waste-paper
title_short High concentrations of cellulosic ethanol achieved by fed batch semi simultaneous saccharification and fermentation of waste-paper
title_sort high concentrations of cellulosic ethanol achieved by fed batch semi simultaneous saccharification and fermentation of waste-paper
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3629557/
https://www.ncbi.nlm.nih.gov/pubmed/23500568
http://dx.doi.org/10.1016/j.biortech.2013.01.084
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