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Scavenger Receptors Mediate the Role of SUMO and Ftz-f1 in Drosophila Steroidogenesis

SUMOylation participates in ecdysteroid biosynthesis at the onset of metamorphosis in Drosophila melanogaster. Silencing the Drosophila SUMO homologue smt3 in the prothoracic gland leads to reduced lipid content, low ecdysone titers, and a block in the larval–pupal transition. Here we show that the...

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Autores principales: Talamillo, Ana, Herboso, Leire, Pirone, Lucia, Pérez, Coralia, González, Monika, Sánchez, Jonatan, Mayor, Ugo, Lopitz-Otsoa, Fernando, Rodriguez, Manuel S., Sutherland, James D., Barrio, Rosa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3630131/
https://www.ncbi.nlm.nih.gov/pubmed/23637637
http://dx.doi.org/10.1371/journal.pgen.1003473
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author Talamillo, Ana
Herboso, Leire
Pirone, Lucia
Pérez, Coralia
González, Monika
Sánchez, Jonatan
Mayor, Ugo
Lopitz-Otsoa, Fernando
Rodriguez, Manuel S.
Sutherland, James D.
Barrio, Rosa
author_facet Talamillo, Ana
Herboso, Leire
Pirone, Lucia
Pérez, Coralia
González, Monika
Sánchez, Jonatan
Mayor, Ugo
Lopitz-Otsoa, Fernando
Rodriguez, Manuel S.
Sutherland, James D.
Barrio, Rosa
author_sort Talamillo, Ana
collection PubMed
description SUMOylation participates in ecdysteroid biosynthesis at the onset of metamorphosis in Drosophila melanogaster. Silencing the Drosophila SUMO homologue smt3 in the prothoracic gland leads to reduced lipid content, low ecdysone titers, and a block in the larval–pupal transition. Here we show that the SR-BI family of Scavenger Receptors mediates SUMO functions. Reduced levels of Snmp1 compromise lipid uptake in the prothoracic gland. In addition, overexpression of Snmp1 is able to recover lipid droplet levels in the smt3 knockdown prothoracic gland cells. Snmp1 expression depends on Ftz-f1 (an NR5A-type orphan nuclear receptor), the expression of which, in turn, depends on SUMO. Furthermore, we show by in vitro and in vivo experiments that Ftz-f1 is SUMOylated. RNAi–mediated knockdown of ftz-f1 phenocopies that of smt3 at the larval to pupal transition, thus Ftz-f1 is an interesting candidate to mediate some of the functions of SUMO at the onset of metamorphosis. Additionally, we demonstrate that the role of SUMOylation, Ftz-f1, and the Scavenger Receptors in lipid capture and mobilization is conserved in other steroidogenic tissues such as the follicle cells of the ovary. smt3 knockdown, as well as ftz-f1 or Scavenger knockdown, depleted the lipid content of the follicle cells, which could be rescued by Snmp1 overexpression. Therefore, our data provide new insights into the regulation of metamorphosis via lipid homeostasis, showing that Drosophila Smt3, Ftz-f1, and SR-BIs are part of a general mechanism for uptake of lipids such as cholesterol, required during development in steroidogenic tissues.
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spelling pubmed-36301312013-05-01 Scavenger Receptors Mediate the Role of SUMO and Ftz-f1 in Drosophila Steroidogenesis Talamillo, Ana Herboso, Leire Pirone, Lucia Pérez, Coralia González, Monika Sánchez, Jonatan Mayor, Ugo Lopitz-Otsoa, Fernando Rodriguez, Manuel S. Sutherland, James D. Barrio, Rosa PLoS Genet Research Article SUMOylation participates in ecdysteroid biosynthesis at the onset of metamorphosis in Drosophila melanogaster. Silencing the Drosophila SUMO homologue smt3 in the prothoracic gland leads to reduced lipid content, low ecdysone titers, and a block in the larval–pupal transition. Here we show that the SR-BI family of Scavenger Receptors mediates SUMO functions. Reduced levels of Snmp1 compromise lipid uptake in the prothoracic gland. In addition, overexpression of Snmp1 is able to recover lipid droplet levels in the smt3 knockdown prothoracic gland cells. Snmp1 expression depends on Ftz-f1 (an NR5A-type orphan nuclear receptor), the expression of which, in turn, depends on SUMO. Furthermore, we show by in vitro and in vivo experiments that Ftz-f1 is SUMOylated. RNAi–mediated knockdown of ftz-f1 phenocopies that of smt3 at the larval to pupal transition, thus Ftz-f1 is an interesting candidate to mediate some of the functions of SUMO at the onset of metamorphosis. Additionally, we demonstrate that the role of SUMOylation, Ftz-f1, and the Scavenger Receptors in lipid capture and mobilization is conserved in other steroidogenic tissues such as the follicle cells of the ovary. smt3 knockdown, as well as ftz-f1 or Scavenger knockdown, depleted the lipid content of the follicle cells, which could be rescued by Snmp1 overexpression. Therefore, our data provide new insights into the regulation of metamorphosis via lipid homeostasis, showing that Drosophila Smt3, Ftz-f1, and SR-BIs are part of a general mechanism for uptake of lipids such as cholesterol, required during development in steroidogenic tissues. Public Library of Science 2013-04-18 /pmc/articles/PMC3630131/ /pubmed/23637637 http://dx.doi.org/10.1371/journal.pgen.1003473 Text en © 2013 Talamillo et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Talamillo, Ana
Herboso, Leire
Pirone, Lucia
Pérez, Coralia
González, Monika
Sánchez, Jonatan
Mayor, Ugo
Lopitz-Otsoa, Fernando
Rodriguez, Manuel S.
Sutherland, James D.
Barrio, Rosa
Scavenger Receptors Mediate the Role of SUMO and Ftz-f1 in Drosophila Steroidogenesis
title Scavenger Receptors Mediate the Role of SUMO and Ftz-f1 in Drosophila Steroidogenesis
title_full Scavenger Receptors Mediate the Role of SUMO and Ftz-f1 in Drosophila Steroidogenesis
title_fullStr Scavenger Receptors Mediate the Role of SUMO and Ftz-f1 in Drosophila Steroidogenesis
title_full_unstemmed Scavenger Receptors Mediate the Role of SUMO and Ftz-f1 in Drosophila Steroidogenesis
title_short Scavenger Receptors Mediate the Role of SUMO and Ftz-f1 in Drosophila Steroidogenesis
title_sort scavenger receptors mediate the role of sumo and ftz-f1 in drosophila steroidogenesis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3630131/
https://www.ncbi.nlm.nih.gov/pubmed/23637637
http://dx.doi.org/10.1371/journal.pgen.1003473
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