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Regulation of Fibrochondrogenesis of Mesenchymal Stem Cells in an Integrated Microfluidic Platform Embedded with Biomimetic Nanofibrous Scaffolds

In native fibrocartilage, mechanotransduction allows the cells to perceive the physical microenvironment not only through topographical cues from the extracellular matrix, but also through mechanical cues, such as interstitial flow. To create a microenvironment that simultaneously integrates nanotop...

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Detalles Bibliográficos
Autores principales: Zhong, Weiliang, Zhang, Weiguo, Wang, Shouyu, Qin, Jianhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3630136/
https://www.ncbi.nlm.nih.gov/pubmed/23637803
http://dx.doi.org/10.1371/journal.pone.0061283
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author Zhong, Weiliang
Zhang, Weiguo
Wang, Shouyu
Qin, Jianhua
author_facet Zhong, Weiliang
Zhang, Weiguo
Wang, Shouyu
Qin, Jianhua
author_sort Zhong, Weiliang
collection PubMed
description In native fibrocartilage, mechanotransduction allows the cells to perceive the physical microenvironment not only through topographical cues from the extracellular matrix, but also through mechanical cues, such as interstitial flow. To create a microenvironment that simultaneously integrates nanotopography and flow stimulus, we developed a biomimetic microfluidic device embedded with aligned nanofibers to contain microchambers of different angles, which enabled the flow direction to form different angles with the fibers. Using this device, we investigated the effects of microfluidic and nanotopographical environment on the morphology and fibrochondrogenesis of mesenchymal stem cells (MSCs) and the involvement of RhoA/ROCK pathway and Yes-associated protein (YAP)/transcriptional co-activator with PDZ-binding motif (TAZ). The results showed that the flow direction perpendicular to aligned nanofibers was conducive to fibrochondrogenesis of MSCs. In addition, ROCK inhibitor and knockdown of YAP/TAZ disrupted fibrochondrogenic differentiation of MSCs. In conclusion, our data suggest the crucial role of mechanotransduction in regulating fibrochondrogenic differentiation of MSCs, which may be mediated by RhoA/ROCK pathway and YAP/TAZ.
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spelling pubmed-36301362013-05-01 Regulation of Fibrochondrogenesis of Mesenchymal Stem Cells in an Integrated Microfluidic Platform Embedded with Biomimetic Nanofibrous Scaffolds Zhong, Weiliang Zhang, Weiguo Wang, Shouyu Qin, Jianhua PLoS One Research Article In native fibrocartilage, mechanotransduction allows the cells to perceive the physical microenvironment not only through topographical cues from the extracellular matrix, but also through mechanical cues, such as interstitial flow. To create a microenvironment that simultaneously integrates nanotopography and flow stimulus, we developed a biomimetic microfluidic device embedded with aligned nanofibers to contain microchambers of different angles, which enabled the flow direction to form different angles with the fibers. Using this device, we investigated the effects of microfluidic and nanotopographical environment on the morphology and fibrochondrogenesis of mesenchymal stem cells (MSCs) and the involvement of RhoA/ROCK pathway and Yes-associated protein (YAP)/transcriptional co-activator with PDZ-binding motif (TAZ). The results showed that the flow direction perpendicular to aligned nanofibers was conducive to fibrochondrogenesis of MSCs. In addition, ROCK inhibitor and knockdown of YAP/TAZ disrupted fibrochondrogenic differentiation of MSCs. In conclusion, our data suggest the crucial role of mechanotransduction in regulating fibrochondrogenic differentiation of MSCs, which may be mediated by RhoA/ROCK pathway and YAP/TAZ. Public Library of Science 2013-04-18 /pmc/articles/PMC3630136/ /pubmed/23637803 http://dx.doi.org/10.1371/journal.pone.0061283 Text en © 2013 Zhong et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhong, Weiliang
Zhang, Weiguo
Wang, Shouyu
Qin, Jianhua
Regulation of Fibrochondrogenesis of Mesenchymal Stem Cells in an Integrated Microfluidic Platform Embedded with Biomimetic Nanofibrous Scaffolds
title Regulation of Fibrochondrogenesis of Mesenchymal Stem Cells in an Integrated Microfluidic Platform Embedded with Biomimetic Nanofibrous Scaffolds
title_full Regulation of Fibrochondrogenesis of Mesenchymal Stem Cells in an Integrated Microfluidic Platform Embedded with Biomimetic Nanofibrous Scaffolds
title_fullStr Regulation of Fibrochondrogenesis of Mesenchymal Stem Cells in an Integrated Microfluidic Platform Embedded with Biomimetic Nanofibrous Scaffolds
title_full_unstemmed Regulation of Fibrochondrogenesis of Mesenchymal Stem Cells in an Integrated Microfluidic Platform Embedded with Biomimetic Nanofibrous Scaffolds
title_short Regulation of Fibrochondrogenesis of Mesenchymal Stem Cells in an Integrated Microfluidic Platform Embedded with Biomimetic Nanofibrous Scaffolds
title_sort regulation of fibrochondrogenesis of mesenchymal stem cells in an integrated microfluidic platform embedded with biomimetic nanofibrous scaffolds
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3630136/
https://www.ncbi.nlm.nih.gov/pubmed/23637803
http://dx.doi.org/10.1371/journal.pone.0061283
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