Cargando…
Insulators Target Active Genes to Transcription Factories and Polycomb-Repressed Genes to Polycomb Bodies
Polycomb bodies are foci of Polycomb proteins in which different Polycomb target genes are thought to co-localize in the nucleus, looping out from their chromosomal context. We have shown previously that insulators, not Polycomb response elements (PREs), mediate associations among Polycomb Group (Pc...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3630138/ https://www.ncbi.nlm.nih.gov/pubmed/23637616 http://dx.doi.org/10.1371/journal.pgen.1003436 |
_version_ | 1782266663825047552 |
---|---|
author | Li, Hua-Bing Ohno, Katsuhito Gui, Hongxing Pirrotta, Vincenzo |
author_facet | Li, Hua-Bing Ohno, Katsuhito Gui, Hongxing Pirrotta, Vincenzo |
author_sort | Li, Hua-Bing |
collection | PubMed |
description | Polycomb bodies are foci of Polycomb proteins in which different Polycomb target genes are thought to co-localize in the nucleus, looping out from their chromosomal context. We have shown previously that insulators, not Polycomb response elements (PREs), mediate associations among Polycomb Group (PcG) targets to form Polycomb bodies. Here we use live imaging and 3C interactions to show that transgenes containing PREs and endogenous PcG-regulated genes are targeted by insulator proteins to different nuclear structures depending on their state of activity. When two genes are repressed, they co-localize in Polycomb bodies. When both are active, they are targeted to transcription factories in a fashion dependent on Trithorax and enhancer specificity as well as the insulator protein CTCF. In the absence of CTCF, assembly of Polycomb bodies is essentially reduced to those representing genomic clusters of Polycomb target genes. The critical role of Trithorax suggests that stable association with a specialized transcription factory underlies the cellular memory of the active state. |
format | Online Article Text |
id | pubmed-3630138 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36301382013-05-01 Insulators Target Active Genes to Transcription Factories and Polycomb-Repressed Genes to Polycomb Bodies Li, Hua-Bing Ohno, Katsuhito Gui, Hongxing Pirrotta, Vincenzo PLoS Genet Research Article Polycomb bodies are foci of Polycomb proteins in which different Polycomb target genes are thought to co-localize in the nucleus, looping out from their chromosomal context. We have shown previously that insulators, not Polycomb response elements (PREs), mediate associations among Polycomb Group (PcG) targets to form Polycomb bodies. Here we use live imaging and 3C interactions to show that transgenes containing PREs and endogenous PcG-regulated genes are targeted by insulator proteins to different nuclear structures depending on their state of activity. When two genes are repressed, they co-localize in Polycomb bodies. When both are active, they are targeted to transcription factories in a fashion dependent on Trithorax and enhancer specificity as well as the insulator protein CTCF. In the absence of CTCF, assembly of Polycomb bodies is essentially reduced to those representing genomic clusters of Polycomb target genes. The critical role of Trithorax suggests that stable association with a specialized transcription factory underlies the cellular memory of the active state. Public Library of Science 2013-04-18 /pmc/articles/PMC3630138/ /pubmed/23637616 http://dx.doi.org/10.1371/journal.pgen.1003436 Text en © 2013 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Li, Hua-Bing Ohno, Katsuhito Gui, Hongxing Pirrotta, Vincenzo Insulators Target Active Genes to Transcription Factories and Polycomb-Repressed Genes to Polycomb Bodies |
title | Insulators Target Active Genes to Transcription Factories and Polycomb-Repressed Genes to Polycomb Bodies |
title_full | Insulators Target Active Genes to Transcription Factories and Polycomb-Repressed Genes to Polycomb Bodies |
title_fullStr | Insulators Target Active Genes to Transcription Factories and Polycomb-Repressed Genes to Polycomb Bodies |
title_full_unstemmed | Insulators Target Active Genes to Transcription Factories and Polycomb-Repressed Genes to Polycomb Bodies |
title_short | Insulators Target Active Genes to Transcription Factories and Polycomb-Repressed Genes to Polycomb Bodies |
title_sort | insulators target active genes to transcription factories and polycomb-repressed genes to polycomb bodies |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3630138/ https://www.ncbi.nlm.nih.gov/pubmed/23637616 http://dx.doi.org/10.1371/journal.pgen.1003436 |
work_keys_str_mv | AT lihuabing insulatorstargetactivegenestotranscriptionfactoriesandpolycombrepressedgenestopolycombbodies AT ohnokatsuhito insulatorstargetactivegenestotranscriptionfactoriesandpolycombrepressedgenestopolycombbodies AT guihongxing insulatorstargetactivegenestotranscriptionfactoriesandpolycombrepressedgenestopolycombbodies AT pirrottavincenzo insulatorstargetactivegenestotranscriptionfactoriesandpolycombrepressedgenestopolycombbodies |