Cargando…
Variation of Expression Levels of Seven Housekeeping Genes at Different Life-History Stages in Porphyra yezoensis
In order to identify the optimal internal control for relative real-time PCR when studying target gene expression in the red alga Porphyra yezoensis, we quantified the expression of seven housekeeping genes (18S ribosomal RNA, 30S ribosomal protein S8, Polyubiquitin-2, Glyceraldehyde-3-phosphate deh...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3630157/ https://www.ncbi.nlm.nih.gov/pubmed/23637763 http://dx.doi.org/10.1371/journal.pone.0060740 |
_version_ | 1782266668408373248 |
---|---|
author | Wu, Xiaojie Huang, Aiyou Xu, Meiling Wang, Chao Jia, Zhaojun Wang, Guangce Niu, Jianfeng |
author_facet | Wu, Xiaojie Huang, Aiyou Xu, Meiling Wang, Chao Jia, Zhaojun Wang, Guangce Niu, Jianfeng |
author_sort | Wu, Xiaojie |
collection | PubMed |
description | In order to identify the optimal internal control for relative real-time PCR when studying target gene expression in the red alga Porphyra yezoensis, we quantified the expression of seven housekeeping genes (18S ribosomal RNA, 30S ribosomal protein S8, Polyubiquitin-2, Glyceraldehyde-3-phosphate dehydrogenase, Elongation factor 1-alpha, Beta-tubulin and Actin 3) at different life-history stages. Absolute quantification was done by normalization to total RNA quantity and by normalization to genomic DNA quantity. We used these two normalization approaches, comparing the differences of expression levels of all candidate housekeeping genes between any two generations and across three life-history stages (filamentous sporophytes, leafy gametophytes and conchospores). We found GAPDH had the best stability in all cases and we recommend that GAPDH be considered as a potential internal control for gene expression studies at different life-history stages in P. yezoensis. |
format | Online Article Text |
id | pubmed-3630157 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36301572013-05-01 Variation of Expression Levels of Seven Housekeeping Genes at Different Life-History Stages in Porphyra yezoensis Wu, Xiaojie Huang, Aiyou Xu, Meiling Wang, Chao Jia, Zhaojun Wang, Guangce Niu, Jianfeng PLoS One Research Article In order to identify the optimal internal control for relative real-time PCR when studying target gene expression in the red alga Porphyra yezoensis, we quantified the expression of seven housekeeping genes (18S ribosomal RNA, 30S ribosomal protein S8, Polyubiquitin-2, Glyceraldehyde-3-phosphate dehydrogenase, Elongation factor 1-alpha, Beta-tubulin and Actin 3) at different life-history stages. Absolute quantification was done by normalization to total RNA quantity and by normalization to genomic DNA quantity. We used these two normalization approaches, comparing the differences of expression levels of all candidate housekeeping genes between any two generations and across three life-history stages (filamentous sporophytes, leafy gametophytes and conchospores). We found GAPDH had the best stability in all cases and we recommend that GAPDH be considered as a potential internal control for gene expression studies at different life-history stages in P. yezoensis. Public Library of Science 2013-04-18 /pmc/articles/PMC3630157/ /pubmed/23637763 http://dx.doi.org/10.1371/journal.pone.0060740 Text en © 2013 Wu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wu, Xiaojie Huang, Aiyou Xu, Meiling Wang, Chao Jia, Zhaojun Wang, Guangce Niu, Jianfeng Variation of Expression Levels of Seven Housekeeping Genes at Different Life-History Stages in Porphyra yezoensis |
title | Variation of Expression Levels of Seven Housekeeping Genes at Different Life-History Stages in Porphyra yezoensis
|
title_full | Variation of Expression Levels of Seven Housekeeping Genes at Different Life-History Stages in Porphyra yezoensis
|
title_fullStr | Variation of Expression Levels of Seven Housekeeping Genes at Different Life-History Stages in Porphyra yezoensis
|
title_full_unstemmed | Variation of Expression Levels of Seven Housekeeping Genes at Different Life-History Stages in Porphyra yezoensis
|
title_short | Variation of Expression Levels of Seven Housekeeping Genes at Different Life-History Stages in Porphyra yezoensis
|
title_sort | variation of expression levels of seven housekeeping genes at different life-history stages in porphyra yezoensis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3630157/ https://www.ncbi.nlm.nih.gov/pubmed/23637763 http://dx.doi.org/10.1371/journal.pone.0060740 |
work_keys_str_mv | AT wuxiaojie variationofexpressionlevelsofsevenhousekeepinggenesatdifferentlifehistorystagesinporphyrayezoensis AT huangaiyou variationofexpressionlevelsofsevenhousekeepinggenesatdifferentlifehistorystagesinporphyrayezoensis AT xumeiling variationofexpressionlevelsofsevenhousekeepinggenesatdifferentlifehistorystagesinporphyrayezoensis AT wangchao variationofexpressionlevelsofsevenhousekeepinggenesatdifferentlifehistorystagesinporphyrayezoensis AT jiazhaojun variationofexpressionlevelsofsevenhousekeepinggenesatdifferentlifehistorystagesinporphyrayezoensis AT wangguangce variationofexpressionlevelsofsevenhousekeepinggenesatdifferentlifehistorystagesinporphyrayezoensis AT niujianfeng variationofexpressionlevelsofsevenhousekeepinggenesatdifferentlifehistorystagesinporphyrayezoensis |