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Cell-free microRNAs: potential biomarkers in need of standardized reporting

MicroRNAs are abundantly present and surprisingly stable in multiple biological fluids. These findings have been followed by numerous reverse transcription real-time quantitative PCR (RT-qPCR)-based reports revealing the clinical potential of using microRNA levels in body fluids as a biomarker of di...

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Detalles Bibliográficos
Autores principales: Kirschner, Michaela B., van Zandwijk, Nico, Reid, Glen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3630323/
https://www.ncbi.nlm.nih.gov/pubmed/23626598
http://dx.doi.org/10.3389/fgene.2013.00056
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author Kirschner, Michaela B.
van Zandwijk, Nico
Reid, Glen
author_facet Kirschner, Michaela B.
van Zandwijk, Nico
Reid, Glen
author_sort Kirschner, Michaela B.
collection PubMed
description MicroRNAs are abundantly present and surprisingly stable in multiple biological fluids. These findings have been followed by numerous reverse transcription real-time quantitative PCR (RT-qPCR)-based reports revealing the clinical potential of using microRNA levels in body fluids as a biomarker of disease. Despite a rapidly increasing body of literature, the field has failed to adopt a set of standardized criteria for reporting the methodology used in the quantification of cell-free microRNAs. Not only do many studies based on RT-qPCR fail to address the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) criteria but frequently there is also a distinct lack of detail in descriptions of sample source and RNA isolation. As a direct result, it is often impossible to compare the results of different studies, which in turn, hinders progress in the field. To address this point, we propose a simple set of criteria to be used in conjunction with MIQE to reveal the true potential of cell-free microRNAs as biomarkers.
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spelling pubmed-36303232013-04-26 Cell-free microRNAs: potential biomarkers in need of standardized reporting Kirschner, Michaela B. van Zandwijk, Nico Reid, Glen Front Genet Genetics MicroRNAs are abundantly present and surprisingly stable in multiple biological fluids. These findings have been followed by numerous reverse transcription real-time quantitative PCR (RT-qPCR)-based reports revealing the clinical potential of using microRNA levels in body fluids as a biomarker of disease. Despite a rapidly increasing body of literature, the field has failed to adopt a set of standardized criteria for reporting the methodology used in the quantification of cell-free microRNAs. Not only do many studies based on RT-qPCR fail to address the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) criteria but frequently there is also a distinct lack of detail in descriptions of sample source and RNA isolation. As a direct result, it is often impossible to compare the results of different studies, which in turn, hinders progress in the field. To address this point, we propose a simple set of criteria to be used in conjunction with MIQE to reveal the true potential of cell-free microRNAs as biomarkers. Frontiers Media S.A. 2013-04-19 /pmc/articles/PMC3630323/ /pubmed/23626598 http://dx.doi.org/10.3389/fgene.2013.00056 Text en Copyright © Kirschner, van Zandwijk and Reid. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.
spellingShingle Genetics
Kirschner, Michaela B.
van Zandwijk, Nico
Reid, Glen
Cell-free microRNAs: potential biomarkers in need of standardized reporting
title Cell-free microRNAs: potential biomarkers in need of standardized reporting
title_full Cell-free microRNAs: potential biomarkers in need of standardized reporting
title_fullStr Cell-free microRNAs: potential biomarkers in need of standardized reporting
title_full_unstemmed Cell-free microRNAs: potential biomarkers in need of standardized reporting
title_short Cell-free microRNAs: potential biomarkers in need of standardized reporting
title_sort cell-free micrornas: potential biomarkers in need of standardized reporting
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3630323/
https://www.ncbi.nlm.nih.gov/pubmed/23626598
http://dx.doi.org/10.3389/fgene.2013.00056
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