Dynamin-SNARE interactions control trans-SNARE formation in intracellular membrane fusion

The fundamental processes of membrane fission and fusion determine size and copy numbers of intracellular organelles. While SNARE proteins and tethering complexes mediate intracellular membrane fusion, fission requires the presence of dynamin or dynamin-related proteins. Here we study these reaction...

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Autores principales: Alpadi, Kannan, Kulkarni, Aditya, Namjoshi, Sarita, Srinivasan, Sankaranarayanan, Sippel, Katherine H., Ayscough, Kathryn, Zieger, Martin, Schmidt, Andrea, Mayer, Andreas, Evangelista, Michael, Quiocho, Florante A., Peters, Christopher
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3630463/
https://www.ncbi.nlm.nih.gov/pubmed/23591871
http://dx.doi.org/10.1038/ncomms2724
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author Alpadi, Kannan
Kulkarni, Aditya
Namjoshi, Sarita
Srinivasan, Sankaranarayanan
Sippel, Katherine H.
Ayscough, Kathryn
Zieger, Martin
Schmidt, Andrea
Mayer, Andreas
Evangelista, Michael
Quiocho, Florante A.
Peters, Christopher
author_facet Alpadi, Kannan
Kulkarni, Aditya
Namjoshi, Sarita
Srinivasan, Sankaranarayanan
Sippel, Katherine H.
Ayscough, Kathryn
Zieger, Martin
Schmidt, Andrea
Mayer, Andreas
Evangelista, Michael
Quiocho, Florante A.
Peters, Christopher
author_sort Alpadi, Kannan
collection PubMed
description The fundamental processes of membrane fission and fusion determine size and copy numbers of intracellular organelles. While SNARE proteins and tethering complexes mediate intracellular membrane fusion, fission requires the presence of dynamin or dynamin-related proteins. Here we study these reactions in native yeast vacuoles and find that the yeast dynamin homolog Vps1 is not only an essential part of the fission machinery, but also controls membrane fusion by generating an active Q(a) SNARE- tethering complex pool, which is essential for trans-SNARE formation. Our findings provide new insight into the role of dynamins in membrane fusion by directly acting on SNARE proteins.
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spelling pubmed-36304632013-10-16 Dynamin-SNARE interactions control trans-SNARE formation in intracellular membrane fusion Alpadi, Kannan Kulkarni, Aditya Namjoshi, Sarita Srinivasan, Sankaranarayanan Sippel, Katherine H. Ayscough, Kathryn Zieger, Martin Schmidt, Andrea Mayer, Andreas Evangelista, Michael Quiocho, Florante A. Peters, Christopher Nat Commun Article The fundamental processes of membrane fission and fusion determine size and copy numbers of intracellular organelles. While SNARE proteins and tethering complexes mediate intracellular membrane fusion, fission requires the presence of dynamin or dynamin-related proteins. Here we study these reactions in native yeast vacuoles and find that the yeast dynamin homolog Vps1 is not only an essential part of the fission machinery, but also controls membrane fusion by generating an active Q(a) SNARE- tethering complex pool, which is essential for trans-SNARE formation. Our findings provide new insight into the role of dynamins in membrane fusion by directly acting on SNARE proteins. 2013 /pmc/articles/PMC3630463/ /pubmed/23591871 http://dx.doi.org/10.1038/ncomms2724 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Alpadi, Kannan
Kulkarni, Aditya
Namjoshi, Sarita
Srinivasan, Sankaranarayanan
Sippel, Katherine H.
Ayscough, Kathryn
Zieger, Martin
Schmidt, Andrea
Mayer, Andreas
Evangelista, Michael
Quiocho, Florante A.
Peters, Christopher
Dynamin-SNARE interactions control trans-SNARE formation in intracellular membrane fusion
title Dynamin-SNARE interactions control trans-SNARE formation in intracellular membrane fusion
title_full Dynamin-SNARE interactions control trans-SNARE formation in intracellular membrane fusion
title_fullStr Dynamin-SNARE interactions control trans-SNARE formation in intracellular membrane fusion
title_full_unstemmed Dynamin-SNARE interactions control trans-SNARE formation in intracellular membrane fusion
title_short Dynamin-SNARE interactions control trans-SNARE formation in intracellular membrane fusion
title_sort dynamin-snare interactions control trans-snare formation in intracellular membrane fusion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3630463/
https://www.ncbi.nlm.nih.gov/pubmed/23591871
http://dx.doi.org/10.1038/ncomms2724
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