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The MASTER (methylation-assisted tailorable ends rational) ligation method for seamless DNA assembly
Techniques for assembly of designed DNA sequences are important for synthetic biology. So far, a few methods have been developed towards high-throughput seamless DNA assembly in vitro, including both the homologous sequences-based system and the type IIS-mediated system. Here, we describe a novel me...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3632120/ https://www.ncbi.nlm.nih.gov/pubmed/23444142 http://dx.doi.org/10.1093/nar/gkt122 |