Degradation of initiator tRNA(Met) by Xrn1/2 via its accumulation in the nucleus of heat-treated HeLa cells

Stress response mechanisms that modulate the dynamics of tRNA degradation and accumulation from the cytoplasm to the nucleus have been studied in yeast, the rat hepatoma and human cells. In the current study, we investigated tRNA degradation and accumulation in HeLa cells under various forms of stre...

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Autores principales: Watanabe, Kazunori, Miyagawa, Ryu, Tomikawa, Chie, Mizuno, Rie, Takahashi, Akihisa, Hori, Hiroyuki, Ijiri, Kenichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3632136/
https://www.ncbi.nlm.nih.gov/pubmed/23471000
http://dx.doi.org/10.1093/nar/gkt153
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author Watanabe, Kazunori
Miyagawa, Ryu
Tomikawa, Chie
Mizuno, Rie
Takahashi, Akihisa
Hori, Hiroyuki
Ijiri, Kenichi
author_facet Watanabe, Kazunori
Miyagawa, Ryu
Tomikawa, Chie
Mizuno, Rie
Takahashi, Akihisa
Hori, Hiroyuki
Ijiri, Kenichi
author_sort Watanabe, Kazunori
collection PubMed
description Stress response mechanisms that modulate the dynamics of tRNA degradation and accumulation from the cytoplasm to the nucleus have been studied in yeast, the rat hepatoma and human cells. In the current study, we investigated tRNA degradation and accumulation in HeLa cells under various forms of stress. We found that initiator tRNA(Met) (tRNA(iMet)) was specifically degraded under heat stress. Two exonucleases, Xrn1 and Xrn2, are involved in the degradation of tRNA(iMet) in the cytoplasm and the nucleus, respectively. In addition to degradation, we observed accumulation of tRNA(iMet) in the nucleus. We also found that the mammalian target of rapamycin (mTOR), which regulates tRNA trafficking in yeast, is partially phosphorylated at Ser2448 in the presence of rapamycin and/or during heat stress. Our results suggest phosphorylation of mTOR may correlate with accumulation of tRNA(iMet) in heat-treated HeLa cells.
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spelling pubmed-36321362013-04-22 Degradation of initiator tRNA(Met) by Xrn1/2 via its accumulation in the nucleus of heat-treated HeLa cells Watanabe, Kazunori Miyagawa, Ryu Tomikawa, Chie Mizuno, Rie Takahashi, Akihisa Hori, Hiroyuki Ijiri, Kenichi Nucleic Acids Res RNA Stress response mechanisms that modulate the dynamics of tRNA degradation and accumulation from the cytoplasm to the nucleus have been studied in yeast, the rat hepatoma and human cells. In the current study, we investigated tRNA degradation and accumulation in HeLa cells under various forms of stress. We found that initiator tRNA(Met) (tRNA(iMet)) was specifically degraded under heat stress. Two exonucleases, Xrn1 and Xrn2, are involved in the degradation of tRNA(iMet) in the cytoplasm and the nucleus, respectively. In addition to degradation, we observed accumulation of tRNA(iMet) in the nucleus. We also found that the mammalian target of rapamycin (mTOR), which regulates tRNA trafficking in yeast, is partially phosphorylated at Ser2448 in the presence of rapamycin and/or during heat stress. Our results suggest phosphorylation of mTOR may correlate with accumulation of tRNA(iMet) in heat-treated HeLa cells. Oxford University Press 2013-04 2013-03-06 /pmc/articles/PMC3632136/ /pubmed/23471000 http://dx.doi.org/10.1093/nar/gkt153 Text en © The Author(s) 2013. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle RNA
Watanabe, Kazunori
Miyagawa, Ryu
Tomikawa, Chie
Mizuno, Rie
Takahashi, Akihisa
Hori, Hiroyuki
Ijiri, Kenichi
Degradation of initiator tRNA(Met) by Xrn1/2 via its accumulation in the nucleus of heat-treated HeLa cells
title Degradation of initiator tRNA(Met) by Xrn1/2 via its accumulation in the nucleus of heat-treated HeLa cells
title_full Degradation of initiator tRNA(Met) by Xrn1/2 via its accumulation in the nucleus of heat-treated HeLa cells
title_fullStr Degradation of initiator tRNA(Met) by Xrn1/2 via its accumulation in the nucleus of heat-treated HeLa cells
title_full_unstemmed Degradation of initiator tRNA(Met) by Xrn1/2 via its accumulation in the nucleus of heat-treated HeLa cells
title_short Degradation of initiator tRNA(Met) by Xrn1/2 via its accumulation in the nucleus of heat-treated HeLa cells
title_sort degradation of initiator trna(met) by xrn1/2 via its accumulation in the nucleus of heat-treated hela cells
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3632136/
https://www.ncbi.nlm.nih.gov/pubmed/23471000
http://dx.doi.org/10.1093/nar/gkt153
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