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Diaphragm Atrophy and Contractile Dysfunction in a Murine Model of Pulmonary Hypertension

Pulmonary hypertension (PH) causes loss of body weight and inspiratory (diaphragm) muscle dysfunction. A model of PH induced by drug (monocrotaline, MCT) has been extensively used in mice to examine the etiology of PH. However, it is unclear if PH induced by MCT in mice reproduces the loss of body w...

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Autores principales: Ahn, Bumsoo, Empinado, Hyacinth M., Al-Rajhi, Monsour, Judge, Andrew R., Ferreira, Leonardo F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3632558/
https://www.ncbi.nlm.nih.gov/pubmed/23614054
http://dx.doi.org/10.1371/journal.pone.0062702
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author Ahn, Bumsoo
Empinado, Hyacinth M.
Al-Rajhi, Monsour
Judge, Andrew R.
Ferreira, Leonardo F.
author_facet Ahn, Bumsoo
Empinado, Hyacinth M.
Al-Rajhi, Monsour
Judge, Andrew R.
Ferreira, Leonardo F.
author_sort Ahn, Bumsoo
collection PubMed
description Pulmonary hypertension (PH) causes loss of body weight and inspiratory (diaphragm) muscle dysfunction. A model of PH induced by drug (monocrotaline, MCT) has been extensively used in mice to examine the etiology of PH. However, it is unclear if PH induced by MCT in mice reproduces the loss of body weight and diaphragm muscle dysfunction seen in patients. This is a pre-requisite for widespread use of mice to examine mechanisms of cachexia and diaphragm abnormalities in PH. Thus, we measured body and soleus muscle weight, food intake, and diaphragm contractile properties in mice after 6–8 weeks of saline (control) or MCT (600 mg/kg) injections. Body weight progressively decreased in PH mice, while food intake was similar in both groups. PH decreased (P<0.05) diaphragm maximal isometric specific force, maximal shortening velocity, and peak power. Protein carbonyls in whole-diaphragm lysates and the abundance of select myofibrillar proteins were unchanged by PH. Our findings show diaphragm isometric and isotonic contractile abnormalities in a murine model of PH induced by MCT. Overall, the murine model of PH elicited by MCT mimics loss of body weight and diaphragm muscle weakness reported in PH patients.
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spelling pubmed-36325582013-04-23 Diaphragm Atrophy and Contractile Dysfunction in a Murine Model of Pulmonary Hypertension Ahn, Bumsoo Empinado, Hyacinth M. Al-Rajhi, Monsour Judge, Andrew R. Ferreira, Leonardo F. PLoS One Research Article Pulmonary hypertension (PH) causes loss of body weight and inspiratory (diaphragm) muscle dysfunction. A model of PH induced by drug (monocrotaline, MCT) has been extensively used in mice to examine the etiology of PH. However, it is unclear if PH induced by MCT in mice reproduces the loss of body weight and diaphragm muscle dysfunction seen in patients. This is a pre-requisite for widespread use of mice to examine mechanisms of cachexia and diaphragm abnormalities in PH. Thus, we measured body and soleus muscle weight, food intake, and diaphragm contractile properties in mice after 6–8 weeks of saline (control) or MCT (600 mg/kg) injections. Body weight progressively decreased in PH mice, while food intake was similar in both groups. PH decreased (P<0.05) diaphragm maximal isometric specific force, maximal shortening velocity, and peak power. Protein carbonyls in whole-diaphragm lysates and the abundance of select myofibrillar proteins were unchanged by PH. Our findings show diaphragm isometric and isotonic contractile abnormalities in a murine model of PH induced by MCT. Overall, the murine model of PH elicited by MCT mimics loss of body weight and diaphragm muscle weakness reported in PH patients. Public Library of Science 2013-04-22 /pmc/articles/PMC3632558/ /pubmed/23614054 http://dx.doi.org/10.1371/journal.pone.0062702 Text en © 2013 Ahn et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ahn, Bumsoo
Empinado, Hyacinth M.
Al-Rajhi, Monsour
Judge, Andrew R.
Ferreira, Leonardo F.
Diaphragm Atrophy and Contractile Dysfunction in a Murine Model of Pulmonary Hypertension
title Diaphragm Atrophy and Contractile Dysfunction in a Murine Model of Pulmonary Hypertension
title_full Diaphragm Atrophy and Contractile Dysfunction in a Murine Model of Pulmonary Hypertension
title_fullStr Diaphragm Atrophy and Contractile Dysfunction in a Murine Model of Pulmonary Hypertension
title_full_unstemmed Diaphragm Atrophy and Contractile Dysfunction in a Murine Model of Pulmonary Hypertension
title_short Diaphragm Atrophy and Contractile Dysfunction in a Murine Model of Pulmonary Hypertension
title_sort diaphragm atrophy and contractile dysfunction in a murine model of pulmonary hypertension
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3632558/
https://www.ncbi.nlm.nih.gov/pubmed/23614054
http://dx.doi.org/10.1371/journal.pone.0062702
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