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Stac3 Is a Novel Regulator of Skeletal Muscle Development in Mice
The goal of this study was to identify novel factors that mediate skeletal muscle development or function. We began the study by searching the gene expression databases for genes that have no known functions but are preferentially expressed in skeletal muscle. This search led to the identification o...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3633831/ https://www.ncbi.nlm.nih.gov/pubmed/23626854 http://dx.doi.org/10.1371/journal.pone.0062760 |
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author | Reinholt, Brad M. Ge, Xiaomei Cong, Xiaofei Gerrard, David E. Jiang, Honglin |
author_facet | Reinholt, Brad M. Ge, Xiaomei Cong, Xiaofei Gerrard, David E. Jiang, Honglin |
author_sort | Reinholt, Brad M. |
collection | PubMed |
description | The goal of this study was to identify novel factors that mediate skeletal muscle development or function. We began the study by searching the gene expression databases for genes that have no known functions but are preferentially expressed in skeletal muscle. This search led to the identification of the Src homology three (SH3) domain and cysteine rich (C1) domain 3 (Stac3) gene. We experimentally confirmed that Stac3 mRNA was predominantly expressed in skeletal muscle. We determined if Stac3 plays a role in skeletal muscle development or function by generating Stac3 knockout mice. All Stac3 homozygous mutant mice were found dead at birth, were never seen move, and had a curved body and dropping forelimbs. These mice had marked abnormalities in skeletal muscles throughout the body, including central location of myonuclei, decreased number but increased cross-sectional area of myofibers, decreased number and size of myofibrils, disarrayed myofibrils, and streaming Z-lines. These phenotypes demonstrate that the Stac3 gene plays a critical role in skeletal muscle development and function in mice. |
format | Online Article Text |
id | pubmed-3633831 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36338312013-04-26 Stac3 Is a Novel Regulator of Skeletal Muscle Development in Mice Reinholt, Brad M. Ge, Xiaomei Cong, Xiaofei Gerrard, David E. Jiang, Honglin PLoS One Research Article The goal of this study was to identify novel factors that mediate skeletal muscle development or function. We began the study by searching the gene expression databases for genes that have no known functions but are preferentially expressed in skeletal muscle. This search led to the identification of the Src homology three (SH3) domain and cysteine rich (C1) domain 3 (Stac3) gene. We experimentally confirmed that Stac3 mRNA was predominantly expressed in skeletal muscle. We determined if Stac3 plays a role in skeletal muscle development or function by generating Stac3 knockout mice. All Stac3 homozygous mutant mice were found dead at birth, were never seen move, and had a curved body and dropping forelimbs. These mice had marked abnormalities in skeletal muscles throughout the body, including central location of myonuclei, decreased number but increased cross-sectional area of myofibers, decreased number and size of myofibrils, disarrayed myofibrils, and streaming Z-lines. These phenotypes demonstrate that the Stac3 gene plays a critical role in skeletal muscle development and function in mice. Public Library of Science 2013-04-23 /pmc/articles/PMC3633831/ /pubmed/23626854 http://dx.doi.org/10.1371/journal.pone.0062760 Text en © 2013 Reinholt et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Reinholt, Brad M. Ge, Xiaomei Cong, Xiaofei Gerrard, David E. Jiang, Honglin Stac3 Is a Novel Regulator of Skeletal Muscle Development in Mice |
title | Stac3 Is a Novel Regulator of Skeletal Muscle Development in Mice |
title_full | Stac3 Is a Novel Regulator of Skeletal Muscle Development in Mice |
title_fullStr | Stac3 Is a Novel Regulator of Skeletal Muscle Development in Mice |
title_full_unstemmed | Stac3 Is a Novel Regulator of Skeletal Muscle Development in Mice |
title_short | Stac3 Is a Novel Regulator of Skeletal Muscle Development in Mice |
title_sort | stac3 is a novel regulator of skeletal muscle development in mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3633831/ https://www.ncbi.nlm.nih.gov/pubmed/23626854 http://dx.doi.org/10.1371/journal.pone.0062760 |
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